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Literature summary for 3.1.1.3 extracted from
- Biochemical and molecular characterization of a lipase produced by Rhizopus oryzae (2006), FEMS Microbiol. Lett., 260, 241-248.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| additional information | ROL29 lipase shows the interfacial activation phenomenon when using tripropionin or vinyl propionate as substrates, while noninduced lipase ROLw does not | Rhizopus arrhizus |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| ROLw, DNA and amino acid sequence determination and analysis, expression in Escherichia coli strain DH5alpha | Rhizopus arrhizus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| tetrahydrolipstatin | inhibition of ROLw | Rhizopus arrhizus |  |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | secretion of noninduced lipase ROLw | Rhizopus arrhizus | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 29000 | - |
x * 29000, ROLw, SDS-PAGE | Rhizopus arrhizus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rhizopus arrhizus | Q2QFX1 | fragment; noninduced lipase ROLw | - |
| Rhizopus arrhizus WPG | Q2QFX1 | fragment; noninduced lipase ROLw | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native extracellular ROLw from fungal culture supernatant by gel filtration and cation exchange chromatography | Rhizopus arrhizus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| additional information | the maximal production of lipase is obtained at pH 6.0 and 30°C | Rhizopus arrhizus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 900 | - |
recombinant noninduced lipase ROLw, substrate tributyrin | Rhizopus arrhizus |
| 3500 | - |
recombinant noninduced lipase ROLw, substrate olive oil | Rhizopus arrhizus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | ROLw is unable to hydrolyse triacylglycerols in the presence of high concentration of bile salts, it is a serine enzyme | Rhizopus arrhizus | ? | - |
? | |
| additional information | ROLw is unable to hydrolyse triacylglycerols in the presence of high concentration of bile salts, it is a serine enzyme | Rhizopus arrhizus WPG | ? | - |
? | |
| olive oil + H2O | - |
Rhizopus arrhizus | ? | - |
? | |
| olive oil + H2O | - |
Rhizopus arrhizus WPG | ? | - |
? | |
| tributyrin + H2O | - |
Rhizopus arrhizus | dibutyrin + butyrate | - |
? | |
| tributyrin + H2O | - |
Rhizopus arrhizus WPG | dibutyrin + butyrate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 29000, ROLw, SDS-PAGE | Rhizopus arrhizus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| lipase | - |
Rhizopus arrhizus |
| noninduced lipase | - |
Rhizopus arrhizus |
| ROL29 | - |
Rhizopus arrhizus |
| ROLw | - |
Rhizopus arrhizus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
noninduced lipase ROLw | Rhizopus arrhizus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
noninduced lipase ROLw | Rhizopus arrhizus |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 8 | noninduced lipase ROLw, stable | Rhizopus arrhizus |
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