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Literature summary for 3.1.1.3 extracted from

  • Wang, Y.; Zhao, J.; Xu, J.H.; Fan, L.Q.; Li, S.X.; Zhao, L.L.; Mao, X.B.
    Significantly improved expression and biochemical properties of recombinant Serratia marcescens lipase as robust biocatalyst for kinetic resolution of chiral ester (2010), Appl. Biochem. Biotechnol., 162, 2387-2399.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
additional information the enzyme is not influenced by PEG1500 and PEG20000 Serratia marcescens
PEG4000 116% activity at 0.1% (w/v) Serratia marcescens

Application

Application Comment Organism
synthesis the highly enantioselective lipase from Serratia marcescens ECU1010 is a robust biocatalyst for practical use in large-scale production of diltiazem intermediate Serratia marcescens

Cloned(Commentary)

Cloned (Comment) Organism
expressed as an N-terminus His-tag fusion protein in Escherichia coli BL21(DE3) cells Serratia marcescens

Inhibitors

Inhibitors Comment Organism Structure
EDTA 25.3% residual activity at 2 mM Serratia marcescens
SDS 20.1% residual activity at 0.1% (w/v) Serratia marcescens
Triton X-100 2.3% residual activity at 0.1% (w/v) Serratia marcescens
Tween 20 80.1% residual activity at 0.1% (w/v) Serratia marcescens
Zn2+ 80% residual activity at 2 mM Serratia marcescens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
222
-
(+/-)-trans-3-(4'-methoxy-phenyl)-glycidyl methyl ester at pH 7.5 and 30°C Serratia marcescens

Metals/Ions

Metals/Ions Comment Organism Structure
Ba2+ 130% activity at 2 mM Serratia marcescens
Ca2+ adding Ca2+ to a final concentration of 5 mM increases the activity of lipase by nearly 5fold with respect to the control Serratia marcescens
Cu2+ 172.5% activity at 2 mM Serratia marcescens
Li+ 140% activity at 2 mM Serratia marcescens
additional information the enzyme is not influenced by Fe2+, Mn2+, and Mg2+ Serratia marcescens
Ni2+ 172.5% activity at 2 mM Serratia marcescens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
65000
-
x * 65000, SDS-PAGE Serratia marcescens

Organism

Organism UniProt Comment Textmining
Serratia marcescens
-
-
-
Serratia marcescens ECU1010
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA agarose column chromatography Serratia marcescens

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4
-
enzyme from supernatant of cell lysate, using 4-nitrophenyl acetate as substrate, at pH 7.5 and 30°C Serratia marcescens
5.7
-
enzyme after 2.4fold purification, using 4-nitrophenyl acetate as substrate, at pH 7.5 and 30°C Serratia marcescens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(+/-)-trans-3-(4'-methoxy-phenyl)-glycidyl methyl ester + H2O 50% conversion yield after 4 h in 100 mM potassium phosphate buffer, pH 7.5, at 30 °C Serratia marcescens (2R,3S)-3-(4-hydroxyphenyl)oxirane-2-carboxylic acid + methyl (2S,3R)-3-(4-hydroxyphenyl)oxirane-2-carboxylate + methanol
-
?
4-nitrophenyl acetate + H2O worst substrate, less than 5% activity compared to 4-nitrophenyl laurate Serratia marcescens 4-nitrophenol + acetate
-
?
4-nitrophenyl acetate + H2O worst substrate, less than 5% activity compared to 4-nitrophenyl laurate Serratia marcescens ECU1010 4-nitrophenol + acetate
-
?
4-nitrophenyl butyrate + H2O about 10% activity compared to 4-nitrophenyl laurate Serratia marcescens 4-nitrophenol + butyrate
-
?
4-nitrophenyl butyrate + H2O about 10% activity compared to 4-nitrophenyl laurate Serratia marcescens ECU1010 4-nitrophenol + butyrate
-
?
4-nitrophenyl decanoate + H2O about 30% activity compared to 4-nitrophenyl laurate Serratia marcescens 4-nitrophenol + decanoate
-
?
4-nitrophenyl decanoate + H2O about 30% activity compared to 4-nitrophenyl laurate Serratia marcescens ECU1010 4-nitrophenol + decanoate
-
?
4-nitrophenyl laurate + H2O maximum activity on 4-nitrophenyl laurate Serratia marcescens 4-nitrophenol + laurate
-
?
4-nitrophenyl myristate + H2O about 90% activity compared to 4-nitrophenyl laurate Serratia marcescens 4-nitrophenol + myristate
-
?
4-nitrophenyl palmitate + H2O about 30% activity compared to 4-nitrophenyl laurate Serratia marcescens 4-nitrophenol + palmitate
-
?
4-nitrophenyl palmitate + H2O about 30% activity compared to 4-nitrophenyl laurate Serratia marcescens ECU1010 4-nitrophenol + palmitate
-
?
4-nitrophenyl propionate + H2O about 5% activity compared to 4-nitrophenyl laurate Serratia marcescens 4-nitrophenol + propionate
-
?
4-nitrophenyl propionate + H2O about 5% activity compared to 4-nitrophenyl laurate Serratia marcescens ECU1010 4-nitrophenol + propionate
-
?

Subunits

Subunits Comment Organism
? x * 65000, SDS-PAGE Serratia marcescens

Synonyms

Synonyms Comment Organism
lipase
-
Serratia marcescens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
Serratia marcescens

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
25 45 the purified lipase keeps not less than 80% of the maximum activity at 40°C in a temperature range of 25-45°C Serratia marcescens

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30 50 the recombinant lipase displays excellent stability below 30°C. After being incubated at temperatures of 30, 40, and 50°Cfor 0.5-2 h, the enzyme has a remaining residual activity of over 90% at 30°C compared with the initial activity before incubation. However, 0.5 h later, the residual activity decreases sharply when the temperature is above 50°C Serratia marcescens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
-
Serratia marcescens

pH Stability

pH Stability pH Stability Maximum Comment Organism
5 6.8 the recombinant lipase displays excellent stability at pH 5.0-6.8 and is stable over a pH range of 5.0-6.8 with more than 80% residual activity after being incubated at 4°C for 20 h Serratia marcescens