Any feedback?
Literature summary for 3.1.1.3 extracted from
- High yield expression of lipase A from Candida antarctica in the methylotrophic yeast Pichia pastoris and its purification and characterisation (2006), Appl. Microbiol. Biotechnol., 72, 931-938.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| CalA, subcloning in Escherichia coli strain DH5alpha, functional expression in Pichia pastoris strain X 33, development of a fed-batch process and a semi-continuous process, overview | Moesziomyces antarcticus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | kinetics | Moesziomyces antarcticus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| tributyrin + H2O | Moesziomyces antarcticus | - |
dibutyrin + butyrate | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Moesziomyces antarcticus | - |
strain DSM 70725, gene calA | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant CalA 3.1fold from Pichia pastoris, by cross-flow filtration and cation exchange chromatography | Moesziomyces antarcticus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 2033 | - |
purified recombinant enzyme | Moesziomyces antarcticus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | CalA shows preference for short-chain triglycerides, low activity with hydrophilic esters | Moesziomyces antarcticus | ? | - |
? | |
| tributyrin + H2O | - |
Moesziomyces antarcticus | dibutyrin + butyrate | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| CalA | - |
Moesziomyces antarcticus |
| lipase A | - |
Moesziomyces antarcticus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | 70 | - |
Moesziomyces antarcticus |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 10 | 70 | at temperatures over 70°C, the activity decreases dramatically, the activity increases continuously at temperatures from 10°C to 50°C, temperature profile, overview | Moesziomyces antarcticus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
- |
Moesziomyces antarcticus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 9 | rapid decrease in activity below pH 7.0 and above pH 9.0 | Moesziomyces antarcticus |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
