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Literature summary for 3.1.1.35 extracted from

  • Kataoka, M.; Honda, K.; Shimizu, S.
    A novel dihydrocoumarin hydrolase from Acinetobacter calcoaceticus: application to the production of useful compounds (2002), J. Mol. Catal. B, 19-20, 231-235.
No PubMed abstract available
Search for more literature for 3.1.1.35

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Acinetobacter calcoaceticus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.88
-
 methyl cetraxate hydrochloride
-
 Acinetobacter calcoaceticus
25.9
-
 DL-beta-acetylthioisobutyrate methyl ester
-
 Acinetobacter calcoaceticus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
dihydrocoumarin + H2O Acinetobacter calcoaceticus
-
 3-(2-hydroxyphenyl)propionic acid trivial name melilotate ?
dihydrocoumarin + H2O Acinetobacter calcoaceticus F46
-
 3-(2-hydroxyphenyl)propionic acid trivial name melilotate ?

Organism

Organism UniProt Comment Textmining
Acinetobacter calcoaceticus
-
-
-
Acinetobacter calcoaceticus F46
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Acinetobacter calcoaceticus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-chloro-5,5-dimethyl-1,3-cyclohexanedimedon + Br- + peracetic acid
-
 Acinetobacter calcoaceticus 2-chloro-2-bromo-5,5-dimethyl-1,3-cyclohexanedimedon + acetic acid + H2O
-
 ?
2-chloro-5,5-dimethyl-1,3-cyclohexanedimedon + Br- + peracetic acid
-
 Acinetobacter calcoaceticus F46 2-chloro-2-bromo-5,5-dimethyl-1,3-cyclohexanedimedon + acetic acid + H2O
-
 ?
3,4-dihydrocoumarin + H2O
-
 Acinetobacter calcoaceticus 3-(2-hydroxyphenyl)propionic acid
-
 ?
3,4-dihydrocoumarin + H2O
-
 Acinetobacter calcoaceticus F46 3-(2-hydroxyphenyl)propionic acid
-
 ?
dihydrocoumarin + H2O
-
 Acinetobacter calcoaceticus 3-(2-hydroxyphenyl)propionic acid trivial name melilotate ?
dihydrocoumarin + H2O
-
 Acinetobacter calcoaceticus F46 3-(2-hydroxyphenyl)propionic acid trivial name melilotate ?
DL-beta-acetylthioisobutyrate methyl ester + H2O stereospecific hydrolysis Acinetobacter calcoaceticus D-beta-acetylthioisobutyrate + methanol 99% enatiomeric excess ?
DL-beta-acetylthioisobutyrate methyl ester + H2O stereospecific hydrolysis Acinetobacter calcoaceticus F46 D-beta-acetylthioisobutyrate + methanol 99% enatiomeric excess ?
methyl cetraxate hydrochloride + H2O
-
 Acinetobacter calcoaceticus cetraxate hydrochloride + methanol
-
 ?
methyl cetraxate hydrochloride + H2O
-
 Acinetobacter calcoaceticus F46 cetraxate hydrochloride + methanol
-
 ?