Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Fe3+ | 132% relative activity after 30 min at 1 mM | Pseudomonas aeruginosa | |
glutathione | 112% relative activity after 30 min at 1 mM | Pseudomonas aeruginosa |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | reduces the enzyme activity to 48% after 1 h | Pseudomonas aeruginosa | |
cetyltrimethylammonium bromide | 45% residual activity after 5 h at 25% (v/v) | Pseudomonas aeruginosa | |
CHAPS | 86% residual activity after 5 h at 25% (v/v) | Pseudomonas aeruginosa | |
Cu2+ | 70% residual activity after 30 min at 8 mM | Pseudomonas aeruginosa | |
diisopropylfluorophosphate | 82% residual activity after 30 min at 8 mM | Pseudomonas aeruginosa | |
hydrogen peroxide | 62% residual activity after 5 h at 25% (v/v) | Pseudomonas aeruginosa | |
additional information | the enzyme activity is not affected significantly by Hg2+, Ca2+, Mg2+, Fe3+, EDTA and phenylmethylsulfonyl fluoride (1 mM each) | Pseudomonas aeruginosa | |
p-chloromercuric benzoate | 20% residual activity after 30 min at 8 mM | Pseudomonas aeruginosa | |
sodium deoxycholate | 90% residual activity after 5 h at 25% (v/v) | Pseudomonas aeruginosa | |
sodium hypochlorite | 85% residual activity after 5 h at 25% (v/v) | Pseudomonas aeruginosa | |
Tween 20 | 74% residual activity after 5 h at 25% (v/v) | Pseudomonas aeruginosa | |
Zn2+ | 51% residual activity after 30 min at 1 mM | Pseudomonas aeruginosa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
70.4 | - |
4-nitrophenyl palmitate | in 0.1 M Tris-HCl buffer (pH 8.0), at 40°C | Pseudomonas aeruginosa |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
60000 | - |
SDS-PAGE | Pseudomonas aeruginosa |
Organic Solvent | Comment | Organism |
---|---|---|
Brij 35 | 121% relative activity after 5 h at 25% (v/v) | Pseudomonas aeruginosa |
additional information | the enzyme activity is not significantly affected by dimethylsulfonyloxide, isopropanol, ethanol, methanol, benzene, toluene, xylene, cyclohexane, hexane, n-heptane, isooctane, decane, and tetradecane | Pseudomonas aeruginosa |
Tween 80 | 124% relative activity after 5 h at 25% (v/v) | Pseudomonas aeruginosa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas aeruginosa | - |
- |
- |
Pseudomonas aeruginosa PseA | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ultrafiltration and Sephadex G-100 gel filtration | Pseudomonas aeruginosa |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
16.6 | - |
crude filtrate, in Tris-HCl, buffer (0.1 M, pH 8.0) | Pseudomonas aeruginosa |
143.3 | - |
after 8.6fold purification, in Tris-HCl, buffer (0.1 M, pH 8.0) | Pseudomonas aeruginosa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3 triolein + 3 H2O | - |
Pseudomonas aeruginosa | 1,3-diolein + 1,2-diolein + 2,3-diolein + 3 oleate | shows random positional specificity for triolein hydrolysis | ? | |
4-nitrophenyl acetate + H2O | - |
Pseudomonas aeruginosa | 4-nitrophenol + acetate | - |
? | |
4-nitrophenyl acetate + H2O | - |
Pseudomonas aeruginosa PseA | 4-nitrophenol + acetate | - |
? | |
4-nitrophenyl butyrate + H2O | - |
Pseudomonas aeruginosa | 4-nitrophenol + butyrate | - |
? | |
4-nitrophenyl butyrate + H2O | - |
Pseudomonas aeruginosa PseA | 4-nitrophenol + butyrate | - |
? | |
4-nitrophenyl caprate + H2O | - |
Pseudomonas aeruginosa | 4-nitrophenol + caprate | - |
? | |
4-nitrophenyl caprate + H2O | - |
Pseudomonas aeruginosa PseA | 4-nitrophenol + caprate | - |
? | |
4-nitrophenyl myristate + H2O | - |
Pseudomonas aeruginosa | 4-nitrophenol + myristate | - |
? | |
4-nitrophenyl myristate + H2O | - |
Pseudomonas aeruginosa PseA | 4-nitrophenol + myristate | - |
? | |
4-nitrophenyl palmitate + H2O | - |
Pseudomonas aeruginosa | 4-nitrophenol + palmitate | - |
? | |
4-nitrophenyl palmitate + H2O | - |
Pseudomonas aeruginosa PseA | 4-nitrophenol + palmitate | - |
? | |
4-nitrophenyl stearate + H2O | - |
Pseudomonas aeruginosa | 4-nitrophenol + stearate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
lipase | - |
Pseudomonas aeruginosa |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
- |
Pseudomonas aeruginosa |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 50 | - |
Pseudomonas aeruginosa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Pseudomonas aeruginosa |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6 | 8.5 | - |
Pseudomonas aeruginosa |