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Literature summary for 3.1.1.3 extracted from
- High-level expression of a lipase from Bacillus thermocatenulatus BTL2 in Pichia pastoris and some properties of the recombinant lipase (2003), Protein Expr. Purif., 28, 102-110.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| amino acid determinatioin of the purified enzyme, high-level expression in Pichia pastoris GS115, subcloning in Escherichia coli DH5alpha | Geobacillus thermocatenulatus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2-propanol | - |
Geobacillus thermocatenulatus |  |
| acetone | - |
Geobacillus thermocatenulatus | |
| cholate | 1% w/v, 20% decrease in activity | Geobacillus thermocatenulatus | |
| methanol | - |
Geobacillus thermocatenulatus | |
| SDS | 1% w/v, 95% decrease in activity | Geobacillus thermocatenulatus | |
| Triton X-100 | 1% w/v, 30% decrease in activity | Geobacillus thermocatenulatus | |
| Tween 20 | 1% w/v, 7% decrease in activity | Geobacillus thermocatenulatus | |
| Tween 80 | 1% w/v, 6% decrease in activity | Geobacillus thermocatenulatus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 43000 | - |
x * 43000, recombinant enzyme, SDS-PAGE | Geobacillus thermocatenulatus |
Organic Solvent Stability
| Organic Solvent | Comment | Organism |
|---|---|---|
| 2-propanol | 30% v/v, 1 h, 30°C, 0.1 M Tris buffer, pH 8.0, 47% reduced activity | Geobacillus thermocatenulatus |
| Acetone | 30% v/v, 1 h, 30°C, 0.1 M Tris buffer, pH 8.0, 16% reduced activity | Geobacillus thermocatenulatus |
| Methanol | 30% v/v, 1 h, 30°C, 0.1 M Tris buffer, pH 8.0, 16% reduced activity | Geobacillus thermocatenulatus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Geobacillus thermocatenulatus | - |
- |
- |
| Geobacillus thermocatenulatus BTL2 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| large scale from Pichia pastoris culture supernatant, 660fold | Geobacillus thermocatenulatus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 23000 | - |
purified enzyme, substrate tributyrin | Geobacillus thermocatenulatus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl palmitate + H2O | - |
Geobacillus thermocatenulatus | 4-nitrophenol + palmitate | - |
? | |
| 4-nitrophenyl palmitate + H2O | - |
Geobacillus thermocatenulatus BTL2 | 4-nitrophenol + palmitate | - |
? | |
| triacetin + H2O | poor substrate | Geobacillus thermocatenulatus | diacetin + acetate | - |
? | |
| tributyrin + H2O | best substrate | Geobacillus thermocatenulatus | dibutyrin + butyrate | - |
? | |
| tributyrin + H2O | best substrate | Geobacillus thermocatenulatus BTL2 | dibutyrin + butyrate | - |
? | |
| tricaprin + H2O | about 20% of the activity with tributyrin | Geobacillus thermocatenulatus | dicaprin + decaprate | - |
? | |
| tricaproin + H2O | about 20% of the activity with tributyrin | Geobacillus thermocatenulatus | dicaproin + caproate | - |
? | |
| trilaurin + H2O | about 10% of the activity with tributyrin | Geobacillus thermocatenulatus | dilaurin + laurate | - |
? | |
| trilaurin + H2O | about 10% of the activity with tributyrin | Geobacillus thermocatenulatus BTL2 | dilaurin + laurate | - |
? | |
| trimyristin + H2O | about 10% of the activity with tributyrin | Geobacillus thermocatenulatus | dimyristin + myristate | - |
? | |
| triolein + H2O | - |
Geobacillus thermocatenulatus | diolein + oleate | - |
? | |
| triolein + H2O | - |
Geobacillus thermocatenulatus BTL2 | diolein + oleate | - |
? | |
| tripalmitin + H2O | about 20% of the activity with tributyrin | Geobacillus thermocatenulatus | dipalmitin + palmitate | - |
? | |
| tripalmitin + H2O | about 20% of the activity with tributyrin | Geobacillus thermocatenulatus BTL2 | dipalmitin + palmitate | - |
? | |
| tristearin + H2O | about 10% of the activity with tributyrin | Geobacillus thermocatenulatus | distearin + stearate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 43000, recombinant enzyme, SDS-PAGE | Geobacillus thermocatenulatus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| lipase | - |
Geobacillus thermocatenulatus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 65 | - |
with substrate tributyrin, pH 8.0 | Geobacillus thermocatenulatus |
| 75 | - |
with substrate triolein, pH 8.0 | Geobacillus thermocatenulatus |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | 80 | - |
Geobacillus thermocatenulatus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
stable up to, pH 8.0, 0.1 M Tris buffer | Geobacillus thermocatenulatus |
| 70 | - |
18% remaining activity | Geobacillus thermocatenulatus |
| 80 | - |
5% remaining activity | Geobacillus thermocatenulatus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
with substrate tributyrin, 60°C | Geobacillus thermocatenulatus |
| 9 | - |
with substrate triolein, 60°C | Geobacillus thermocatenulatus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 9 | - |
Geobacillus thermocatenulatus |
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