Cloned (Comment) | Organism |
---|---|
DNA and amino acid sequence determination and analysis, expression of GST-tagged enzyme in Escherichia coli | Geobacillus stearothermophilus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | recombinant enzyme | Geobacillus stearothermophilus | |
Li+ | recombinant enzyme | Geobacillus stearothermophilus | |
Mn2+ | recombinant enzyme | Geobacillus stearothermophilus | |
additional information | no inhibition by EDTA, PMSF, 2-mercaptoethanol, PMSF, DTT, Tween 20, 3-[(3-cholamidopropyl)dimethylammonio]-1-propane sulfonic acid, or Triton X-100 | Geobacillus stearothermophilus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | activates, recombinant enzyme | Geobacillus stearothermophilus | |
Fe2+ | activates, recombinant enzyme | Geobacillus stearothermophilus | |
Fe3+ | activates, recombinant enzyme | Geobacillus stearothermophilus | |
Mg2+ | activates, recombinant enzyme | Geobacillus stearothermophilus | |
Zn2+ | activates, recombinant enzyme | Geobacillus stearothermophilus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Geobacillus stearothermophilus | Q3T5M2 | i.e. Bacillus stearothermophilus, strain TW1, isolated from a hot spring in China, gene lip2 | - |
Purification (Comment) | Organism |
---|---|
recombinant GST-tagged enzyme from Escherichia coli | Geobacillus stearothermophilus |
Synonyms | Comment | Organism |
---|---|---|
LIP2 | - |
Geobacillus stearothermophilus |
lipase | - |
Geobacillus stearothermophilus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
optimal growth temperature is 65°C | Geobacillus stearothermophilus |
40 | - |
recombinant enzyme | Geobacillus stearothermophilus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
90 | - |
stable up to, purified recombinant enzyme | Geobacillus stearothermophilus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | 8 | recombinant enzyme | Geobacillus stearothermophilus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5 | 11 | stable, purified recombinant enzyme | Geobacillus stearothermophilus |