Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.1.1.3 extracted from

  • Böer, E.; Mock, H.P.; Bode, R.; Gellissen, G.; Kunze, G.
    An extracellular lipase from the dimorphic yeast Arxula adeninivorans: molecular cloning of the ALIP1 gene and characterization of the purified recombinant enzyme (2005), Yeast, 22, 523-535.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
gene ALIPI, DNA and amino acid sequence determination and analysis, overexpression in strain G1211 and as His-tagged enzyme in Escherichia coli strain BL21(DE3) Blastobotrys adeninivorans

Inhibitors

Inhibitors Comment Organism Structure
Cd2+ 50% inhibition Blastobotrys adeninivorans
EDTA 90% inhibition Blastobotrys adeninivorans
Pyridine 25-30% inhibition at about 30% v/v Blastobotrys adeninivorans

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular the enzyme is secreted Blastobotrys adeninivorans
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ about 30% activation Blastobotrys adeninivorans
Fe3+ about 30% activation Blastobotrys adeninivorans
additional information the enzyme is not or weakly affected by Mg2+, Mn2+, Cu2+, DTT, and 2-mercaptoethanol Blastobotrys adeninivorans
Zn2+ about 30% activation Blastobotrys adeninivorans

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
45900
-
2 * 50000, native and recombinant enzyme, SDS-PAGE, 2 * 45900, native and recombinant deglycosylated enzyme, SDS-PAGE Blastobotrys adeninivorans
50000
-
2 * 50000, native and recombinant enzyme, SDS-PAGE, 2 * 45900, native and recombinant deglycosylated enzyme, SDS-PAGE Blastobotrys adeninivorans
100000
-
native and recombinant enzyme, gel filtration Blastobotrys adeninivorans

Organic Solvent Stability

Organic Solvent Comment Organism
additional information the purified enzyme is stable to organic solvents with the exception of pyridine, which inhibits 25-30% of the activity, overview Blastobotrys adeninivorans

Organism

Organism UniProt Comment Textmining
Blastobotrys adeninivorans Q5GMI6 dimorphic yeast, gene ALIPI
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein analysis of carbohydrate content Blastobotrys adeninivorans

Purification (Commentary)

Purification (Comment) Organism
native enzyme 7.4fold by ion exchange chromatography and gel filtration Blastobotrys adeninivorans

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
367
-
purified native enzyme Blastobotrys adeninivorans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl caprate + H2O
-
Blastobotrys adeninivorans 4-nitrophenol + caprate
-
?
4-nitrophenyl caprylate + H2O
-
Blastobotrys adeninivorans 4-nitrophenol + caprylate
-
?
additional information the enzyme prefers substrates containing middle-size fatty acids Blastobotrys adeninivorans ?
-
?
tricaprylin + H2O best substrate Blastobotrys adeninivorans dicaprylin + caprylate
-
?

Subunits

Subunits Comment Organism
dimer 2 * 50000, native and recombinant enzyme, SDS-PAGE, 2 * 45900, native and recombinant deglycosylated enzyme, SDS-PAGE Blastobotrys adeninivorans

Synonyms

Synonyms Comment Organism
Alip1p
-
Blastobotrys adeninivorans
extracellular lipase
-
Blastobotrys adeninivorans

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
native and recombinant enzyme Blastobotrys adeninivorans

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
native and recombinant enzyme, stable up to Blastobotrys adeninivorans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
native and recombinant enzyme Blastobotrys adeninivorans

pH Stability

pH Stability pH Stability Maximum Comment Organism
7 7.5 native and recombinant enzyme, stable Blastobotrys adeninivorans