Inhibitors | Comment | Organism | Structure |
---|---|---|---|
acetate | competitive; weak | Bacillus subtilis | |
Cu2+ | - |
Bacillus subtilis | |
deacetyl-7-aminocephalosporanic acid | weak noncompetitive | Bacillus subtilis | |
DFP | - |
Bacillus subtilis | |
additional information | the weak product inhibitors do not prevent the completion of the deacetylation | Bacillus subtilis | |
N-tosyl-L-Lys chloromethyl ketone | - |
Bacillus subtilis | |
Zn2+ | - |
Bacillus subtilis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1 | - |
p-nitrophenyl acetate | - |
Bacillus subtilis | |
6.5 | - |
7-aminocephalosporanic acid | strain ATCC6633 | Bacillus subtilis | |
7.9 | - |
7-aminocephalosporanic acid | strain SHS 0133, FERM BP-2755 | Bacillus subtilis | |
11.1 | - |
cephalotin | - |
Bacillus subtilis | |
11.5 | - |
cephaloglycin | - |
Bacillus subtilis | |
13.9 | - |
cephapirin | - |
Bacillus subtilis | |
24 | - |
cephalosporin C | - |
Bacillus subtilis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
35000 | - |
8 * 35000, strain SHS 0133, SDS-PAGE | Bacillus subtilis |
150000 | - |
strain ATCC6633 | Bacillus subtilis |
280000 | - |
strain SHS 0133, gel filtration | Bacillus subtilis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | - |
- |
- |
Bacillus subtilis | - |
strain ATCC6633 | - |
Bacillus subtilis ATCC 6633 | - |
strain ATCC6633 | - |
Bacillus subtilis SHS 0133 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
strain SHS 0133, FERM BP-2755 | Bacillus subtilis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
187.4 | - |
hydrolysis of p-nitrophenyl acetate | Bacillus subtilis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
7-aminocephalosporanic acid + H2O | - |
Bacillus subtilis | acetate + deacetyl-7-aminocephalosporanic acid | - |
? | |
7-aminocephalosporanic acid + H2O | - |
Bacillus subtilis ATCC 6633 | acetate + deacetyl-7-aminocephalosporanic acid | - |
? | |
7-aminocephalosporanic acid + H2O | - |
Bacillus subtilis SHS 0133 | acetate + deacetyl-7-aminocephalosporanic acid | - |
? | |
cephaloglycin + H2O | - |
Bacillus subtilis | acetate + (6R,7R)-7-{[(2R)-2-amino-2-phenylacetyl]amino}-3-(hydroxymethyl)-8-oxo-5-thia-1-azabicyclo[4.2.0]oct-2-ene-2-carboxylic acid | - |
? | |
cephaloglycin + H2O | - |
Bacillus subtilis ATCC 6633 | acetate + (6R,7R)-7-{[(2R)-2-amino-2-phenylacetyl]amino}-3-(hydroxymethyl)-8-oxo-5-thia-1-azabicyclo[4.2.0]oct-2-ene-2-carboxylic acid | - |
? | |
cephaloglycin + H2O | - |
Bacillus subtilis SHS 0133 | acetate + (6R,7R)-7-{[(2R)-2-amino-2-phenylacetyl]amino}-3-(hydroxymethyl)-8-oxo-5-thia-1-azabicyclo[4.2.0]oct-2-ene-2-carboxylic acid | - |
? | |
cephalosporin C + H2O | - |
Bacillus subtilis | deacetylcephalosporin C + acetate | - |
? | |
cephalothin + H2O | - |
Bacillus subtilis | acetate + deacetylcephalothin | - |
? | |
cephalothin + H2O | - |
Bacillus subtilis ATCC 6633 | acetate + deacetylcephalothin | - |
? | |
cephalothin + H2O | - |
Bacillus subtilis SHS 0133 | acetate + deacetylcephalothin | - |
? | |
cephapirin + H2O | - |
Bacillus subtilis | acetate + deacetylcephapirin | - |
? | |
cephapirin + H2O | - |
Bacillus subtilis ATCC 6633 | acetate + deacetylcephapirin | - |
? | |
cephapirin + H2O | - |
Bacillus subtilis SHS 0133 | acetate + deacetylcephapirin | - |
? | |
p-nitrophenyl acetate + H2O | - |
Bacillus subtilis | 4-nitrophenol + acetate | - |
? | |
p-nitrophenyl acetate + H2O | - |
Bacillus subtilis ATCC 6633 | 4-nitrophenol + acetate | - |
? | |
p-nitrophenyl acetate + H2O | - |
Bacillus subtilis SHS 0133 | 4-nitrophenol + acetate | - |
? |
Subunits | Comment | Organism |
---|---|---|
octamer | 8 * 35000, strain SHS 0133, SDS-PAGE | Bacillus subtilis |
tetramer | strain ATCC6633 | Bacillus subtilis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
strain SHS 0133, FERM BP-2755 | Bacillus subtilis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
pH 7.0, stable for at least 1 month | Bacillus subtilis |
60 | - |
pH 7.0, 30 min, stable up to | Bacillus subtilis |
65 | - |
pH 7.0, 30 min, 70% loss of activity | Bacillus subtilis |
70 | - |
pH 7.0, 30 min, complete loss of activity | Bacillus subtilis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
55 | - |
cephalothin | - |
Bacillus subtilis | |
80 | - |
cephapirin | - |
Bacillus subtilis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
strain ATCC6633 | Bacillus subtilis |
8 | 8.5 | - |
Bacillus subtilis |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
7 | 10 | 37°C, 30 min, stable | Bacillus subtilis |