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Literature summary for 3.1.1.3 extracted from

  • Gaur, R.; Gupta, A.; Khare, S.K.
    Purification and characterization of lipase from solvent tolerant Pseudomonas aeruginosa PseA (2008), Proc. Biochem., 43, 1040-1046.
No PubMed abstract available

Activating Compound

Activating Compound Comment Organism Structure
Fe3+ 132% relative activity after 30 min at 1 mM Pseudomonas aeruginosa
glutathione 112% relative activity after 30 min at 1 mM Pseudomonas aeruginosa

Inhibitors

Inhibitors Comment Organism Structure
2-mercaptoethanol reduces the enzyme activity to 48% after 1 h Pseudomonas aeruginosa
cetyltrimethylammonium bromide 45% residual activity after 5 h at 25% (v/v) Pseudomonas aeruginosa
CHAPS 86% residual activity after 5 h at 25% (v/v) Pseudomonas aeruginosa
Cu2+ 70% residual activity after 30 min at 8 mM Pseudomonas aeruginosa
diisopropylfluorophosphate 82% residual activity after 30 min at 8 mM Pseudomonas aeruginosa
hydrogen peroxide 62% residual activity after 5 h at 25% (v/v) Pseudomonas aeruginosa
additional information the enzyme activity is not affected significantly by Hg2+, Ca2+, Mg2+, Fe3+, EDTA and phenylmethylsulfonyl fluoride (1 mM each) Pseudomonas aeruginosa
p-chloromercuric benzoate 20% residual activity after 30 min at 8 mM Pseudomonas aeruginosa
sodium deoxycholate 90% residual activity after 5 h at 25% (v/v) Pseudomonas aeruginosa
sodium hypochlorite 85% residual activity after 5 h at 25% (v/v) Pseudomonas aeruginosa
Tween 20 74% residual activity after 5 h at 25% (v/v) Pseudomonas aeruginosa
Zn2+ 51% residual activity after 30 min at 1 mM Pseudomonas aeruginosa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
70.4
-
4-nitrophenyl palmitate in 0.1 M Tris-HCl buffer (pH 8.0), at 40°C Pseudomonas aeruginosa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
60000
-
SDS-PAGE Pseudomonas aeruginosa

Organic Solvent Stability

Organic Solvent Comment Organism
Brij 35 121% relative activity after 5 h at 25% (v/v) Pseudomonas aeruginosa
additional information the enzyme activity is not significantly affected by dimethylsulfonyloxide, isopropanol, ethanol, methanol, benzene, toluene, xylene, cyclohexane, hexane, n-heptane, isooctane, decane, and tetradecane Pseudomonas aeruginosa
Tween 80 124% relative activity after 5 h at 25% (v/v) Pseudomonas aeruginosa

Organism

Organism UniProt Comment Textmining
Pseudomonas aeruginosa
-
-
-
Pseudomonas aeruginosa PseA
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ultrafiltration and Sephadex G-100 gel filtration Pseudomonas aeruginosa

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
16.6
-
crude filtrate, in Tris-HCl, buffer (0.1 M, pH 8.0) Pseudomonas aeruginosa
143.3
-
after 8.6fold purification, in Tris-HCl, buffer (0.1 M, pH 8.0) Pseudomonas aeruginosa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3 triolein + 3 H2O
-
Pseudomonas aeruginosa 1,3-diolein + 1,2-diolein + 2,3-diolein + 3 oleate shows random positional specificity for triolein hydrolysis ?
4-nitrophenyl acetate + H2O
-
Pseudomonas aeruginosa 4-nitrophenol + acetate
-
?
4-nitrophenyl acetate + H2O
-
Pseudomonas aeruginosa PseA 4-nitrophenol + acetate
-
?
4-nitrophenyl butyrate + H2O
-
Pseudomonas aeruginosa 4-nitrophenol + butyrate
-
?
4-nitrophenyl butyrate + H2O
-
Pseudomonas aeruginosa PseA 4-nitrophenol + butyrate
-
?
4-nitrophenyl caprate + H2O
-
Pseudomonas aeruginosa 4-nitrophenol + caprate
-
?
4-nitrophenyl caprate + H2O
-
Pseudomonas aeruginosa PseA 4-nitrophenol + caprate
-
?
4-nitrophenyl myristate + H2O
-
Pseudomonas aeruginosa 4-nitrophenol + myristate
-
?
4-nitrophenyl myristate + H2O
-
Pseudomonas aeruginosa PseA 4-nitrophenol + myristate
-
?
4-nitrophenyl palmitate + H2O
-
Pseudomonas aeruginosa 4-nitrophenol + palmitate
-
?
4-nitrophenyl palmitate + H2O
-
Pseudomonas aeruginosa PseA 4-nitrophenol + palmitate
-
?
4-nitrophenyl stearate + H2O
-
Pseudomonas aeruginosa 4-nitrophenol + stearate
-
?

Synonyms

Synonyms Comment Organism
lipase
-
Pseudomonas aeruginosa

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
Pseudomonas aeruginosa

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
25 50
-
Pseudomonas aeruginosa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
Pseudomonas aeruginosa

pH Stability

pH Stability pH Stability Maximum Comment Organism
6 8.5
-
Pseudomonas aeruginosa