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Literature summary for 3.1.1.41 extracted from

  • Takimoto, A.; Mitsushima, K.; Yagi, S.; Sonoyama, T.
    Purification, characterization and partial amino acid sequences of a novel cephalosporin-C deacetylase from Bacillus subtilis (1994), J. Ferment. Bioeng., 77, 17-22.
No PubMed abstract available

Inhibitors

Inhibitors Comment Organism Structure
acetate competitive; weak Bacillus subtilis
Cu2+
-
Bacillus subtilis
deacetyl-7-aminocephalosporanic acid weak noncompetitive Bacillus subtilis
DFP
-
Bacillus subtilis
additional information the weak product inhibitors do not prevent the completion of the deacetylation Bacillus subtilis
N-tosyl-L-Lys chloromethyl ketone
-
Bacillus subtilis
Zn2+
-
Bacillus subtilis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1
-
p-nitrophenyl acetate
-
Bacillus subtilis
6.5
-
7-aminocephalosporanic acid strain ATCC6633 Bacillus subtilis
7.9
-
7-aminocephalosporanic acid strain SHS 0133, FERM BP-2755 Bacillus subtilis
11.1
-
cephalotin
-
Bacillus subtilis
11.5
-
cephaloglycin
-
Bacillus subtilis
13.9
-
cephapirin
-
Bacillus subtilis
24
-
cephalosporin C
-
Bacillus subtilis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
35000
-
8 * 35000, strain SHS 0133, SDS-PAGE Bacillus subtilis
150000
-
strain ATCC6633 Bacillus subtilis
280000
-
strain SHS 0133, gel filtration Bacillus subtilis

Organism

Organism UniProt Comment Textmining
Bacillus subtilis
-
-
-
Bacillus subtilis
-
strain ATCC6633
-
Bacillus subtilis ATCC 6633
-
strain ATCC6633
-
Bacillus subtilis SHS 0133
-
-
-

Purification (Commentary)

Purification (Comment) Organism
strain SHS 0133, FERM BP-2755 Bacillus subtilis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
187.4
-
hydrolysis of p-nitrophenyl acetate Bacillus subtilis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
7-aminocephalosporanic acid + H2O
-
Bacillus subtilis acetate + deacetyl-7-aminocephalosporanic acid
-
?
7-aminocephalosporanic acid + H2O
-
Bacillus subtilis ATCC 6633 acetate + deacetyl-7-aminocephalosporanic acid
-
?
7-aminocephalosporanic acid + H2O
-
Bacillus subtilis SHS 0133 acetate + deacetyl-7-aminocephalosporanic acid
-
?
cephaloglycin + H2O
-
Bacillus subtilis acetate + (6R,7R)-7-{[(2R)-2-amino-2-phenylacetyl]amino}-3-(hydroxymethyl)-8-oxo-5-thia-1-azabicyclo[4.2.0]oct-2-ene-2-carboxylic acid
-
?
cephaloglycin + H2O
-
Bacillus subtilis ATCC 6633 acetate + (6R,7R)-7-{[(2R)-2-amino-2-phenylacetyl]amino}-3-(hydroxymethyl)-8-oxo-5-thia-1-azabicyclo[4.2.0]oct-2-ene-2-carboxylic acid
-
?
cephaloglycin + H2O
-
Bacillus subtilis SHS 0133 acetate + (6R,7R)-7-{[(2R)-2-amino-2-phenylacetyl]amino}-3-(hydroxymethyl)-8-oxo-5-thia-1-azabicyclo[4.2.0]oct-2-ene-2-carboxylic acid
-
?
cephalosporin C + H2O
-
Bacillus subtilis deacetylcephalosporin C + acetate
-
?
cephalothin + H2O
-
Bacillus subtilis acetate + deacetylcephalothin
-
?
cephalothin + H2O
-
Bacillus subtilis ATCC 6633 acetate + deacetylcephalothin
-
?
cephalothin + H2O
-
Bacillus subtilis SHS 0133 acetate + deacetylcephalothin
-
?
cephapirin + H2O
-
Bacillus subtilis acetate + deacetylcephapirin
-
?
cephapirin + H2O
-
Bacillus subtilis ATCC 6633 acetate + deacetylcephapirin
-
?
cephapirin + H2O
-
Bacillus subtilis SHS 0133 acetate + deacetylcephapirin
-
?
p-nitrophenyl acetate + H2O
-
Bacillus subtilis 4-nitrophenol + acetate
-
?
p-nitrophenyl acetate + H2O
-
Bacillus subtilis ATCC 6633 4-nitrophenol + acetate
-
?
p-nitrophenyl acetate + H2O
-
Bacillus subtilis SHS 0133 4-nitrophenol + acetate
-
?

Subunits

Subunits Comment Organism
octamer 8 * 35000, strain SHS 0133, SDS-PAGE Bacillus subtilis
tetramer strain ATCC6633 Bacillus subtilis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55
-
strain SHS 0133, FERM BP-2755 Bacillus subtilis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30
-
pH 7.0, stable for at least 1 month Bacillus subtilis
60
-
pH 7.0, 30 min, stable up to Bacillus subtilis
65
-
pH 7.0, 30 min, 70% loss of activity Bacillus subtilis
70
-
pH 7.0, 30 min, complete loss of activity Bacillus subtilis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
55
-
cephalothin
-
Bacillus subtilis
80
-
cephapirin
-
Bacillus subtilis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
strain ATCC6633 Bacillus subtilis
8 8.5
-
Bacillus subtilis

pH Stability

pH Stability pH Stability Maximum Comment Organism
7 10 37°C, 30 min, stable Bacillus subtilis