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Literature summary for 3.1.1.3 extracted from

  • An, S.Y.; Kim, S.W.; Choi, Y.L.; Cho, Y.S.; Joo, W.H.; Lee, Y.C.
    Cloning, expression in Escherichia coli, and enzymatic properties of a lipase from Pseudomonas sp. SW-3 (2003), J. Microbiol., 41, 95-101.
No PubMed abstract available
Search for more literature for 3.1.1.3

Cloned(Commentary)

Cloned (Comment) Organism
cloning of lipase gene lipA and its activator gene lipB, immediately downstream of lipA, DNA and amino acid sequences determination and analysis, overexpression of the insoluble protein in Escherichia coli BL21(DE3) with coexpression of lipB resulting in an active enzyme, enzyme expressed without lipB is not active Pseudomonas sp.

Inhibitors

Inhibitors Comment Organism Structure
Co2+ 2 mM, pH 8.0 Pseudomonas sp.
Fe2+ 2 mM, pH 8.0 Pseudomonas sp.
Hg2+ 2 mM, pH 8.0 Pseudomonas sp.
Mg2+ 2 mM, pH 8.0 Pseudomonas sp.

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+
-
 Pseudomonas sp.
Na+
-
 Pseudomonas sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
31000
-
 x * 31000, recombinant refolded enzyme, SDS-PAGE Pseudomonas sp.

Organism

Organism UniProt Comment Textmining
Pseudomonas sp. Q9F0L9 lipase LipA or PLS, gene lipA
-
Pseudomonas sp. SW-3 Q9F0L9 lipase LipA or PLS, gene lipA
-

Purification (Commentary)

Purification (Comment) Organism
insoluble overexpressed recombinant enzyme from Escherichia coli, coeexpressed with activator LipB Pseudomonas sp.

Renatured (Commentary)

Renatured (Comment) Organism
insoluble recombinant enzyme is solubilized by 8 M urea, purified and refolded by removing urea in presence of Ca2+ Pseudomonas sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
36.3
-
 purified recombinant refolded enzyme, substrate 4-nitrophenyl palmitate Pseudomonas sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl caprate + H2O
-
 Pseudomonas sp. 4-nitrophenol + caprate
-
 ?
4-nitrophenyl caprate + H2O
-
 Pseudomonas sp. SW-3 4-nitrophenol + caprate
-
 ?
4-nitrophenyl laurate + H2O
-
 Pseudomonas sp. 4-nitrophenol + laurate
-
 ?
4-nitrophenyl laurate + H2O
-
 Pseudomonas sp. SW-3 4-nitrophenol + laurate
-
 ?
4-nitrophenyl myristate + H2O
-
 Pseudomonas sp. 4-nitrophenol + myristate
-
 ?
4-nitrophenyl myristate + H2O
-
 Pseudomonas sp. SW-3 4-nitrophenol + myristate
-
 ?
4-nitrophenyl palmitate + H2O best substrate Pseudomonas sp. 4-nitrophenol + palmitate
-
 ?
4-nitrophenyl palmitate + H2O best substrate Pseudomonas sp. SW-3 4-nitrophenol + palmitate
-
 ?
4-nitrophenyl stearate + H2O
-
 Pseudomonas sp. 4-nitrophenol + stearate
-
 ?
additional information enzyme shows a preference for long-chain fatty acid triacylglyceride substrates Pseudomonas sp. ?
-
 ?
additional information enzyme shows a preference for long-chain fatty acid triacylglyceride substrates Pseudomonas sp. SW-3 ?
-
 ?

Subunits

Subunits Comment Organism
? x * 31000, recombinant refolded enzyme, SDS-PAGE Pseudomonas sp.

Synonyms

Synonyms Comment Organism
lipase
-
 Pseudomonas sp.
More the enzyme belongs to the lipase family I.1 Pseudomonas sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
 Pseudomonas sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 stable, 90% remaining activity, 30 min Pseudomonas sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Pseudomonas sp.