Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.1.1.35 extracted from

  • Giedraityte, G.; Kalediene, L.
    3,4-Dihydrocoumarin hydrolase from thermophilic Geobacillus sp. strain (2012), Rom. Biotechnol. Lett., 17, 7104-7113.
No PubMed abstract available
Search for more literature for 3.1.1.35

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.66
-
 gentisic acid gamma-lactone pH 7.0, 60°C Geobacillus sp.
0.81
-
 2-coumaranone pH 7.0, 60°C Geobacillus sp.
0.996
-
 3,4-dihydrocoumarin pH 7.0, 60°C Geobacillus sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
30000
-
 2 * 30000, SDS-PAGE Geobacillus sp.
70000
-
 gel filtration Geobacillus sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3,4-dihydrocoumarin + H2O Geobacillus sp. the enzyme is specific for 3,4-dihydrocoumarin melilotate
-
 ?
3,4-dihydrocoumarin + H2O Geobacillus sp. G27 the enzyme is specific for 3,4-dihydrocoumarin melilotate
-
 ?

Organism

Organism UniProt Comment Textmining
Geobacillus sp.
-
 isolated as a 1-naphthol assimilating microorganism
-
Geobacillus sp. G27
-
 isolated as a 1-naphthol assimilating microorganism
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme from strain G27 186fold to homogeneity by ammonium sulfate fractionation, anion exchange chromatography, and gel filtration Geobacillus sp.

Source Tissue

Source Tissue Comment Organism Textmining
cell culture growth culture medium containing peptone and with the addition of sodium succinate, benzoic acid or 1-naphthol as the supplementary sole carbon source for induction of lactone-hydrolyzing enzyme activity Geobacillus sp.
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
15.8
-
 purified enzyme, pH 7.0, 60°C Geobacillus sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-coumaranone low activity Geobacillus sp. ?
-
 ?
2-coumaranone low activity Geobacillus sp. G27 ?
-
 ?
3,4-dihydrocoumarin + H2O the enzyme is specific for 3,4-dihydrocoumarin Geobacillus sp. melilotate
-
 ?
3,4-dihydrocoumarin + H2O the enzyme is specific for 3,4-dihydrocoumarin Geobacillus sp. G27 melilotate
-
 ?
gentisic acid gamma-lactone low activity Geobacillus sp. ?
-
 ?
gentisic acid gamma-lactone low activity Geobacillus sp. G27 ?
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 30000, SDS-PAGE Geobacillus sp.

Synonyms

Synonyms Comment Organism
3,4-dihydrocoumarin hydrolase
-
 Geobacillus sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
 Geobacillus sp.

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 80 activity range, high activity at 50-65°C, profile overview Geobacillus sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
 purified native enzyme, fully stable for 20 min, half-life is 55 min, inactivation after 75 min Geobacillus sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
 Geobacillus sp.

pH Range

pH Minimum pH Maximum Comment Organism
5.5 9 activity range, high activity at pH 6.0-8.0, profile overview Geobacillus sp.

Expression

Organism Comment Expression
Geobacillus sp. the enzyme is induced by growth on 1-naphthol, although not by growth on D-glucose, benzoic acid, or succinate up