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Literature summary for 3.1.1.3 extracted from
- The Pseudomonas fluorescens lipase has a C-terminal secretion signal and is secreted by a three-component bacterial ABC-exporter system (1994), Mol. Microbiol., 11, 1117-1126.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| additional information | tributyrin induces the extracellular enzyme | Pseudomonas fluorescens |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| functional expression of extracellular enzyme in Pseudomonas aeruginosa strains PAO25 and PAO25 ME3, secretion of recombinant enzyme | Pseudomonas fluorescens |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | lipase has a C-terminal secretion signal and is secreted by a three-component bacterial ABC-exporter system, mechanism | Pseudomonas fluorescens | - |
- |
| extracellular | the extracellular enzyme contains an N-terminal secretion signal sequence, mechanism | Pseudomonas aeruginosa | - |
- |
| intracellular | - |
Pseudomonas fluorescens | 5622 | - |
| intracellular | - |
Pseudomonas aeruginosa | 5622 | - |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas aeruginosa | - |
strains PAO and PAO25 ME3 | - |
| Pseudomonas fluorescens | - |
gene LIP | - |
| Pseudomonas fluorescens B52 | - |
gene LIP | - |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| culture condition:tributyrin-grown cell | - |
Pseudomonas fluorescens | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
activities of the extra- and the intracellular enzyme forms in strain B52 | Pseudomonas fluorescens |
| additional information | - |
activities of the extra- and the intracellular enzyme forms in strains PAO25 and PAO25 ME3 | Pseudomonas aeruginosa |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| tributyrin + H2O | - |
Pseudomonas fluorescens | dibutyrin + butyrate | - |
? |  |
| tributyrin + H2O | - |
Pseudomonas aeruginosa | dibutyrin + butyrate | - |
? | |
| tributyrin + H2O | - |
Pseudomonas fluorescens B52 | dibutyrin + butyrate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| More | extracellular lipase contains a C-terminal secretion signal, but bo N-terminal signal sequence | Pseudomonas fluorescens |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| lipase | - |
Pseudomonas fluorescens |
| lipase | - |
Pseudomonas aeruginosa |
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