Literature summary for 3.1.1.3 extracted from

Jaeger, K.E.; Adrian, F.J.; Meyer, H.E.; Hancock, R.E.W.; Winkler, U.K.
Extracellular lipase from Pseudomonas aeruginosa is an amphiphilic protein (1992), Biochim. Biophys. Acta, 1120, 315-321.

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Inhibitors

Inhibitors	Comment	Organism	Structure
diethyl 4-nitrophenyl phosphate	rapid and complete inhibition	Pseudomonas aeruginosa

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
extracellular	the enzyme is secreted	Pseudomonas aeruginosa	-	-

Organism

Organism	UniProt	Comment	Textmining
Pseudomonas aeruginosa	-	-	-
Pseudomonas aeruginosa PAC1R	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
extracellular enzyme by chromatographical isoelectric focusing	Pseudomonas aeruginosa

Reaction

Reaction	Comment	Organism	Reaction ID
triacylglycerol + H2O = diacylglycerol + a carboxylate	the enzyme contains an active site serine residue	Pseudomonas aeruginosa	a

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
4-nitrophenyl palmitate + H2O	-	Pseudomonas aeruginosa	4-nitrophenol + palmitate	-	?
4-nitrophenyl palmitate + H2O	-	Pseudomonas aeruginosa PAC1R	4-nitrophenol + palmitate	-	?

Subunits

Subunits	Comment	Organism
More	the enzyme contains the conserved N-terminal consensus sequence KYP-IVL-VHG, sodium deoxycholate and cetyltrimethylammonium bromide shift the electrophoretic mobility of the enzyme in anodal or cathodal direction, respectively, amphiphilic enzyme	Pseudomonas aeruginosa

Synonyms

Synonyms	Comment	Organism
extracellular lipase	-	Pseudomonas aeruginosa
lipase	-	Pseudomonas aeruginosa
triacylglycerol acylhydrolase	-	Pseudomonas aeruginosa

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
20	-	assay at	Pseudomonas aeruginosa

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
6	-	assay at	Pseudomonas aeruginosa

pI Value

Organism	Comment	pI Value Maximum	pI Value
Pseudomonas aeruginosa	isoelectric focusing	-	5.7

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Release 2023.1 (January 2023)