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Literature summary for 3.1.1.3 extracted from

  • David, L.; Guo, X.J.; Villard, C.; Moulin, A.; Puigserver, A.
    Purification and molecular cloning of porcine intestinal glycerol-ester hydrolase--evidence for its identity with carboxylesterase (1998), Eur. J. Biochem., 257, 142-148.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
additional information active towards triacylglycerols containing short fatty acids from C2 to C6 Sus scrofa

Cloned(Commentary)

Cloned (Comment) Organism
-
Sus scrofa

Crystallization (Commentary)

Crystallization (Comment) Organism
-
Sus scrofa

Inhibitors

Inhibitors Comment Organism Structure
diisopropyl fluorophosphate
-
Sus scrofa
phenylmethylsulfonyl fluoride
-
Sus scrofa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
59917
-
x * 59917, calculation from nucleotide sequence Sus scrofa
60000
-
4 * 60000, SDS-PAGE Sus scrofa
240000
-
gel filtration Sus scrofa

Organism

Organism UniProt Comment Textmining
Sus scrofa
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
side-chain modification contains three glucosamine residues and three mannose residues per subunit Sus scrofa

Purification (Commentary)

Purification (Comment) Organism
-
Sus scrofa

Source Tissue

Source Tissue Comment Organism Textmining
intestinal mucosa
-
Sus scrofa
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
290
-
-
Sus scrofa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
methylbutyrate + H2O
-
Sus scrofa methanol + butyric acid
-
?
monoacetylglycerol + H2O
-
Sus scrofa acetic acid + glycerol
-
?
additional information
-
Sus scrofa ?
-
?
p-nitrophenyl acetate + H2O
-
Sus scrofa p-nitrophenol + acetic acid
-
?
triacetylglycerol + H2O
-
Sus scrofa acetic acid + ?
-
?
tributyrin + H2O
-
Sus scrofa butyric acid + ?
-
?
vitamin A acetate + H2O
-
Sus scrofa ?
-
?

Subunits

Subunits Comment Organism
? x * 59917, calculation from nucleotide sequence Sus scrofa
tetramer 4 * 60000, SDS-PAGE Sus scrofa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
hydrolysis of tributyrin Sus scrofa