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Literature summary for 3.1.1.3 extracted from

  • Sunna, A.; Hunter, L.; Hutton, C.A.; Bergquist, P.L.
    Biochemical characterization of a recombinant thermoalkalophilic lipase and assessment of its substrate enantioselectivity (2002), Enzyme Microb. Technol., 31, 472-476.
No PubMed abstract available
Search for more literature for 3.1.1.3

Cloned(Commentary)

Cloned (Comment) Organism
expression of LipA in Escherichia coli BL21-SI Bacillus sp. (in: Bacteria)

Organism

Organism UniProt Comment Textmining
Bacillus sp. (in: Bacteria)
-
 thermostable lipase LipA
-
Bacillus sp. (in: Bacteria) Tp10A.1
-
 thermostable lipase LipA
-

Purification (Commentary)

Purification (Comment) Organism
gene lipA, recombinant LipA from Escherichia coli Bacillus sp. (in: Bacteria)

Reaction

Reaction Comment Organism Reaction ID
triacylglycerol + H2O = diacylglycerol + a carboxylate enzyme shows high enantioselectivity on racemic substrates Bacillus sp. (in: Bacteria)
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(RS)-1-phenylethyl acetate + H2O enzyme hydrolyzes mainly the R-enantiomer, temperature-dependent, the enantioselectivity decreases with increasing temperature Bacillus sp. (in: Bacteria) (R)-1-phenylethanol + acetate + (R)-1-phenylethyl acetate
-
 ?
4-nitrophenyl butyrate + H2O low activity Bacillus sp. (in: Bacteria) 4-nitrophenol + butyrate
-
 ?
4-nitrophenyl butyrate + H2O low activity Bacillus sp. (in: Bacteria) Tp10A.1 4-nitrophenol + butyrate
-
 ?
4-nitrophenyl caprate + H2O best substrate Bacillus sp. (in: Bacteria) 4-nitrophenol + caprate
-
 ?
4-nitrophenyl caprylate + H2O best substrate Bacillus sp. (in: Bacteria) 4-nitrophenol + caprylate
-
 ?
4-nitrophenyl caprylate + H2O best substrate Bacillus sp. (in: Bacteria) Tp10A.1 4-nitrophenol + caprylate
-
 ?
4-nitrophenyl hexanoate + H2O
-
 Bacillus sp. (in: Bacteria) 4-nitrophenol + hexanoate
-
 ?
4-nitrophenyl hexanoate + H2O
-
 Bacillus sp. (in: Bacteria) Tp10A.1 4-nitrophenol + hexanoate
-
 ?
4-nitrophenyl laurate + H2O best substrate Bacillus sp. (in: Bacteria) 4-nitrophenol + laurate
-
 ?
4-nitrophenyl myristate + H2O
-
 Bacillus sp. (in: Bacteria) 4-nitrophenol + myristate
-
 ?
4-nitrophenyl palmitate + H2O low activity Bacillus sp. (in: Bacteria) 4-nitrophenol + palmitate
-
 ?
4-nitrophenyl palmitate + H2O low activity Bacillus sp. (in: Bacteria) Tp10A.1 4-nitrophenol + palmitate
-
 ?
4-nitrophenyl stearate + H2O
-
 Bacillus sp. (in: Bacteria) 4-nitrophenol + stearate
-
 ?
methyl(RS)-2-methyldecanoate + H2O enzyme hydrolyzes mainly the R-enantiomer, temperature-dependent, the enantioselectivity decreases with increasing temperature Bacillus sp. (in: Bacteria) (R)-2-methyldecanoic acid + methanol
-
 ?
additional information LipA has a preference for 4-nitrophenyl esters with longer fatty acid chains Bacillus sp. (in: Bacteria) ?
-
 ?
additional information LipA has a preference for 4-nitrophenyl esters with longer fatty acid chains Bacillus sp. (in: Bacteria) Tp10A.1 ?
-
 ?

Synonyms

Synonyms Comment Organism
lipase
-
 Bacillus sp. (in: Bacteria)

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
 recombinant enzyme Bacillus sp. (in: Bacteria)

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 recombinant enzyme Bacillus sp. (in: Bacteria)