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Literature summary for 3.1.1.3 extracted from

  • Cho, S.S.; Park, D.J.; Simkhada, J.R.; Hong, J.H.; Sohng, J.K.; Lee, O.H.; Yoo, J.C.
    A neutral lipase applicable in biodiesel production from a newly isolated Streptomyces sp. CS326 (2012), Bioprocess Biosyst. Eng., 35, :227-234.
    View publication on PubMed

Application

Application Comment Organism
biofuel production LP326 catalyzes biodiesel production using methanol and various oils Streptomyces sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.24
-
4-nitrophenyl palmitate pH 7.0, 40°C Streptomyces sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
17000
-
x * 17000, SDS-PAGE Streptomyces sp.

Organism

Organism UniProt Comment Textmining
Streptomyces sp.
-
-
-
Streptomyces sp. CS326
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Sepharose CL-6B column chromatography Streptomyces sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl palmitate + H2O preferred substrate Streptomyces sp. 4-nitrophenol + palmitate
-
?
4-nitrophenyl palmitate + H2O preferred substrate Streptomyces sp. CS326 4-nitrophenol + palmitate
-
?

Subunits

Subunits Comment Organism
? x * 17000, SDS-PAGE Streptomyces sp.

Synonyms

Synonyms Comment Organism
lipase
-
Streptomyces sp.
LP326
-
Streptomyces sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
Streptomyces sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
the enzyme is stable below 50°C Streptomyces sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
Streptomyces sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
5 8
-
Streptomyces sp.