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Literature summary for 3.1.1.3 extracted from

  • Islam, M.A.; Absar, N.; Bhuiyan, A.S.
    Isolation, purification and characterization of lipase from gray mullet (Liza parsia Hamilton, 1822) (2008), Asian J. Biochem., 3, 243-255.
No PubMed abstract available
Search for more literature for 3.1.1.3

Inhibitors

Inhibitors Comment Organism Structure
Ca2+ isozyme Lip-1 shows 84% relative activity in the presence of 1 mM Ca2+; isozyme Lip-2 shows 75% relative activity in the presence of 1 mM Ca2+ Liza parsia
Cd2+ isozyme Lip-1 shows 38% relative activity in the presence of 5 mM Cd2+; isozyme Lip-2 shows 28% relative activity in the presence of 5 mM Cd2+ Liza parsia
Cu2+ isozyme Lip-1 shows 75% relative activity in the presence of 5 mM Cu2+; isozyme Lip-2 shows 65% relative activity in the presence of 5 mM Cu2+ Liza parsia
EDTA isozyme Lip-1 shows 25% relative activity in the presence of 5 mM EDTA; isozyme Lip-2 shows 35% relative activity in the presence of 5 mM EDTA Liza parsia
Hg2+ isozyme Lip-1 shows 20% relative activity in the presence of 5 mM Hg2+; isozyme Lip-2 shows 25% relative activity in the presence of 5 mM Hg2+ Liza parsia
Zn2+ isozyme Lip-1 shows 20% relative activity in the presence of 5 mM Zn2+; isozyme Lip-2 shows 18% relative activity in the presence of 5 mM Zn2+ Liza parsia

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ isozyme Lip-1 shows 114.4% relative activity in the presence of 5 mM Ca2+ Liza parsia
Ca2+ isozyme Lip-2 shows 105.2% relative activity in the presence of 5 mM Ca2+ Liza parsia

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
41200
-
 isozyme Lip-2, gel filtration Liza parsia
46500
-
 isozyme Lip-1, gel filtration Liza parsia

Organism

Organism UniProt Comment Textmining
Liza parsia
-
 gray mullet
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation, Sephadex G-50 gel filtration, CM-cellulsoe column chromatography, and DEAE-cellulose column chromatography Liza parsia

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
8.4
-
 crude extract Liza parsia
32.5
-
 after 37.75folf purification Liza parsia

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl laurate + H2O
-
 Liza parsia 4-nitrophenol + laurate
-
 ?
olive oil + H2O
-
 Liza parsia ?
-
 ?

Subunits

Subunits Comment Organism
dimer
-
 Liza parsia

Synonyms

Synonyms Comment Organism
Lip-1
-
 Liza parsia
Lip-2
-
 Liza parsia

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
33
-
 isozyme Lip-1 Liza parsia
35
-
 isozyme Lip-2 Liza parsia

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 60
-
 Liza parsia

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
33 35 activity is progressively lost beyond temperature optimum (33°C for isozyme Lip-1 and 35°C for isozyme Lip-2) Liza parsia

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 isozyme Lip-1 Liza parsia
8.5
-
 isozyme Lip-2 Liza parsia

pH Range

pH Minimum pH Maximum Comment Organism
7 8.5
-
 Liza parsia

pH Stability

pH Stability pH Stability Maximum Comment Organism
4 10 the isozymes Lip-1 and Lip-2 are stable between pH 7.0 and 10.0 at 25°C for 30 min, activity decreases gradually beyond pH 9.0 to pH 10, 55% residual activity above pH 7.0, marked loss of activity below pH 4.0 with 55% decrease in activity compared to the activity at pH 8.0 Liza parsia