Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ag+ | isoyzme SCO1725 shows 11% residual activity in the presence of 1 mM Ag+; isozyme SCO7513 shows 10% residual activity in the presence of 1 mM Ag+ | Streptomyces coelicolor | |
Ca2+ | isoyzme SCO1725 shows 91% residual activity at 10 mM Ca2+; isozyme SCO7513 shows 86% residual activity in the presence of 1 mM Ca2+ | Streptomyces coelicolor | |
Co2+ | isoyzme SCO1725 shows 93% residual activity in the presence of 10 mM Co2+; isozyme SCO7513 shows 72% residual activity in the presence of 10 mM Co2+ | Streptomyces coelicolor | |
Cu2+ | isoyzme SCO1725 shows 88% residual activity at 1 mM Cu2+; isozyme SCO7513 shows 51% residual activity in the presence of 10 mM Cu2+ | Streptomyces coelicolor | |
dithiothreitol | isoyzme SCO1725 shows 36% residual activity in the presence of 10 mM dithiothreitol; isozyme SCO7513 shows 86% residual activity in the presence of 10 mM dithiothreitol | Streptomyces coelicolor | |
Mg2+ | isoyzme SCO1725 shows 92% residual activity in the presence of 10 mM Mg2+; isozyme SCO7513 shows 83% residual activity in the presence of 10 mM Mg2+ | Streptomyces coelicolor | |
additional information | Ca2+ and Mg2+ do not significantly reduce the lipolytic activity, resistant to inhibition by phenylmethylsulfonyl fluoride; Ca2+ and Mg2+ do not significantly reduce the lipolytic activity, resistant to inhibition by phenylmethylsulfonyl fluoride | Streptomyces coelicolor |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
23980 | - |
SCO1725, estimated molecular weight | Streptomyces coelicolor |
26970 | - |
SCO7513, estimated molecular weight | Streptomyces coelicolor |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces coelicolor | Q93J06 | - |
- |
Streptomyces coelicolor | Q9S2A5 | - |
- |
Streptomyces coelicolor A3(2) | Q93J06 | - |
- |
Streptomyces coelicolor A3(2) | Q9S2A5 | - |
- |
Purification (Comment) | Organism |
---|---|
Q Sepharose column chromatography, CM Sepharose FF column chromatography, and DEAE Sepharose column chromatography | Streptomyces coelicolor |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl caprate + H2O | - |
Streptomyces coelicolor | 4-nitrophenol + caprate | - |
? | |
4-nitrophenyl caprate + H2O | - |
Streptomyces coelicolor A3(2) | 4-nitrophenol + caprate | - |
? | |
4-nitrophenyl laurate + H2O | - |
Streptomyces coelicolor | 4-nitrophenol + laurate | - |
? | |
4-nitrophenyl laurate + H2O | - |
Streptomyces coelicolor A3(2) | 4-nitrophenol + laurate | - |
? | |
4-nitrophenyl palmitate + H2O | - |
Streptomyces coelicolor | 4-nitrophenol + palmitate | - |
? | |
4-nitrophenyl palmitate + H2O | - |
Streptomyces coelicolor A3(2) | 4-nitrophenol + palmitate | - |
? | |
4-nitrophenyl stearate + H2O | - |
Streptomyces coelicolor | 4-nitrophenol + stearate | - |
? | |
4-nitrophenyl stearate + H2O | - |
Streptomyces coelicolor A3(2) | 4-nitrophenol + stearate | - |
? | |
additional information | isozyme SCO1725 shows no activity on tricaprin, tricaproin, and olive oil, 4-nitrophenyl stearate is hardly hydrolysed, 4-nitrophenyl stearate is no substrate for isozyme SCO1725 | Streptomyces coelicolor | ? | - |
? | |
additional information | isozyme SCO1725 shows no activity on tricaprin, tricaproin, and olive oil, 4-nitrophenyl stearate is hardly hydrolysed, 4-nitrophenyl stearate is no substrate for isozyme SCO1725 | Streptomyces coelicolor A3(2) | ? | - |
? | |
tributyrin + H2O | partially hydrolysed by isozyme SCO1725 | Streptomyces coelicolor | dibutyrin + butyrate | - |
? | |
tributyrin + H2O | partially hydrolysed by isozyme SCO1725 | Streptomyces coelicolor A3(2) | dibutyrin + butyrate | - |
? | |
tricaprin + H2O | - |
Streptomyces coelicolor | dicaprin + decaprate | - |
? | |
tricaprylin + H2O | - |
Streptomyces coelicolor | dicaprylin + caprylate | - |
? | |
tricaprylin + H2O | - |
Streptomyces coelicolor A3(2) | dicaprylin + caprylate | - |
? | |
Tween 80 + H2O | - |
Streptomyces coelicolor | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
lipase | - |
Streptomyces coelicolor |
SCO1725 | isozyme | Streptomyces coelicolor |
SCO7513 | isozyme | Streptomyces coelicolor |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 30 | isozyme SCO1725 | Streptomyces coelicolor |
45 | 55 | isozyme SCO7513 | Streptomyces coelicolor |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 70 | isozyme SCO1725 is stable when incubated for 30 min at temperatures in the range of 30-70°C (at pH 7) | Streptomyces coelicolor |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7.5 | 9 | isozyme SCO1725 | Streptomyces coelicolor |
8.5 | 10 | isozyme SCO7513 | Streptomyces coelicolor |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
10 | 11 | the isozyme SCO1725 becomes unstable at pH over 10, where the activity drops to 34% at pH 10.5 and to 8% at pH 11 | Streptomyces coelicolor |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Streptomyces coelicolor | isozyme SCO7513, predicted value | - |
4.73 |
Streptomyces coelicolor | isozyme SCO1725, predicted value | - |
6.23 |