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Literature summary for 3.1.1.4 extracted from

  • Bonfim, V.L.; Ponce-Soto, L.A.; Novello, J.C.; Marangoni, S.
    Cytotoxic action in myoblasts and myotubes (C2C12) and enzymatic characterization of a new phospholipase A2 isoform (Bj-V) from Bothrops jararacussu venom (2006), Protein Pept. Lett., 13, 707-713.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
Cdcas F3 a crotapotin Bothrops jararacussu
Cdcas F4 a crotapotin Bothrops jararacussu
Cdcoll F3 a crotapotin Bothrops jararacussu
Cdcoll F4 a crotapotin Bothrops jararacussu
Cdt F7 a crotapotin Bothrops jararacussu
Cu2+ at 10 mM, diminishes activation in presence of 1 mM Ca2+, slight activation in absence of Ca2+ Bothrops jararacussu
Mg2+ at 10 mM, diminishes activation in presence of 1 mM Ca2+, poor activation in absence of Ca2+ Bothrops jararacussu
Zn2+ at 10 mM, diminishes activation in presence of 1 mM Ca2+, no activation in absence of Ca2+ Bothrops jararacussu

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
Bothrops jararacussu
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ required at 10 mM for full activity Bothrops jararacussu
Cu2+ at 10 mM, diminishes activation in presence of 1 mM Ca2+, slight activation in absence of Ca2+ Bothrops jararacussu
Mg2+ at 10 mM, diminishes activation in presence of 1 mM Ca2+, poor activation in absence of Ca2+ Bothrops jararacussu
Mn2+ activates at 10 mM in presence of 1 mM Ca2+ Bothrops jararacussu

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Bothrops jararacussu the enzyme is a component of the venom and causes cytotoxic lesions in murine skeletal muscle C2C12 myoblasts and myotubes, overview ?
-
?

Organism

Organism UniProt Comment Textmining
Bothrops jararacussu
-
isozyme Bj-V
-

Source Tissue

Source Tissue Comment Organism Textmining
venom
-
Bothrops jararacussu
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the enzyme is a component of the venom and causes cytotoxic lesions in murine skeletal muscle C2C12 myoblasts and myotubes, overview Bothrops jararacussu ?
-
?

Subunits

Subunits Comment Organism
dimer 2 * 14039.5, isozyme Bj-V, mass spectrometry and native PAGE Bothrops jararacussu

Synonyms

Synonyms Comment Organism
Bj-V
-
Bothrops jararacussu
PLA2
-
Bothrops jararacussu

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
35 40
-
Bothrops jararacussu

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.4
-
-
Bothrops jararacussu