Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Dichloromethane | 55% activation of recombinant refolded enzyme at 30% v/v, 67% at 10% v/v | Ralstonia sp. | |
dimethylformamide | 28% activation of recombinant refolded enzyme at 10% v/v, 57% inhibition of recombinant refolded enzyme at 30% v/v | Ralstonia sp. | |
DMSO | 64% activation of recombinant refolded enzyme at 10% v/v, no effect at 30% v/v | Ralstonia sp. | |
ethanol | 43% activation of recombinant refolded enzyme at 10% v/v, and 44% inhibition at 30% v/v | Ralstonia sp. | |
ethanolamine | 234% activation of recombinant refolded enzyme at 30% v/v, 37% at 10% v/v | Ralstonia sp. | |
ethyl acetate | 98% activation of recombinant refolded enzyme at 30% v/v | Ralstonia sp. | |
hexane | 53% activation of recombinant refolded enzyme at 10-30% v/v | Ralstonia sp. | |
Isopropanol | 39% activation of recombinant refolded enzyme at 10% v/v, and 44% inhibition at 30% v/v | Ralstonia sp. | |
additional information | organic solvents induce the enzyme | Ralstonia sp. | |
SDS | 83% inhibition of the recombinant refolded enzyme at 5% w/v, 52% activation of the recombinant refolded enzyme at 1% w/v | Ralstonia sp. | |
tert-butanol | 41% activation of recombinant refolded enzyme at 30% v/v | Ralstonia sp. | |
Triton X-100 | 19-39% activation of the recombinant refolded enzyme at 0.2-1% w/v, 89% inhibition of the recombinant refolded enzyme at 5% w/v | Ralstonia sp. | |
Triton X-45 | 42-45% activation of the recombinant refolded enzyme at 0.2-1% w/v, 31% inhibition of the recombinant refolded enzyme at 5% w/v | Ralstonia sp. | |
Tween 40 | recombinant refolded enzyme | Ralstonia sp. | |
Tween 60 | recombinant refolded enzyme | Ralstonia sp. | |
Tween 80 | recombinant refolded enzyme | Ralstonia sp. |
Cloned (Comment) | Organism |
---|---|
overexpression of LipA and a 56-amino acid truncated chaperone DELTALipBHis6 in Escherichia coli strain Bl21, LIpA in expressed in inclusion bodies | Ralstonia sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1-butanol | 47% inhibition of recombinant refolded enzyme at 10% v/v | Ralstonia sp. | |
2-mercaptoethanol | maximal inhibition of 32% at 1 mM of recombinant refolded enzyme | Ralstonia sp. | |
acetone | 45% inhibition of recombinant refolded enzyme at 30% v/v | Ralstonia sp. | |
acetonitrile | 60% inhibition of recombinant refolded enzyme at 30% v/v | Ralstonia sp. | |
Ca2+ | 14% activation of recombinant refolded enzyme at 0.1 mM, 17% inhibition at 10 mM | Ralstonia sp. | |
diethyldicarbonate | 15% inhibition of recombinant refolded enzyme at 10 mM | Ralstonia sp. | |
dimethylformamide | 57% inhibition of recombinant refolded enzyme at 30% v/v, 28% activation of recombinant refolded enzyme at 10% v/v | Ralstonia sp. | |
EDTA | 27-29% inhibition of recombinant refolded enzyme at 0.1-10 mM | Ralstonia sp. | |
ethanol | 43% activation of recombinant refolded enzyme at 10% v/v, and 44% inhibition at 30% v/v | Ralstonia sp. | |
Fe3+ | 14% inhibition of recombinant refolded enzyme at 10 mM, 23% at 1 mM, 18% at 0.1 mM | Ralstonia sp. | |
Isopropanol | 39% activation of recombinant refolded enzyme at 10% v/v, and 44% inhibition at 30% v/v | Ralstonia sp. | |
Mg2+ | 10% activation of recombinant refolded enzyme at 1 mM, 15% inhibition at 10 mM | Ralstonia sp. | |
PMSF | 43% inhibition of recombinant refolded enzyme at 10 mM, 15% at 1 mM | Ralstonia sp. | |
SDS | 52% activation of the recombinant refolded enzyme at 1% w/v, 83% inhibition of the recombinant refolded enzyme at 5% w/v | Ralstonia sp. | |
Triton X-100 | 19-39% activation of the recombinant refolded enzyme at 0.2-1% w/v, 89% inhibition of the recombinant refolded enzyme at 5% w/v | Ralstonia sp. | |
Triton X-45 | 42-45% activation of the recombinant refolded enzyme at 0.2-1% w/v, 31% inhibition of the recombinant refolded enzyme at 5% w/v | Ralstonia sp. | |
Zn2+ | 70% inhibition of recombinant refolded enzyme at 10 mM, 66% at 1 mM, 35% at 0.1 mM | Ralstonia sp. |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 14% activation of recombinant refolded enzyme at 0.1 mM, 17% inhibition at 10 mM | Ralstonia sp. | |
Cu2+ | no activation of recombinant refolded enzyme at 10 mM and 1 mM, 14% at 0.1 mM | Ralstonia sp. | |
Mg2+ | 10% activation of recombinant refolded enzyme at 1 mM, 15% inhibition at 10 mM | Ralstonia sp. | |
Mn2+ | 22% activation of recombinant refolded enzyme at 10 mM | Ralstonia sp. | |
