Cloned (Comment) | Organism |
---|---|
gene ALIPI, DNA and amino acid sequence determination and analysis, overexpression in strain G1211 and as His-tagged enzyme in Escherichia coli strain BL21(DE3) | Blastobotrys adeninivorans |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cd2+ | 50% inhibition | Blastobotrys adeninivorans | |
EDTA | 90% inhibition | Blastobotrys adeninivorans | |
Pyridine | 25-30% inhibition at about 30% v/v | Blastobotrys adeninivorans |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | the enzyme is secreted | Blastobotrys adeninivorans | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | about 30% activation | Blastobotrys adeninivorans | |
Fe3+ | about 30% activation | Blastobotrys adeninivorans | |
additional information | the enzyme is not or weakly affected by Mg2+, Mn2+, Cu2+, DTT, and 2-mercaptoethanol | Blastobotrys adeninivorans | |
Zn2+ | about 30% activation | Blastobotrys adeninivorans |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
45900 | - |
2 * 50000, native and recombinant enzyme, SDS-PAGE, 2 * 45900, native and recombinant deglycosylated enzyme, SDS-PAGE | Blastobotrys adeninivorans |
50000 | - |
2 * 50000, native and recombinant enzyme, SDS-PAGE, 2 * 45900, native and recombinant deglycosylated enzyme, SDS-PAGE | Blastobotrys adeninivorans |
100000 | - |
native and recombinant enzyme, gel filtration | Blastobotrys adeninivorans |
Organic Solvent | Comment | Organism |
---|---|---|
additional information | the purified enzyme is stable to organic solvents with the exception of pyridine, which inhibits 25-30% of the activity, overview | Blastobotrys adeninivorans |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Blastobotrys adeninivorans | Q5GMI6 | dimorphic yeast, gene ALIPI | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | analysis of carbohydrate content | Blastobotrys adeninivorans |
Purification (Comment) | Organism |
---|---|
native enzyme 7.4fold by ion exchange chromatography and gel filtration | Blastobotrys adeninivorans |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
367 | - |
purified native enzyme | Blastobotrys adeninivorans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl caprate + H2O | - |
Blastobotrys adeninivorans | 4-nitrophenol + caprate | - |
? | |
4-nitrophenyl caprylate + H2O | - |
Blastobotrys adeninivorans | 4-nitrophenol + caprylate | - |
? | |
additional information | the enzyme prefers substrates containing middle-size fatty acids | Blastobotrys adeninivorans | ? | - |
? | |
tricaprylin + H2O | best substrate | Blastobotrys adeninivorans | dicaprylin + caprylate | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 50000, native and recombinant enzyme, SDS-PAGE, 2 * 45900, native and recombinant deglycosylated enzyme, SDS-PAGE | Blastobotrys adeninivorans |
Synonyms | Comment | Organism |
---|---|---|
Alip1p | - |
Blastobotrys adeninivorans |
extracellular lipase | - |
Blastobotrys adeninivorans |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
native and recombinant enzyme | Blastobotrys adeninivorans |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
native and recombinant enzyme, stable up to | Blastobotrys adeninivorans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
native and recombinant enzyme | Blastobotrys adeninivorans |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
7 | 7.5 | native and recombinant enzyme, stable | Blastobotrys adeninivorans |