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Literature summary for 3.1.1.3 extracted from

  • Tong, X.; Busk, P.K.; Lange, L.
    Characterization of a new sn-1,3-regioselective triacylglycerol lipase from Malbranchea cinnamomea (2016), Biotechnol. Appl. Biochem., 63, 471-478 .
    View publication on PubMed
Search for more literature for 3.1.1.3

Cloned(Commentary)

Cloned (Comment) Organism
expression in Pichia pastoris Malbranchea cinnamomea

Inhibitors

Inhibitors Comment Organism Structure
additional information not inhibitory: Na+, K+, Ca2+, Mg2+, Cu2+, Co2+, Fe2+ at 1 mM Malbranchea cinnamomea
Zn2+ 1 mM, 40% residual activity Malbranchea cinnamomea

Organic Solvent Stability

Organic Solvent Comment Organism
Methanol not inhibitory at 10% v/v. At 20% v/v, not inhibitory up to 30°C and 57% residual activity at 40°C Malbranchea cinnamomea

Organism

Organism UniProt Comment Textmining
Malbranchea cinnamomea A0A0F7KL65
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl butanoate + H2O 20% of the activity with -nitrophenyl myristate Malbranchea cinnamomea 4-nitrophenol + butanoate
-
 ?
4-nitrophenyl decanoate + H2O 81% of the activity with -nitrophenyl myristate Malbranchea cinnamomea 4-nitrophenol + decanoate
-
 ?
4-nitrophenyl myristate + H2O
-
 Malbranchea cinnamomea 4-nitrophenol + myristate
-
 ?
4-nitrophenyl palmitate + H2O 54% of the activity with -nitrophenyl myristate Malbranchea cinnamomea 4-nitrophenol + palmitate
-
 ?
glyceryl trioleate + H2O
-
 Malbranchea cinnamomea glycerol 1,2-dioleate + oleate
-
 ?
additional information enzyme is active toward 4-nitrophenyl esters of various carbon chain lengths with peak activity on long-chain fatty acid (C14). It displays high sn-1,3-regioselectivity on hydrolyzing triolein Malbranchea cinnamomea ?
-
 ?
oleate + methanol
-
 Malbranchea cinnamomea oleic acid methyl ester
-
 ?

Synonyms

Synonyms Comment Organism
LipA
-
 Malbranchea cinnamomea

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
-
 Malbranchea cinnamomea

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 24 h, more than 93% residual activity Malbranchea cinnamomea

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
-
 Malbranchea cinnamomea