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Literature summary for 3.1.1.4 extracted from

  • Bonfim, V.L.; Ponce-Soto, L.A.; Martins de Souza, D.; Souza, G.H.; Baldasso, P.A.; Eberlin, M.N.; Marangoni, S.
    Structural and functional characterization of myotoxin, Cr-IV 1, a phospholipase A2 D49 from the venom of the snake Calloselasma rhodostoma (2008), Biologicals, 36, 168-176.
    View publication on PubMed

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
Calloselasma rhodostoma
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
13871
-
x * 13871, mass spectrometry, x * 14000, SDS-PAGE Calloselasma rhodostoma
14000
-
x * 13871, mass spectrometry, x * 14000, SDS-PAGE Calloselasma rhodostoma

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Calloselasma rhodostoma Cr-IV 1 shows a rapid cytolytic effect on mouse skeletal muscle myoblasts and myotubes in culture. Cr-IV 1 induces myonecrosis and edema upon intramuscular and intravenous injections, respectively, in mice ?
-
?

Organism

Organism UniProt Comment Textmining
Calloselasma rhodostoma
-
-
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme from venom by gel filtration and C18 reverse phase HPLC Calloselasma rhodostoma

Source Tissue

Source Tissue Comment Organism Textmining
venom
-
Calloselasma rhodostoma
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitro-3-(octanoyloxy)benzoic acid + H2O a synthetic chromogenic substrate Calloselasma rhodostoma ?
-
?
additional information Cr-IV 1 shows a rapid cytolytic effect on mouse skeletal muscle myoblasts and myotubes in culture. Cr-IV 1 induces myonecrosis and edema upon intramuscular and intravenous injections, respectively, in mice Calloselasma rhodostoma ?
-
?

Subunits

Subunits Comment Organism
? x * 13871, mass spectrometry, x * 14000, SDS-PAGE Calloselasma rhodostoma
More determination of the amino acid composition, overview Calloselasma rhodostoma

Synonyms

Synonyms Comment Organism
Cr-IV 1
-
Calloselasma rhodostoma
More Cr-IV 1 as a member of the D49 PLA2 family Calloselasma rhodostoma
Myotoxin
-
Calloselasma rhodostoma
phospholipase A2 D49
-
Calloselasma rhodostoma
PLA2
-
Calloselasma rhodostoma

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Calloselasma rhodostoma

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
assay at Calloselasma rhodostoma

pI Value

Organism Comment pI Value Maximum pI Value
Calloselasma rhodostoma sequence calculation
-
8