Activating Compound | Comment | Organism | Structure |
---|---|---|---|
phenylmethylsulfonyl fluoride | 163% activity at 1 mM | Acinetobacter baylyi |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ag+ | 56% residual activity at 1 mM | Acinetobacter baylyi | |
Ba2+ | 69% residual activity at 1 mM | Acinetobacter baylyi | |
dithiothreitol | 86% residual activity at 1 mM | Acinetobacter baylyi | |
EDTA | complete inhibition at 1 mM | Acinetobacter baylyi | |
Fe2+ | complete inhibition at 1 mM | Acinetobacter baylyi | |
Hg2+ | 77% residual activity at 1 mM | Acinetobacter baylyi | |
K+ | 39% residual activity at 1 mM | Acinetobacter baylyi | |
Mn2+ | complete inhibition at 1 mM | Acinetobacter baylyi | |
Ni2+ | 60% residual activity at 1 mM | Acinetobacter baylyi | |
SDS | complete inhibition at 1% (w/v) | Acinetobacter baylyi | |
Triton X-100 | complete inhibition at 1% (w/v) | Acinetobacter baylyi | |
Tween 80 | 4% residual activity at 1% (v/v) | Acinetobacter baylyi | |
Zn2+ | 67% residual activity at 1 mM | Acinetobacter baylyi |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | 265% activity at 1 mM | Acinetobacter baylyi | |
additional information | no significant effect is found in the presence of Ca2+ and Li+ | Acinetobacter baylyi | |
Na+ | 214% activity at 1 mM | Acinetobacter baylyi |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
30000 | - |
x * 30000, SDS-PAGE | Acinetobacter baylyi |
Organic Solvent | Comment | Organism |
---|---|---|
acetonitrile | 66% activity at 50% (v/v) | Acinetobacter baylyi |
benzene | the enzyme is highly resistant to 50% (v/v) benzene | Acinetobacter baylyi |
Butanol | 60% activity at 50% (v/v) | Acinetobacter baylyi |
DMSO | 92% activity at 50% (v/v) | Acinetobacter baylyi |
Ethanol | 60% activity at 50% (v/v) | Acinetobacter baylyi |
hexane | 72% activity at 50% (v/v) | Acinetobacter baylyi |
isoamyl alcohol | the enzyme is highly resistant to 50% (v/v) isoamylalcohol (108% activity) | Acinetobacter baylyi |
isopropanol | 59% activity at 50% (v/v) | Acinetobacter baylyi |
Methanol | 64% activity at 50% (v/v) | Acinetobacter baylyi |
n-decane | 75% activity at 50% (v/v) | Acinetobacter baylyi |
n-heptane | 92% activity at 50% (v/v) | Acinetobacter baylyi |
n-hexadecane | 81% activity at 50% (v/v) | Acinetobacter baylyi |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acinetobacter baylyi | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation and Sephadex G-75 gel filtration | Acinetobacter baylyi |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
5.62 | - |
cell-free crude extract, at pH 8.0 and 60°C | Acinetobacter baylyi |
123 | - |
after 21.89fold purification, at pH 8.0 and 60°C | Acinetobacter baylyi |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl acetate + H2O | about 30% activity compared to 4-nitrophenyl caprate | Acinetobacter baylyi | 4-nitrophenol + acetate | - |
? | |
4-nitrophenyl butyrate + H2O | about 65% activity compared to 4-nitrophenyl caprate | Acinetobacter baylyi | 4-nitrophenol + butyrate | - |
? | |
4-nitrophenyl caprate + H2O | best substrate | Acinetobacter baylyi | 4-nitrophenol + caprate | - |
? | |
4-nitrophenyl caprylate + H2O | about 85% activity compared to 4-nitrophenyl caprate | Acinetobacter baylyi | 4-nitrophenol + caprylate | - |
? | |
4-nitrophenyl laurate + H2O | about 90% activity compared to 4-nitrophenyl caprate | Acinetobacter baylyi | 4-nitrophenol + laurate | - |
? | |
4-nitrophenyl myristate + H2O | about 50% activity compared to 4-nitrophenyl caprate | Acinetobacter baylyi | 4-nitrophenol + myristate | - |
? | |
4-nitrophenyl palmitate + H2O | about 40% activity compared to 4-nitrophenyl caprate | Acinetobacter baylyi | 4-nitrophenol + palmitate | - |
? | |
4-nitrophenyl stearate + H2O | about 35% activity compared to 4-nitrophenyl caprate | Acinetobacter baylyi | 4-nitrophenol + stearate | - |
? | |
additional information | the enzyme 11folds favorably catalyzes the hydrolysis of rice bran oil, sesame oil, and coconut oil in comparison to palm oil. The transesterification activity of palm oil to fatty acid methyl esters reveals 31.64% after 48h. Wasting palm oil shows the highest activity among all oil substrates tested (about 1300% activity compared to palm oil). COmpared to palm oil, the enzyme shows about 150% activity with sunflower oil, about 150% activity with safflower oil, about 300% activity with canola oil, about 300% activity with olive oil, about 100% activity with soybean oil, and about 600% activity with camellia tea oil | Acinetobacter baylyi | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 30000, SDS-PAGE | Acinetobacter baylyi |
Synonyms | Comment | Organism |
---|---|---|
lipase | - |
Acinetobacter baylyi |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
- |
Acinetobacter baylyi |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 100 | the lipase activity is raised gradually from 20 to 50°C and then increases steeply and reaches to the maximum at 60°C as well as decreases steeply after maximum. From 70 to 90°C, the lipase activities reduce very slightly and it loses all activity at 100°C | Acinetobacter baylyi |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | 80 | the enzyme retains more than 80% of initial activity after 6 h at 60-80°C | Acinetobacter baylyi |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Acinetobacter baylyi |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 10 | the relative activity increases gradually from pH 5.0 to 7.0. The residual activity increases steeply and reaches the maximum at pH 8.0 as well as decreases steeply after maximum. No lipase activity is observed after pH 10.0 | Acinetobacter baylyi |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6 | 9 | - |
Acinetobacter baylyi |