Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | the enzyme is not influenced by PEG1500 and PEG20000 | Serratia marcescens | |
PEG4000 | 116% activity at 0.1% (w/v) | Serratia marcescens |
Application | Comment | Organism |
---|---|---|
synthesis | the highly enantioselective lipase from Serratia marcescens ECU1010 is a robust biocatalyst for practical use in large-scale production of diltiazem intermediate | Serratia marcescens |
Cloned (Comment) | Organism |
---|---|
expressed as an N-terminus His-tag fusion protein in Escherichia coli BL21(DE3) cells | Serratia marcescens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | 25.3% residual activity at 2 mM | Serratia marcescens | |
SDS | 20.1% residual activity at 0.1% (w/v) | Serratia marcescens | |
Triton X-100 | 2.3% residual activity at 0.1% (w/v) | Serratia marcescens | |
Tween 20 | 80.1% residual activity at 0.1% (w/v) | Serratia marcescens | |
Zn2+ | 80% residual activity at 2 mM | Serratia marcescens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
222 | - |
(+/-)-trans-3-(4'-methoxy-phenyl)-glycidyl methyl ester | at pH 7.5 and 30°C | Serratia marcescens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ba2+ | 130% activity at 2 mM | Serratia marcescens | |
Ca2+ | adding Ca2+ to a final concentration of 5 mM increases the activity of lipase by nearly 5fold with respect to the control | Serratia marcescens | |
Cu2+ | 172.5% activity at 2 mM | Serratia marcescens | |
Li+ | 140% activity at 2 mM | Serratia marcescens | |
additional information | the enzyme is not influenced by Fe2+, Mn2+, and Mg2+ | Serratia marcescens | |
Ni2+ | 172.5% activity at 2 mM | Serratia marcescens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
65000 | - |
x * 65000, SDS-PAGE | Serratia marcescens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Serratia marcescens | - |
- |
- |
Serratia marcescens ECU1010 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA agarose column chromatography | Serratia marcescens |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
2.4 | - |
enzyme from supernatant of cell lysate, using 4-nitrophenyl acetate as substrate, at pH 7.5 and 30°C | Serratia marcescens |
5.7 | - |
enzyme after 2.4fold purification, using 4-nitrophenyl acetate as substrate, at pH 7.5 and 30°C | Serratia marcescens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(+/-)-trans-3-(4'-methoxy-phenyl)-glycidyl methyl ester + H2O | 50% conversion yield after 4 h in 100 mM potassium phosphate buffer, pH 7.5, at 30 °C | Serratia marcescens | (2R,3S)-3-(4-hydroxyphenyl)oxirane-2-carboxylic acid + methyl (2S,3R)-3-(4-hydroxyphenyl)oxirane-2-carboxylate + methanol | - |
? | |
4-nitrophenyl acetate + H2O | worst substrate, less than 5% activity compared to 4-nitrophenyl laurate | Serratia marcescens | 4-nitrophenol + acetate | - |
? | |
4-nitrophenyl acetate + H2O | worst substrate, less than 5% activity compared to 4-nitrophenyl laurate | Serratia marcescens ECU1010 | 4-nitrophenol + acetate | - |
? | |
4-nitrophenyl butyrate + H2O | about 10% activity compared to 4-nitrophenyl laurate | Serratia marcescens | 4-nitrophenol + butyrate | - |
? | |
4-nitrophenyl butyrate + H2O | about 10% activity compared to 4-nitrophenyl laurate | Serratia marcescens ECU1010 | 4-nitrophenol + butyrate | - |
? | |
4-nitrophenyl decanoate + H2O | about 30% activity compared to 4-nitrophenyl laurate | Serratia marcescens | 4-nitrophenol + decanoate | - |
? | |
4-nitrophenyl decanoate + H2O | about 30% activity compared to 4-nitrophenyl laurate | Serratia marcescens ECU1010 | 4-nitrophenol + decanoate | - |
? | |
4-nitrophenyl laurate + H2O | maximum activity on 4-nitrophenyl laurate | Serratia marcescens | 4-nitrophenol + laurate | - |
? | |
4-nitrophenyl myristate + H2O | about 90% activity compared to 4-nitrophenyl laurate | Serratia marcescens | 4-nitrophenol + myristate | - |
? | |
4-nitrophenyl palmitate + H2O | about 30% activity compared to 4-nitrophenyl laurate | Serratia marcescens | 4-nitrophenol + palmitate | - |
? | |
4-nitrophenyl palmitate + H2O | about 30% activity compared to 4-nitrophenyl laurate | Serratia marcescens ECU1010 | 4-nitrophenol + palmitate | - |
? | |
4-nitrophenyl propionate + H2O | about 5% activity compared to 4-nitrophenyl laurate | Serratia marcescens | 4-nitrophenol + propionate | - |
? | |
4-nitrophenyl propionate + H2O | about 5% activity compared to 4-nitrophenyl laurate | Serratia marcescens ECU1010 | 4-nitrophenol + propionate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 65000, SDS-PAGE | Serratia marcescens |
Synonyms | Comment | Organism |
---|---|---|
lipase | - |
Serratia marcescens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
- |
Serratia marcescens |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 45 | the purified lipase keeps not less than 80% of the maximum activity at 40°C in a temperature range of 25-45°C | Serratia marcescens |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 50 | the recombinant lipase displays excellent stability below 30°C. After being incubated at temperatures of 30, 40, and 50°Cfor 0.5-2 h, the enzyme has a remaining residual activity of over 90% at 30°C compared with the initial activity before incubation. However, 0.5 h later, the residual activity decreases sharply when the temperature is above 50°C | Serratia marcescens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
- |
Serratia marcescens |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5 | 6.8 | the recombinant lipase displays excellent stability at pH 5.0-6.8 and is stable over a pH range of 5.0-6.8 with more than 80% residual activity after being incubated at 4°C for 20 h | Serratia marcescens |