Cloned (Comment) | Organism |
---|---|
DNA and amino acid sequence determination and analysis, expression in Escherichia coli M15 with a N-terminal His-tagged substituting the signal sequence, the expressed protein is insoluble and inactive, expression of the C-terminally His-tagged enzyme containing its native signal sequence in Spodoptera frugiperda Sf9 insect cells via baculovirus infection results in active enzyme | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl laurate + H2O | Homo sapiens | recombinant enzyme in Sf9 cells | 4-nitrophenol + laurate | - |
? | |
additional information | Homo sapiens | the enzyme mobilizes stored triacylglycerols some of which is used for very-low-density lipoprotein assembly in the liver | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | the recombinant enzyme from Sf9 insect cells becomes glycosylated in the endoplasmic reticulum | Homo sapiens |
Purification (Comment) | Organism |
---|---|
recombinant from Sf9 insect cells, about 30fold | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl laurate + H2O | - |
Homo sapiens | 4-nitrophenol + laurate | - |
? | |
4-nitrophenyl laurate + H2O | recombinant enzyme in Sf9 cells | Homo sapiens | 4-nitrophenol + laurate | - |
? | |
additional information | the enzyme mobilizes stored triacylglycerols some of which is used for very-low-density lipoprotein assembly in the liver | Homo sapiens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
More | enzyme belongs to the carboxylesterase family | Homo sapiens |
TGH | - |
Homo sapiens |
triacylglycerol hydrolase | - |
Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Homo sapiens |