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Literature summary for 3.1.1.35 extracted from
- 3,4-Dihydrocoumarin hydrolase with haloperoxidase activity from Acinetobacter calcoaceticus F46 (2000), Eur. J. Biochem., 267, 3-10.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| diisopropyl fluorophosphate | 1 mM, complete inhibition | Acinetobacter calcoaceticus |  |
| HgCl2 | 1 mM, complete inhibition | Acinetobacter calcoaceticus | |
| phenylmethanesulfonyl fluoride | 1 mM, 54% inhibition | Acinetobacter calcoaceticus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.81 | - |
3,4-dihydroxycoumarin | 30°C, pH 7.0 | Acinetobacter calcoaceticus | |
| 25.9 | - |
DL-beta-acetylthioisobutyrate methyl ester | 30°C, pH 7.0 | Acinetobacter calcoaceticus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 30000 | - |
2 * 30000, SDS-PAGE | Acinetobacter calcoaceticus |
| 56000 | - |
gel filtration | Acinetobacter calcoaceticus |
| 75000 | - |
gel filtration | Acinetobacter calcoaceticus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| dihydrocoumarin + H2O | Acinetobacter calcoaceticus | - |
3-(2-hydroxyphenyl)propionic acid | trivial name melilotate | ? | |
| dihydrocoumarin + H2O | Acinetobacter calcoaceticus F46 | - |
3-(2-hydroxyphenyl)propionic acid | trivial name melilotate | ? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Acinetobacter calcoaceticus | - |
- |
- |
| Acinetobacter calcoaceticus F46 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| ammonium sulfate, DEAE-Sephacel, Phenyl-Superose, Superdex 200 | Acinetobacter calcoaceticus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 199 | - |
bromination of 2-chloro-5,5-dimethyl-1,3-cyclohexanedimedon | Acinetobacter calcoaceticus |
| 1500 | - |
3,4-dihydrocoumarin hydrolysis | Acinetobacter calcoaceticus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-chloro-5,5-dimethyl-1,3-cyclohexanedimedon + Br- + acetic acid + H2O2 | - |
Acinetobacter calcoaceticus | 2-chloro-2-bromo-5,5-dimethyl-1,3-cyclohexanedimedon + acetic acid + H2O | - |
? | |
| 2-chloro-5,5-dimethyl-1,3-cyclohexanedimedon + Br- + acetic acid + H2O2 | - |
Acinetobacter calcoaceticus F46 | 2-chloro-2-bromo-5,5-dimethyl-1,3-cyclohexanedimedon + acetic acid + H2O | - |
? | |
| 3,4-dihydrocoumarin + H2O | - |
Acinetobacter calcoaceticus | 3-(2-hydroxyphenyl)propionic acid | - |
? | |
| 3,4-dihydrocoumarin + H2O | - |
Acinetobacter calcoaceticus F46 | 3-(2-hydroxyphenyl)propionic acid | - |
? | |
| dihydrocoumarin + H2O | - |
Acinetobacter calcoaceticus | 3-(2-hydroxyphenyl)propionic acid | trivial name melilotate | ? | |
| dihydrocoumarin + H2O | - |
Acinetobacter calcoaceticus F46 | 3-(2-hydroxyphenyl)propionic acid | trivial name melilotate | ? | |
| DL-beta-acetylthioisobutyrate methyl ester + H2O | stereospecific hydrolysis, 3.8% of activity with 3,4-dihydrocoumarin | Acinetobacter calcoaceticus | D-beta-acetylthioisobutyrate + methanol | 99% enatiomeric excess | ? | |
| DL-beta-acetylthioisobutyrate methyl ester + H2O | stereospecific hydrolysis, 3.8% of activity with 3,4-dihydrocoumarin | Acinetobacter calcoaceticus F46 | D-beta-acetylthioisobutyrate + methanol | 99% enatiomeric excess | ? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 30000, SDS-PAGE | Acinetobacter calcoaceticus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
- |
Acinetobacter calcoaceticus |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | 60 | approx. 90% of maximal activity at 20°C, approx. 40% of maximal activity at 60°C | Acinetobacter calcoaceticus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
- |
80 | no loss of activity after 30 min below 75°C, 31% loss of activity after 30 min at 80°C | Acinetobacter calcoaceticus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 8 | - |
Acinetobacter calcoaceticus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 10.5 | approx. 50% of maximal activity at pH 5.0, approx. 40% of maximal activity at pH 10.0 | Acinetobacter calcoaceticus |
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