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Literature summary for 3.1.1.3 extracted from
- Optimization of a thermostable lipase from Bacillus stearothermophilus P1: overexpression, purification, and characterization (2001), Protein Expr. Purif., 22, 388-398.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| construction of a genetic library from genomic DNA, P1, DNA and amino acid sequence determination and analysis, expression in Escherichia coli M15 | Geobacillus stearothermophilus |
General Stability
| General Stability | Organism |
|---|---|
| stable for 1 h at 37°C in 0.1% CHAPS and Triton X-100 | Geobacillus stearothermophilus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 1-hexandecanesulfonyl chloride | complete inhibition at 10 mM | Geobacillus stearothermophilus |  |
| 2-mercaptoethanol | slight inhibition | Geobacillus stearothermophilus | |
| CHAPS | inhibits at 1% | Geobacillus stearothermophilus | |
| Cs+ | slight inhibition | Geobacillus stearothermophilus | |
| dithiothreitol | - |
Geobacillus stearothermophilus | |
| dodecanesulfonyl chloride | - |
Geobacillus stearothermophilus | |
| EDTA | - |
Geobacillus stearothermophilus | |
| Fe2+ | 47% inhibition at 1 mM, 99.2% inhibition at 10 mM | Geobacillus stearothermophilus | |
| K+ | slight inhibition | Geobacillus stearothermophilus | |
| Li+ | slight inhibition | Geobacillus stearothermophilus | |
| Mn2+ | slight inhibition | Geobacillus stearothermophilus | |
| PMSF | strong inhibition at 10 mM | Geobacillus stearothermophilus | |
| SDS | inhibits at 0.1% | Geobacillus stearothermophilus | |
| sodium deoxycholate | inhibits at 1% | Geobacillus stearothermophilus | |
| Triton X-100 | inhibits at 1% | Geobacillus stearothermophilus | |
| Tween 20 | inhibits at 1% | Geobacillus stearothermophilus | |
| Zn2+ | 65% inhibition at 1 mM, 98.4% inhibition at 10 mM | Geobacillus stearothermophilus | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| tricaprylin + H2O | Geobacillus stearothermophilus | - |
dicaprylin + caprylate | - |
? | |
| tricaprylin + H2O | Geobacillus stearothermophilus P1 | - |
dicaprylin + caprylate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Geobacillus stearothermophilus | Q9L6D3 | - |
- |
| Geobacillus stearothermophilus P1 | Q9L6D3 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant enzyme, 18fold, to homogeneity in a one-step procedure | Geobacillus stearothermophilus |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| triacylglycerol + H2O = diacylglycerol + a carboxylate | enzyme contains an active site serine | Geobacillus stearothermophilus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 811 | - |
purified recombinant enzyme | Geobacillus stearothermophilus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl caprylate + H2O | - |
Geobacillus stearothermophilus | 4-nitrophenol + caprylate | - |
? | |
| 4-nitrophenyl caprylate + H2O | - |
Geobacillus stearothermophilus P1 | 4-nitrophenol + caprylate | - |
? | |
| additional information | substrate specificity, overview | Geobacillus stearothermophilus | ? | - |
? | |
| additional information | substrate specificity, overview | Geobacillus stearothermophilus P1 | ? | - |
? | |
| tricaprylin + H2O | - |
Geobacillus stearothermophilus | dicaprylin + caprylate | - |
? | |
| tricaprylin + H2O | best substrate | Geobacillus stearothermophilus | dicaprylin + caprylate | - |
? | |
| tricaprylin + H2O | - |
Geobacillus stearothermophilus P1 | dicaprylin + caprylate | - |
? | |
| tricaprylin + H2O | best substrate | Geobacillus stearothermophilus P1 | dicaprylin + caprylate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 43000-43209, recombinant enzyme, SDS-PAGE and mass spectrometry | Geobacillus stearothermophilus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| lipase | - |
Geobacillus stearothermophilus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 55 | - |
recombinant enzyme | Geobacillus stearothermophilus |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | 65 | - |
Geobacillus stearothermophilus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 65 | highly stable | Geobacillus stearothermophilus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | - |
recombinant enzyme | Geobacillus stearothermophilus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | 10 | - |
Geobacillus stearothermophilus |
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