Literature summary for 3.1.1.4 extracted from

Murakami, M.T.; Kuch, U.; Mebs, D.; Arni, R.K.
Crystallization and preliminary X-ray diffraction analysis of a novel Arg49 phospholipase A2 homologue from Zhaoermia mangshanensis venom (2007), Acta Crystallogr. Sect. F, 63, 605-607.

Crystallization (Commentary)

Crystallization (Comment)	Organism
purified enzyme, hanging-drop vapour-diffusion method, 0.001 ml of protein solution containing 30 mg/ml protein in 20 mM HEPES buffer, pH 7.5, is mixed with an equal volume of reservoir solution containing 0.2 M ammonium sulfate, 0.1 M sodium cacodylate trihydrate, pH 6.5, and 30% w/v polyethylene glycol 8000, room temperature, 2 months, X-ray diffraction structure determination and analysis at 2.05 A resolution	Protobothrops mangshanensis

Crystallization (Comment)

Organism

purified enzyme, hanging-drop vapour-diffusion method, 0.001 ml of protein solution containing 30 mg/ml protein in 20 mM HEPES buffer, pH 7.5, is mixed with an equal volume of reservoir solution containing 0.2 M ammonium sulfate, 0.1 M sodium cacodylate trihydrate, pH 6.5, and 30% w/v polyethylene glycol 8000, room temperature, 2 months, X-ray diffraction structure determination and analysis at 2.05 A resolution

Protobothrops mangshanensis

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
13972	-	2 * 13972	Protobothrops mangshanensis

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
additional information	Protobothrops mangshanensis	zhaoermiatoxin induces oedema and strong myonecrosis without detectable PLA2 catalytic activity, overview	?	-	?

Organism

Organism	UniProt	Comment	Textmining
Protobothrops mangshanensis	P84776	formerly Trimeresurus mangshanensis or Ermia mangshanensis	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
venom	-	Protobothrops mangshanensis	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
additional information	zhaoermiatoxin induces oedema and strong myonecrosis without detectable PLA2 catalytic activity, overview	Protobothrops mangshanensis	?	-	?

Subunits

Subunits	Comment	Organism
dimer	2 * 13972	Protobothrops mangshanensis

Synonyms

Synonyms	Comment	Organism
Arg49 phospholipase A2 homologue	-	Protobothrops mangshanensis
More	Arg49 phospholipase A2 homologue is a member of the PLA2-homologue family that possesses an arginine residue at position 49, probably arising from a secondary K49R substitution	Protobothrops mangshanensis