Literature summary for 3.1.1.41 extracted from

Takimoto, A.; Takakura, T.; Tani, H.; Yagi, S.; Mitsushima, K.
Batch production of deacetyl 7-aminocephalosporanic acid by immobilized cephalosporin-C deacetylase (2004), Appl. Microbiol. Biotechnol., 65, 263-267.

Search for more literature for 3.1.1.41

Application

Application	Comment	Organism
synthesis	production of deacetyl 7-aminocephalosporanic acid as precursor for the synthesis of cefcapene pivoxil hydrochloride (Flomox)	Bacillus subtilis

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli JM109	Bacillus subtilis

General Stability

General Stability	Organism
immobilized enzyme is very stable and exhibited only a slight decrease in activity upon repeated use	Bacillus subtilis

Inhibitors

Inhibitors	Comment	Organism	Structure
additional information	no inhibition by reaction products	Bacillus subtilis

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
cephalosporin C + H2O	Bacillus subtilis	-	deacetylcephalosporin C + acetate	-	?
cephalosporin C + H2O	Bacillus subtilis SHS0133	-	deacetylcephalosporin C + acetate	-	?

Organism

Organism	UniProt	Comment	Textmining
Bacillus subtilis	-	recombinant enzyme from Escherichia coli immobilized on an anion exchange resin	-
Bacillus subtilis SHS0133	-	recombinant enzyme from Escherichia coli immobilized on an anion exchange resin	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant protein purified to 90%	Bacillus subtilis

Storage Stability

Storage Stability	Organism
5°C, pH 8.0, immobilized enzyme, 2 years, 4% loss of activity	Bacillus subtilis

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
7-aminocephalosporanic acid + H2O	-	Bacillus subtilis	deacetyl 7-aminocephalosporanic acid + acetate	reaction product is not less than 97.5% pure, only a negligible amount of deacetyl 7-aminocephalospornolactone detected	?
7-aminocephalosporanic acid + H2O	-	Bacillus subtilis SHS0133	deacetyl 7-aminocephalosporanic acid + acetate	reaction product is not less than 97.5% pure, only a negligible amount of deacetyl 7-aminocephalospornolactone detected	?
cephalosporin C + H2O	-	Bacillus subtilis	deacetylcephalosporin C + acetate	-	?
cephalosporin C + H2O	-	Bacillus subtilis SHS0133	deacetylcephalosporin C + acetate	-	?

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
50	-	immobilized and free enzyme fully stable	Bacillus subtilis

pH Stability

pH Stability	pH Stability Maximum	Comment	Organism
4	11	immobilized enzyme stable for at least 60 min at 20°C	Bacillus subtilis
10	-	free enzyme shows highest activity after incubation at pH10 for 60 min at 20°C, about 15% of activity remaining after incubation at pH4	Bacillus subtilis

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Release 2023.1 (January 2023)