Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.1.1.34 extracted from

  • Reimund, M.; Kovrov, O.; Olivecrona, G.; Lookene, A.
    Lipoprotein lipase activity and interactions studied in human plasma by isothermal titration calorimetry (2017), J. Lipid Res., 58, 279-288 .
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
apoC-II activation of LPL Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
angiopoietin-like protein 3 ANGPTL3 causes reduction of the activity of LPL via a two-step mechanism Homo sapiens
angiopoietin-like protein 4 ANGPTL4 causes reduction of the activity of LPL via a two-step mechanism. The N-terminal coiled-coil domain of human ANGPTL4, residues 26-184, is expressed in Escherichia coli and purified Homo sapiens
apoC-III inhibits the enzyme's lipolysis activity Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information initial rates and kinetics for complete hydrolysis of plasma lipids in postheparin plasma Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
Homo sapiens 5737
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
triacylglycerol + H2O Homo sapiens lipoproteins with high triglyceride diacylglycerol + a carboxylate
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens P06858
-
-

Source Tissue

Source Tissue Comment Organism Textmining
adipocyte
-
Homo sapiens
-
blood plasma
-
Homo sapiens
-
endothelium
-
Homo sapiens
-
additional information enzyme LPL is produced and secreted from parenchymal cells like adipocytes and myocytes for transport to the luminal side of the endothelium via interaction with the glycosylphosphatidylinositol-anchored high density lipoprotein binding protein 1 (GPIHBP1) Homo sapiens
-
myocyte
-
Homo sapiens
-
parenchyma
-
Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
triacylglycerol + H2O lipoproteins with high triglyceride Homo sapiens diacylglycerol + a carboxylate
-
?
triacylglycerol + H2O the lipase substrate used is plasma or VLDL with or without added ligands, enzyme source is postheparin plasma, lipoproteins with high triglyceride Homo sapiens diacylglycerol + a carboxylate
-
?

Synonyms

Synonyms Comment Organism
LPL
-
Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
assay at Homo sapiens

General Information

General Information Comment Organism
additional information enzyme ligand interaction analysis by isothermal titration calorimetry (ITC) using human plasma as substrate. ITC can be used for quantitative measurements of LPL activity and interactions under in vivo-like conditions, for comparisons of the properties of plasma samples from patients and control subjects as substrates for LPL, as well as for testing of drug candidates, method evaluation, overview Homo sapiens
physiological function the hydrolytic breakdown of plasma triglycerides by LPL at the capillary endothelium is a crucial event that contributes to control of the levels of triglycerides in plasma. LPL hydrolyzes triglycerides in plasma lipoproteins through a complex regulation mechanism Homo sapiens