Sn2+ | no activation of recombinant refolded enzyme at 10 mM and 1 mM, 12% at 0.1 mM | Ralstonia sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
32500 | - |
x * 32500, recombinant enzyme | Ralstonia sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
triacylglycerol + H2O | Ralstonia sp. | of several natural oils, e.g. cottonseed oil, corn oil, palm oil, wheatgerm oil, soybean oil, rapeseed oil, coconut oil, linseed oil, peanut oil, and olive oil in descending activity, overview | diacylglycerol + a carboxylate | - |
? | |
triacylglycerol + H2O | Ralstonia sp. M1 | of several natural oils, e.g. cottonseed oil, corn oil, palm oil, wheatgerm oil, soybean oil, rapeseed oil, coconut oil, linseed oil, peanut oil, and olive oil in descending activity, overview | diacylglycerol + a carboxylate | - |
? | |
tributyrin + H2O | Ralstonia sp. | - |
dibutyrin + butyrate | - |
? | |
tributyrin + H2O | Ralstonia sp. M1 | - |
dibutyrin + butyrate | - |
? | |
tricaprylin + H2O | Ralstonia sp. | - |
dicaprylin + caprylate | - |
? | |
triolein + H2O | Ralstonia sp. | olive oil | diolein + oleate | - |
? | |
triolein + H2O | Ralstonia sp. M1 | olive oil | diolein + oleate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Ralstonia sp. | - |
gene lipA | - |
Ralstonia sp. M1 | - |
gene lipA | - |
Purification (Comment) | Organism |
---|---|
recombinant wild-type LipA and a 56-amino acid truncated chaperone DELTALipBHis6 from Escherichia coli strain BL21 by nickel affinity chromatography | Ralstonia sp. |
Renatured (Comment) | Organism |
---|---|
refolding of wild-type LipA from inclusion bodies solublized by 8 M urea, refolded with a 56-amino acid truncated chaperone DELTALipBHis6 at 4°C for 24 h in water in a ratio of 1:1 | Ralstonia sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl caprylate + H2O | preferred 4-nitrophenyl substrate of the recombinant enzyme | Ralstonia sp. | 4-nitrophenol + caprylate | - |
? | |
4-nitrophenyl caprylate + H2O | preferred 4-nitrophenyl substrate of the recombinant enzyme | Ralstonia sp. M1 | 4-nitrophenol + caprylate | - |
? | |
4-nitrophenyl myristate + H2O | preferred 4-nitrophenyl substrate of the wild-type enzyme | Ralstonia sp. | 4-nitrophenol + myristate | - |
? | |
additional information | substrate specificity of the recombinant and the wild-type enzyme, preference for triacylglycerides with short length fatty acids | Ralstonia sp. | ? | - |
? | |
additional information | substrate specificity of the recombinant and the wild-type enzyme, preference for triacylglycerides with short length fatty acids | Ralstonia sp. M1 | ? | - |
? | |
triacylglycerol + H2O | of several natural oils, e.g. cottonseed oil, corn oil, palm oil, wheatgerm oil, soybean oil, rapeseed oil, coconut oil, linseed oil, peanut oil, and olive oil in descending activity, overview | Ralstonia sp. | diacylglycerol + a carboxylate | - |
? | |
triacylglycerol + H2O | of several natural oils, e.g. cottonseed oil, corn oil, palm oil, wheatgerm oil, soybean oil, rapeseed oil, coconut oil, linseed oil, peanut oil, and olive oil in descending activity, overview | Ralstonia sp. M1 | diacylglycerol + a carboxylate | - |
? | |
tributyrin + H2O | - |
Ralstonia sp. | dibutyrin + butyrate | - |
? | |
tributyrin + H2O | - |
Ralstonia sp. M1 | dibutyrin + butyrate | - |
? | |
tricaprylin + H2O | - |
Ralstonia sp. | dicaprylin + caprylate | - |
? | |
triolein + H2O | olive oil | Ralstonia sp. | diolein + oleate | - |
? | |
triolein + H2O | olive oil | Ralstonia sp. M1 | diolein + oleate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 32500, recombinant enzyme | Ralstonia sp. |
Synonyms | Comment | Organism |
---|---|---|
LipA | - |
Ralstonia sp. |
lipase | - |
Ralstonia sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | 55 | recombinant refolded enzyme | Ralstonia sp. |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 75 | about 20% of maximal activity at 25°C, 60% at 75°C, recombinant refolded enzyme | Ralstonia sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
stable up to, recombinant refolded enzyme | Ralstonia sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
10.8 | - |
recombinant refolded enzyme, narrow optimum | Ralstonia sp. |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
8.5 | 11.5 | about 20% of maximal activity at pH 10.0 and pH 11.0, recombinant refolded enzyme | Ralstonia sp. |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
8 | 10.5 | 52% remaining activity, recombinant refolded enzyme | Ralstonia sp. |