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Literature summary for 3.1.1.3 extracted from
- Characterization of an extracellular lipase and its chaperone from Ralstonia eutropha H16 (2013), Appl. Microbiol. Biotechnol., 97, 2443-2454.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| Triton X-100 | 586% activity at 0.001% (w/v) and 333% activity at 0.1% (w/v) | Cupriavidus necator |  |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Cu2+ | 11% residual activity at 1 mM | Cupriavidus necator | |
| EDTA | 15% residual activity at 1 mM | Cupriavidus necator | |
| EGTA | 15% residual activity at 1 mM | Cupriavidus necator | |
| Fe2+ | 18% residual activity at 1 mM | Cupriavidus necator | |
| Ni2+ | 7% residual activity at 1 mM | Cupriavidus necator | |
| SDS | 10% residual activity at 0.001% (v/v) | Cupriavidus necator | |
| Tween 40 | 10% residual activity at 0.001% (v/v) | Cupriavidus necator | |
| Tween 60 | 10% residual activity at 0.001% (v/v) | Cupriavidus necator | |
| Tween 80 | 10% residual activity at 0.001% (v/v) | Cupriavidus necator | |
| Zn2+ | 3% residual activity at 1 mM | Cupriavidus necator | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Cupriavidus necator | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | activates at 0.1 mM | Cupriavidus necator | |
| Mg2+ | activates at 0.1 mM | Cupriavidus necator | |
| Mn2+ | 115% activity at 0.1 mM | Cupriavidus necator | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 38600 | - |
x * 38600, calculated from amino acid sequence | Cupriavidus necator |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Cupriavidus necator | - |
- |
- |
| Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1 | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl palmitate + H2O | - |
Cupriavidus necator | 4-nitrophenol + palmitate | - |
? | |
| 4-nitrophenyl palmitate + H2O | - |
Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1 | 4-nitrophenol + palmitate | - |
? | |
| additional information | LipA is a non-specific lipase able to act at random for the complete breakdown of triacylglycerols | Cupriavidus necator | ? | - |
? | |
| additional information | LipA is a non-specific lipase able to act at random for the complete breakdown of triacylglycerols | Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 38600, calculated from amino acid sequence | Cupriavidus necator |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| LipA | - |
Cupriavidus necator |
| lipase | - |
Cupriavidus necator |
| triacylglycerol acylhydrolase | - |
Cupriavidus necator |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
- |
Cupriavidus necator |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 10 | 70 | - |
Cupriavidus necator |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 8 | the enzyme is most active between pH 7.0 and 8.0 at room temperature | Cupriavidus necator |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| additional information | cofactors are not required for lipase activity | Cupriavidus necator |
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