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Literature summary for 3.1.1.3 extracted from

  • Yang, J.; Guo, D.; Yan, Y.
    Cloning, expression and characterization of a novel thermal stable and short-chain alcohol tolerant lipase from Burkholderia cepacia strain G63 (2007), J. Mol. Catal. B, 45, 91-96.
No PubMed abstract available
Search for more literature for 3.1.1.3

Cloned(Commentary)

Cloned (Comment) Organism
gene lipA, DNA and amino acid sequence determination and anaylsis, co-expression of lipA and lipB in Burkholderia cepacia strain G63 Burkholderia cepacia
gene lipB, DNA and amino acid sequence determination and anaylsis, co-expression of lipA and lipB in Burkholderia cepacia strain G63 Burkholderia cepacia

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
33000
-
 x * 33000, purified recombinant LipAB, SDS-PAGE Burkholderia cepacia

Organic Solvent Stability

Organic Solvent Comment Organism
amyl alcohol 48 h, 92.5% remaining activity, purified recombinant LipAB, incubation in 50% alcohol/water solution at room temperature Burkholderia cepacia
Ethanol 48 h, stable, purified recombinant LipAB, incubation in 50% alcohol/water solution at room temperature Burkholderia cepacia
Glycerol 48 h, stable, purified recombinant LipAB, incubation in 50% alcohol/water solution at room temperature Burkholderia cepacia
isopropanol 48 h, 91.7% remaining activity, purified recombinant LipAB, incubation in 50% alcohol/water solution at room temperature Burkholderia cepacia
Methanol 48 h, 98.34% remaining activity, purified recombinant LipAB, incubation in 50% alcohol/water solution at room temperature Burkholderia cepacia
n-propanol 48 h, 93.5% remaining activity, purified recombinant LipAB, incubation in 50% alcohol/water solution at room temperature Burkholderia cepacia

Organism

Organism UniProt Comment Textmining
Burkholderia cepacia A0EJ12 gene lipB; i.e. Pseudomonas cepacia, strain G63, gene lipB, isozyme LipB
-
Burkholderia cepacia Q4JL88 gene lipA; i.e. Pseudomonas cepacia, strain G63, gene lipA, isozyme LipA
-

Purification (Commentary)

Purification (Comment) Organism
recombinant LipAB 46.3fold from recombinant straon G63 Burkholderia cepacia

Reaction

Reaction Comment Organism Reaction ID
triacylglycerol + H2O = diacylglycerol + a carboxylate the catalytic center is formed by Ser87, Asp264 and His285 Burkholderia cepacia
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
olive oil + H2O
-
 Burkholderia cepacia ?
-
 ?

Subunits

Subunits Comment Organism
? x * 33000, purified recombinant LipAB, SDS-PAGE Burkholderia cepacia

Synonyms

Synonyms Comment Organism
LipA
-
 Burkholderia cepacia
LipB
-
 Burkholderia cepacia

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
70
-
-
 Burkholderia cepacia

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 85
-
 Burkholderia cepacia

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30 70 purified recombinant enzyme, stable within this range, 96.2% and 86.1% remaining activity after incubated at 70 °C for 2 h and 10 h, respectively Burkholderia cepacia
30 70 purified recombinant enzyme, stable within this range, 96.2% and 86.1% remaining activity after incubated at 70°C for 2 h and 10 h, respectively Burkholderia cepacia

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 assay at Burkholderia cepacia