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Literature summary for 3.1.1.4 extracted from
- Enzymatic characterization of the major phospholipase A2 component of sea anemone (Aiptasia pallida) nematocyst venom (2000), Toxicon, 38, 931-943.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| Lipid | enzyme is surface activated by aggregated lipids such as mixed micelles of detergent and phospholipid | Exaiptasia diaphana |  |
| Triton X-100 | activates at 0.166 mM and 0.333 mM | Exaiptasia diaphana | |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ba2+ | inactivates | Exaiptasia diaphana | |
| Zn2+ | inhibits in the presence of Ca2+, inactivates in the absence of Ca2+ | Exaiptasia diaphana | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.65 | - |
L-alpha-dipalmitoyl phosphatidylcholine | pH 7.4, 40°C | Exaiptasia diaphana | |
| 1.5 | - |
Ca2+ | pH 7.4, 40°C | Exaiptasia diaphana | |
| 3.6 | - |
Mg2+ | pH 7.4, 40°C | Exaiptasia diaphana | |
| 12 | - |
Mn2+ | pH 7.4, 40°C | Exaiptasia diaphana | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | requirement | Exaiptasia diaphana | |
| Mg2+ | activates | Exaiptasia diaphana | |
| Mn2+ | activates | Exaiptasia diaphana | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Exaiptasia diaphana | - |
sea anemone | - |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D,L-alpha-dipalmitoyl phosphatidylcholine + H2O | - |
Exaiptasia diaphana | ? | - |
? | |
| L-alpha-dipalmitoyl phosphatidylcholine + H2O | - |
Exaiptasia diaphana | ? | - |
? | |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
unstable above | Exaiptasia diaphana |
| 56 | - |
50% loss of activity after 25 min | Exaiptasia diaphana |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.7 | - |
narrow pH optimum | Exaiptasia diaphana |
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Release 2023.1 (January 2023)
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