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Literature summary for 3.1.1.3 extracted from
- Cloning and fusion expression of a cold-active lipase from marine Antarctic origin (2008), Enzyme Microb. Technol., 42, 371-377.
No PubMed abstract available
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli strain TB1 | Psychrobacter sp. |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 47000 | - |
calculated from amino acid sequence | Psychrobacter sp. |
| 90000 | - |
SDS-PAGE, MBP-lipase as fusion protein | Psychrobacter sp. |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Psychrobacter sp. | Q9L514 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| amylose resin column chromatography | Psychrobacter sp. |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl acetate + H2O | - |
Psychrobacter sp. | 4-nitrophenol + acetate | - |
? |  |
| 4-nitrophenyl butyrate + H2O | - |
Psychrobacter sp. | 4-nitrophenol + butyrate | - |
? | |
| 4-nitrophenyl caproate + H2O | best substrate | Psychrobacter sp. | 4-nitrophenol + caproate | - |
? | |
| 4-nitrophenyl laurate + H2O | - |
Psychrobacter sp. | 4-nitrophenol + laurate | - |
? | |
| 4-nitrophenyl palmitate + H2O | - |
Psychrobacter sp. | 4-nitrophenol + palmitate | - |
? | |
| 4-nitrophenyl stearate + H2O | - |
Psychrobacter sp. | 4-nitrophenol + stearate | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| MBP-lipase | - |
Psychrobacter sp. |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | - |
- |
Psychrobacter sp. |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 5 | 40 | high specific activity even at 5°C, at temperatures higher 40°C the recombinant fusion protein is inactivated irreversibly, 50% active after 20 min of incubation at 30°C and pH 9.0, it is almost completely inactivated with incubation at 40 and 50°C for the same time | Psychrobacter sp. |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
- |
Psychrobacter sp. |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 11 | - |
Psychrobacter sp. |
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