Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Pyrococcus furiosus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pyrococcus furiosus | Q8TZJ1 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Pyrococcus furiosus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl butyrate + H2O | - |
Pyrococcus furiosus | 4-nitrophenol + butyrate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Pf2001DELTA60 | - |
Pyrococcus furiosus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
66 | - |
enzyme immobilized on octyl-agarose or glyoxyl agarose | Pyrococcus furiosus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
66 | 90 | 66°C: optimum, 90°C: enzyme immobilized on glyoxyl agaroseis active at 90°C | Pyrococcus furiosus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
thermostability of the enzyme immobilized on different supports. The highest enzyme stability after 48 h incubation at 70°C is achieved with immobilization on glyoxylagarose (82%). The enzyme immobilized on this support in the presence of DTT at pH 7 maintains around 69% activity. The immobilization of the enzyme on octyl-agarose (interfacially activated), yields the worst lipase biocatalyst (27% remaining activity) | Pyrococcus furiosus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
enzyme immobilized on octyl-agarose or glyoxyl agarose | Pyrococcus furiosus |