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Literature summary for 3.1.1.34 extracted from

  • Basu, D.; Manjur, J.; Jin, W.
    Determination of lipoprotein lipase activity using a novel fluorescent lipase assay (2011), J. Lipid Res., 52, 826-832.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.1.34

Activating Compound

Activating Compound Comment Organism Structure
apoC-II apoC-II activates the enzyme 3.5fold in a saturable fashion. Heat-inactivated rat serum is often used as a source of apoC-II for activation of lipoprotein lipase Mus musculus
apoC-II apoC-II activates the enzyme 3.5fold in a saturable fashion. Heat-inactivated rat serum is often used as a source of apoC-II for activation of lipoprotein lipase Bos taurus
heparin addition of heparin increases enzyme activity minimally by about 6% Mus musculus
heparin addition of heparin increases enzyme activity minimally by about 6% Bos taurus
NaCl addition of NaCl increases the reaction rate with EnzChek lipase substrate dramatically with the highest rate, 46% higher than that without salt, occurring at 0.15 M Mus musculus
NaCl addition of NaCl increases the reaction rate with EnzChek lipase substrate dramatically with the highest rate, 46% higher than that without salt, occurring at 0.15 M Bos taurus

Inhibitors

Inhibitors Comment Organism Structure
angiopoietin-like protein-4
-
 Bos taurus
angiopoietin-like protein-4
-
 Mus musculus
heat-inactivated rat serum heat-inactivated rat serum added from 0-10%, decreases the enzyme activity by 12%. HIS also contains lipoprotein lipase-inhibitory factors such as angiopoietin-like protein-3, angiopoietin-like protein-4, apoC-I, and apoC-III Bos taurus
heat-inactivated rat serum heat-inactivated rat serum added from 0-10%, decreases the enzyme activity by 12%. HIS also contains lipoprotein lipase-inhibitory factors such as angiopoietin-like protein-3, angiopoietin-like protein-4, apoC-I, and apoC-III Mus musculus
NaCl 1 M NaCl inhibits the reaction with triolein by 80%, but there is no inhibition of lipoprotein lipase activity by NaCl if apoC-II is not used in the assay Bos taurus
NaCl 1 M NaCl inhibits the reaction with triolein by 80%, but there is no inhibition of lipoprotein lipase activity by NaCl if apoC-II is not used in the assay Mus musculus
tetrahydrolipstatin active-site inhibitor Bos taurus
tetrahydrolipstatin active-site inhibitor Mus musculus

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ addition of calcium increases enzyme activity minimally by about 6% Mus musculus
Ca2+ addition of calcium increases enzyme activity minimally by about 6% Bos taurus

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-
Mus musculus
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
blood plasma
-
 Mus musculus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1,2-O-dilauryl-DL-glycero-3-glutaric acid-(6-methylresorufin ester) + H2O
-
 Mus musculus ?
-
 ?
1,2-O-dilauryl-DL-glycero-3-glutaric acid-(6-methylresorufin ester) + H2O
-
 Bos taurus ?
-
 ?
EnzChek lipase substrate + H2O a commercially available BODIPY, Dabcyl-labeled triglyceride analog substrate, C58H85BF2N6O6 Mus musculus ?
-
 ?
EnzChek lipase substrate + H2O a commercially available BODIPY, Dabcyl-labeled triglyceride analog substrate, C58H85BF2N6O6 Bos taurus ?
-
 ?
triolein + H2O
-
 Mus musculus oleate + diolein
-
 ?
triolein + H2O
-
 Bos taurus oleate + diolein
-
 ?

Synonyms

Synonyms Comment Organism
LPL
-
 Mus musculus
LPL
-
 Bos taurus