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Literature summary for 3.1.1.3 extracted from

  • Che Omar, I.; Hayashi, M.; Nagai, S.
    Purification and some properties of a thermostable lipase from Humicola lanuginosa No. 3 (1987), Agric. Biol. Chem., 51, 37-45.
No PubMed abstract available
Search for more literature for 3.1.1.3

Inhibitors

Inhibitors Comment Organism Structure
Co2+
-
 Thermomyces lanuginosus
Cu2+
-
 Thermomyces lanuginosus
EDTA 1 mM, 11% inhibition Thermomyces lanuginosus
Hg2+
-
 Thermomyces lanuginosus
Mg2+ 1 mM, 17% inhibition Thermomyces lanuginosus
Ni2+
-
 Thermomyces lanuginosus
Sn2+
-
 Thermomyces lanuginosus
Zn2+ 1 mM, 17% inhibition Thermomyces lanuginosus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information Km-value: 14.2 mg/ml for trilaurin Thermomyces lanuginosus

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ enhances activity Thermomyces lanuginosus
K+ enhances activity Thermomyces lanuginosus
Li+ enhances activity Thermomyces lanuginosus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
39000
-
 gel filtration Thermomyces lanuginosus

Organism

Organism UniProt Comment Textmining
Thermomyces lanuginosus
-
 No. 3
-
Thermomyces lanuginosus No. 3
-
 No. 3
-

Purification (Commentary)

Purification (Comment) Organism
-
 Thermomyces lanuginosus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Thermomyces lanuginosus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1,2-dioleoylglycerol + H2O
-
 Thermomyces lanuginosus oleic acid + ?
-
 ?
1,2-dioleoylglycerol + H2O
-
 Thermomyces lanuginosus No. 3 oleic acid + ?
-
 ?
methyl linoleate + H2O
-
 Thermomyces lanuginosus methanol + linolic acid
-
 ?
methyl linolenate + H2O
-
 Thermomyces lanuginosus methanol + linoleic acid
-
 ?
methyl oleate + H2O
-
 Thermomyces lanuginosus methanol + oleic acid
-
 ?
methyl oleate + H2O
-
 Thermomyces lanuginosus No. 3 methanol + oleic acid
-
 ?
additional information 1,3-positional specific lipase Thermomyces lanuginosus ?
-
 ?
additional information 1,3-positional specific lipase Thermomyces lanuginosus No. 3 ?
-
 ?
polyethylene sorbitan monooleate + H2O i.e. Tween 80 Thermomyces lanuginosus ?
-
 ?
Span 85 + H2O
-
 Thermomyces lanuginosus ?
-
 ?
trilaurin + H2O
-
 Thermomyces lanuginosus lauric acid + ?
-
 ?
trilaurin + H2O
-
 Thermomyces lanuginosus No. 3 lauric acid + ?
-
 ?
trioleoylglycerol + H2O
-
 Thermomyces lanuginosus oleic acid + ?
-
 ?
trioleoylglycerol + H2O
-
 Thermomyces lanuginosus No. 3 oleic acid + ?
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 39000, SDS-PAGE Thermomyces lanuginosus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
-
 Thermomyces lanuginosus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
45
-
 24 h, stable Thermomyces lanuginosus
60
-
 20 h, stable Thermomyces lanuginosus
65
-
 60 min, stable Thermomyces lanuginosus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
 Thermomyces lanuginosus