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Literature summary for 3.1.1.3 extracted from
- Enzymatic esterification of eugenol and benzoic acid by a novel chitosan-chitin nanowhiskers supported Rhizomucor miehei lipase Process optimization and kinetic assessments (2018), Enzyme Microb. Technol., 108, 42-52 .
Application
| Application | Comment | Organism |
|---|---|---|
| synthesis | synthesis of eugenyl benzoate by esterification of eugenol and benzoic acid catalyzed by the chitosan-chitin nanowhiskers supported lipase. Under optimum conditions, a maximum conversion yield of 66% at 50°C in 5 h using 3 mg/ml of lipase, and a substrate molar ratio (eugenol: benzoic acid) of 3:1 is obtained. The lipase is reusable up to 8 esterification cycles and shows higher thermal stability than free enzyme | Rhizomucor miehei |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| eugenol | substrate inhibition | Rhizomucor miehei |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 34.04 | - |
benzoic acid | chitosan-chitin nanowhiskers supported lipase, pH not specified in the publication, 50°C | Rhizomucor miehei | |
| 138.28 | - |
eugenol | chitosan-chitin nanowhiskers supported lipase, pH not specified in the publication, 50°C | Rhizomucor miehei | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rhizomucor miehei | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| commercial preparation | - |
Rhizomucor miehei | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| eugenol + benzoic acid | - |
Rhizomucor miehei | eugenyl benzoate | - |
? | |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.67 | - |
eugenol | chitosan-chitin nanowhiskers supported lipase, pH not specified in the publication, 50°C | Rhizomucor miehei | |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 438.6 | - |
eugenol | chitosan-chitin nanowhiskers supported lipase, pH not specified in the publication, 50°C | Rhizomucor miehei | |
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