Inhibitors | Comment | Organism | Structure |
---|---|---|---|
butanol | 70%, 30°C, complete immediate inactivation | Geobacillus thermoleovorans | |
Hg+ | - |
Geobacillus thermoleovorans | |
K+ | - |
Geobacillus thermoleovorans | |
Li+ | - |
Geobacillus thermoleovorans | |
Mg2+ | - |
Geobacillus thermoleovorans |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | activates | Geobacillus thermoleovorans | |
additional information | no effect by Ba2+ and Na+ | Geobacillus thermoleovorans |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
triolein + H2O | Geobacillus thermoleovorans | olive oil | diolein + oleate | - |
? | |
triolein + H2O | Geobacillus thermoleovorans CCR11 | olive oil | diolein + oleate | - |
? |
Organic Solvent | Comment | Organism |
---|---|---|
2-propanol | 70%, 30°C, completely stable for 1 h, loss of 18% activity after 2h | Geobacillus thermoleovorans |
Acetone | 70%, 30°C, completely stable for 1 h, loss of 60% activity after 2h | Geobacillus thermoleovorans |
Butanol | 70%, 30°C, complete immediate inactivation | Geobacillus thermoleovorans |
Ethanol | 70%, 30°C, completely stable for 1 h, loss of 30% activity after 2h | Geobacillus thermoleovorans |
Methanol | 70%, 30°C, loss of 35% activity after 2h | Geobacillus thermoleovorans |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Geobacillus thermoleovorans | - |
isolated from a hot spring in Mexico | - |
Geobacillus thermoleovorans CCR11 | - |
isolated from a hot spring in Mexico | - |
Purification (Comment) | Organism |
---|---|
native enzyme from cell-free supernatant by isoelectric focusing, PAGE, and ultrafiltration | Geobacillus thermoleovorans |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | optimal growth at pH 6.5, 55°C in presence of 1% gum arabic, 2.5% olive oil and 0.1% w/v CaCl2 | Geobacillus thermoleovorans | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl laurate + H2O | - |
Geobacillus thermoleovorans | 4-nitrophenol + laurate | - |
? | |
4-nitrophenyl laurate + H2O | - |
Geobacillus thermoleovorans CCR11 | 4-nitrophenol + laurate | - |
? | |
triolein + H2O | olive oil | Geobacillus thermoleovorans | diolein + oleate | - |
? | |
triolein + H2O | olive oil | Geobacillus thermoleovorans CCR11 | diolein + oleate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
lipase | - |
Geobacillus thermoleovorans |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
- |
Geobacillus thermoleovorans |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
pH 6.5, 1 h, completely stable, 26 h, over 80% remaining activity | Geobacillus thermoleovorans |
50 | 60 | pH 6.5, 1 h, 75% remaining activity | Geobacillus thermoleovorans |
70 | - |
pH 6.5, 1 h, nearly complete loss of activity | Geobacillus thermoleovorans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | 10 | - |
Geobacillus thermoleovorans |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5 | 11 | purified enzyme, stable | Geobacillus thermoleovorans |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Geobacillus thermoleovorans | isoelectric focusing | - |
6 |