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Literature summary for 3.1.1.3 extracted from

  • Uttatree, S.; Winayanuwattikun, P.; Charoenpanich, J.
    Isolation and characterization of a novel thermophilic-organic solvent stable lipase from Acinetobacter baylyi (2010), Appl. Biochem. Biotechnol., 162, 1362-1376.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
phenylmethylsulfonyl fluoride 163% activity at 1 mM Acinetobacter baylyi

Inhibitors

Inhibitors Comment Organism Structure
Ag+ 56% residual activity at 1 mM Acinetobacter baylyi
Ba2+ 69% residual activity at 1 mM Acinetobacter baylyi
dithiothreitol 86% residual activity at 1 mM Acinetobacter baylyi
EDTA complete inhibition at 1 mM Acinetobacter baylyi
Fe2+ complete inhibition at 1 mM Acinetobacter baylyi
Hg2+ 77% residual activity at 1 mM Acinetobacter baylyi
K+ 39% residual activity at 1 mM Acinetobacter baylyi
Mn2+ complete inhibition at 1 mM Acinetobacter baylyi
Ni2+ 60% residual activity at 1 mM Acinetobacter baylyi
SDS complete inhibition at 1% (w/v) Acinetobacter baylyi
Triton X-100 complete inhibition at 1% (w/v) Acinetobacter baylyi
Tween 80 4% residual activity at 1% (v/v) Acinetobacter baylyi
Zn2+ 67% residual activity at 1 mM Acinetobacter baylyi

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ 265% activity at 1 mM Acinetobacter baylyi
additional information no significant effect is found in the presence of Ca2+ and Li+ Acinetobacter baylyi
Na+ 214% activity at 1 mM Acinetobacter baylyi

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
30000
-
x * 30000, SDS-PAGE Acinetobacter baylyi

Organic Solvent Stability

Organic Solvent Comment Organism
acetonitrile 66% activity at 50% (v/v) Acinetobacter baylyi
benzene the enzyme is highly resistant to 50% (v/v) benzene Acinetobacter baylyi
Butanol 60% activity at 50% (v/v) Acinetobacter baylyi
DMSO 92% activity at 50% (v/v) Acinetobacter baylyi
Ethanol 60% activity at 50% (v/v) Acinetobacter baylyi
hexane 72% activity at 50% (v/v) Acinetobacter baylyi
isoamyl alcohol the enzyme is highly resistant to 50% (v/v) isoamylalcohol (108% activity) Acinetobacter baylyi
isopropanol 59% activity at 50% (v/v) Acinetobacter baylyi
Methanol 64% activity at 50% (v/v) Acinetobacter baylyi
n-decane 75% activity at 50% (v/v) Acinetobacter baylyi
n-heptane 92% activity at 50% (v/v) Acinetobacter baylyi
n-hexadecane 81% activity at 50% (v/v) Acinetobacter baylyi

Organism

Organism UniProt Comment Textmining
Acinetobacter baylyi
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation and Sephadex G-75 gel filtration Acinetobacter baylyi

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5.62
-
cell-free crude extract, at pH 8.0 and 60°C Acinetobacter baylyi
123
-
after 21.89fold purification, at pH 8.0 and 60°C Acinetobacter baylyi

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl acetate + H2O about 30% activity compared to 4-nitrophenyl caprate Acinetobacter baylyi 4-nitrophenol + acetate
-
?
4-nitrophenyl butyrate + H2O about 65% activity compared to 4-nitrophenyl caprate Acinetobacter baylyi 4-nitrophenol + butyrate
-
?
4-nitrophenyl caprate + H2O best substrate Acinetobacter baylyi 4-nitrophenol + caprate
-
?
4-nitrophenyl caprylate + H2O about 85% activity compared to 4-nitrophenyl caprate Acinetobacter baylyi 4-nitrophenol + caprylate
-
?
4-nitrophenyl laurate + H2O about 90% activity compared to 4-nitrophenyl caprate Acinetobacter baylyi 4-nitrophenol + laurate
-
?
4-nitrophenyl myristate + H2O about 50% activity compared to 4-nitrophenyl caprate Acinetobacter baylyi 4-nitrophenol + myristate
-
?
4-nitrophenyl palmitate + H2O about 40% activity compared to 4-nitrophenyl caprate Acinetobacter baylyi 4-nitrophenol + palmitate
-
?
4-nitrophenyl stearate + H2O about 35% activity compared to 4-nitrophenyl caprate Acinetobacter baylyi 4-nitrophenol + stearate
-
?
additional information the enzyme 11folds favorably catalyzes the hydrolysis of rice bran oil, sesame oil, and coconut oil in comparison to palm oil. The transesterification activity of palm oil to fatty acid methyl esters reveals 31.64% after 48h. Wasting palm oil shows the highest activity among all oil substrates tested (about 1300% activity compared to palm oil). COmpared to palm oil, the enzyme shows about 150% activity with sunflower oil, about 150% activity with safflower oil, about 300% activity with canola oil, about 300% activity with olive oil, about 100% activity with soybean oil, and about 600% activity with camellia tea oil Acinetobacter baylyi ?
-
?

Subunits

Subunits Comment Organism
? x * 30000, SDS-PAGE Acinetobacter baylyi

Synonyms

Synonyms Comment Organism
lipase
-
Acinetobacter baylyi

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
Acinetobacter baylyi

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 100 the lipase activity is raised gradually from 20 to 50°C and then increases steeply and reaches to the maximum at 60°C as well as decreases steeply after maximum. From 70 to 90°C, the lipase activities reduce very slightly and it loses all activity at 100°C Acinetobacter baylyi

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60 80 the enzyme retains more than 80% of initial activity after 6 h at 60-80°C Acinetobacter baylyi

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
Acinetobacter baylyi

pH Range

pH Minimum pH Maximum Comment Organism
5 10 the relative activity increases gradually from pH 5.0 to 7.0. The residual activity increases steeply and reaches the maximum at pH 8.0 as well as decreases steeply after maximum. No lipase activity is observed after pH 10.0 Acinetobacter baylyi

pH Stability

pH Stability pH Stability Maximum Comment Organism
6 9
-
Acinetobacter baylyi