Activating Compound | Comment | Organism | Structure |
---|---|---|---|
PEG 20000 | 124% activity at 0.1% (q/v) | Aspergillus fumigatus | |
polyvinyl alcohol | 118% activity at 0.1% (w/v) | Aspergillus fumigatus | |
Triton X-100 | 128% activity at 0.1% (v/v) | Aspergillus fumigatus |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Aspergillus fumigatus |
Protein Variants | Comment | Organism |
---|---|---|
D260A | the mutant shows reduced activity compared to the wild type enzyme | Aspergillus fumigatus |
D264A | the mutant shows reduced activity compared to the wild type enzyme | Aspergillus fumigatus |
H283A | the mutant shows reduced activity compared to the wild type enzyme | Aspergillus fumigatus |
H290A | the mutant shows reduced activity compared to the wild type enzyme | Aspergillus fumigatus |
S165A | the mutant shows reduced activity compared to the wild type enzyme | Aspergillus fumigatus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | 47.5% residual activity at 2 mM | Aspergillus fumigatus | |
Fe2+ | 57.2% residual activity at 2 mM | Aspergillus fumigatus | |
Li+ | 76.4% residual activity at 1 mM | Aspergillus fumigatus | |
Mg2+ | 85% residual activity at 2 mM | Aspergillus fumigatus | |
additional information | not inhibited by Ca2+, Mn2+, Ni2+, Ba2+, and EDTA | Aspergillus fumigatus | |
SDS | 0.201% residual activity at 0.1% (w/v) | Aspergillus fumigatus | |
Zn2+ | 32.2% residual activity at 2 mM | Aspergillus fumigatus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
14 | - |
4-nitrophenyl acetate | at 30°C, pH 7.5 | Aspergillus fumigatus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | metal ions cannot activate the lipase | Aspergillus fumigatus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
38000 | - |
x * 38000, SDS-PAGE | Aspergillus fumigatus |
Organic Solvent | Comment | Organism |
---|---|---|
1-butanol | 0.917% residual activity at 50% (v/v), 4.28% residual activity at 10% (v/v) | Aspergillus fumigatus |
Acetone | 0.917% residual activity at 50% (v/v), 113% activity at 10% (v/v) | Aspergillus fumigatus |
DMSO | 57.2% residual activity at 50% (v/v), 99.1% activity at 10% (v/v) | Aspergillus fumigatus |
Ethanol | 2.14% residual activity at 50% (v/v), 100% activity at 10% (v/v) | Aspergillus fumigatus |
isopropanol | no activity at 50% (v/v), 83.8% activity at 10% (v/v) | Aspergillus fumigatus |
Methanol | 0.613% residual activity at 50% (v/v), 108% activity at 10% (v/v) | Aspergillus fumigatus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus fumigatus | - |
- |
- |
Aspergillus fumigatus CGMCC 2873 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA agarose column chromatography | Aspergillus fumigatus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
mycelium | - |
Aspergillus fumigatus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
118 | - |
supernatant of cell lysate, using 4-nitrophenyl acetate as substrate, at 30°C, pH 7.5 | Aspergillus fumigatus |
1000 | - |
after 8.47fold purification, using 4-nitrophenyl acetate as substrate, at 30°C, pH 7.5 | Aspergillus fumigatus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl acetate + H2O | 100% activity | Aspergillus fumigatus | 4-nitrophenol + acetate | - |
? | |
4-nitrophenyl acetate + H2O | 100% activity | Aspergillus fumigatus CGMCC 2873 | 4-nitrophenol + acetate | - |
? | |
4-nitrophenyl butyrate + H2O | about 65% activity compared to 4-nitrophenyl acetate | Aspergillus fumigatus | 4-nitrophenol + butyrate | - |
? | |
4-nitrophenyl butyrate + H2O | about 65% activity compared to 4-nitrophenyl acetate | Aspergillus fumigatus CGMCC 2873 | 4-nitrophenol + butyrate | - |
? | |
4-nitrophenyl decanoate + H2O | about 10% activity compared to 4-nitrophenyl acetate | Aspergillus fumigatus | 4-nitrophenol + decanoate | - |
? | |
4-nitrophenyl laurate + H2O | - |
Aspergillus fumigatus | 4-nitrophenol + laurate | - |
? | |
4-nitrophenyl myristate + H2O | worst substrate, less than 5% activity compared to 4-nitrophenyl acetate | Aspergillus fumigatus | 4-nitrophenol + myristate | - |
? | |
4-nitrophenyl propionate + H2O | about 60% activity compared to 4-nitrophenyl acetate | Aspergillus fumigatus | 4-nitrophenol + propionate | - |
? | |
4-nitrophenyl propionate + H2O | about 60% activity compared to 4-nitrophenyl acetate | Aspergillus fumigatus CGMCC 2873 | 4-nitrophenol + propionate | - |
? | |
additional information | AFL1-1 has potential enantioselectivity for the ability to catalyze methyl 3-(4-methoxyphenyl) glycidate and methyl 3-phenylglycidate | Aspergillus fumigatus | ? | - |
? | |
additional information | almost no activity on long-chained acyl esters such as 4-nitrophenyl palmitate | Aspergillus fumigatus | ? | - |
? | |
additional information | AFL1-1 has potential enantioselectivity for the ability to catalyze methyl 3-(4-methoxyphenyl) glycidate and methyl 3-phenylglycidate | Aspergillus fumigatus CGMCC 2873 | ? | - |
? | |
additional information | almost no activity on long-chained acyl esters such as 4-nitrophenyl palmitate | Aspergillus fumigatus CGMCC 2873 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 38000, SDS-PAGE | Aspergillus fumigatus |
Synonyms | Comment | Organism |
---|---|---|
AFL1-1 | recombinant enzyme | Aspergillus fumigatus |
lipase | - |
Aspergillus fumigatus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | - |
above the optimal temperature, the activity decreases sharply with almost 80% loss of its original activity at 75°C | Aspergillus fumigatus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 50 | the enzyme is stable (60% activity) at 30-50 °C for 1 h | Aspergillus fumigatus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
- |
Aspergillus fumigatus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
3 | 10 | at pH 7.0, the activity remains at nearly 100% of the maximal activity, and drops dramatically at pH below 9.0 | Aspergillus fumigatus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6 | 8.5 | the enzyme is stable in the pH range 6.0-8.5 for 16 h (at 4 °C) | Aspergillus fumigatus |