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Literature summary for 3.1.1.3 extracted from

  • Wei, H.; Shi, L.; Wu, B.
    Production and characteristics of an enantioselective lipase from Burkholderia sp. GXU56 (2008), Chem. Eng. Technol., 31, 258-264.
No PubMed abstract available
Search for more literature for 3.1.1.3

Inhibitors

Inhibitors Comment Organism Structure
acetone 54% relative activity at 10% (v/v) acetone Burkholderia sp.
Co2+ 74% relative activity at 5 mM Burkholderia sp.
Cu2+ complete inhibition at 5 mM Burkholderia sp.
ethanol 56.2% relative activity at 10% (v/v) ethanol Burkholderia sp.
Fe2+ 36.9% relative activity at 5 mM Burkholderia sp.
Fe3+ 12.8% relative activity at 5 mM Burkholderia sp.
formaldehyde complete inhibition at 10% (v/v) Burkholderia sp.
Isopropanol 40.2% relative activity at 10% (v/v) isopropanol Burkholderia sp.
methanol 54.3% relative activity at 10% (v/v) methanol Burkholderia sp.
Mg2+ 96.6% relative activity at 5 mM Burkholderia sp.
Mn2+ 58.6% relative activity at 5 mM Burkholderia sp.
SDS 31.2% relative activity at 0.05% (w/v) SDS Burkholderia sp.
Zn2+ 28.9% relative activity at 5 mM Burkholderia sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.038
-
 4-nitrophenyl butyrate at pH 8.0 and 40°C Burkholderia sp.

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ 107.2% realtive activity at 5 mM Burkholderia sp.
Li+ 106.5% relative activity at 5 mM Burkholderia sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
35000
-
 SDS-PAGE Burkholderia sp.

Organic Solvent Stability

Organic Solvent Comment Organism
cetyltrimethylammonium bromidecetyltrimethylammonium bromide stimulated in the presence of 0.1% cetyltrimethylammonium bromidecetyltrimethylammonium bromide (145.3% relative activity) Burkholderia sp.
dimethylsulfoxide stimulated in the presence of 10% dimethylsulfoxide (105.1% relative activity) Burkholderia sp.
Triton X-100 stimulated in the presence of 0.1% Triton X-100 (110.2% relative activity) Burkholderia sp.

Organism

Organism UniProt Comment Textmining
Burkholderia sp.
-
-
-
Burkholderia sp. GXU56
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Mono Q column chromatography, octyl-Sepharose column chromatography, and Sepharyl S-200 gel filtration Burkholderia sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
61.42
-
 cell-free supernatant, at 40°C, pH 8.0 Burkholderia sp.
3294
-
 after 53.6fold purification, at 40°C, pH 8.0 Burkholderia sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl butyrate + H2O
-
 Burkholderia sp. 4-nitrophenol + butyrate
-
 ?
4-nitrophenyl butyrate + H2O
-
 Burkholderia sp. GXU56 4-nitrophenol + butyrate
-
 ?
methyl (R)-mandelate + H2O
-
 Burkholderia sp. (R)-mandelic acid + methanol
-
 ?
methyl (R)-mandelate + H2O
-
 Burkholderia sp. GXU56 (R)-mandelic acid + methanol
-
 ?

Synonyms

Synonyms Comment Organism
lipase
-
 Burkholderia sp.
triacylglycerol acylhydrolase
-
 Burkholderia sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
 Burkholderia sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
45
-
 stable below 45°C Burkholderia sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Burkholderia sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
7 8.5 retains 96.3% of the initial activity after preservation for 2 h at 30°C and pH 8.0 Burkholderia sp.