Application | Comment | Organism |
---|---|---|
synthesis | the enzyme performs chemically selective hydrolysis, synthesis of optically active substances from achiral or racemic compounds | Pseudomonas mendocina |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ba2+ | 28% inhibition at 1 mM | Pseudomonas mendocina | |
Cu2+ | 52% inhibition at 1 mM | Pseudomonas mendocina | |
diethyldicarbonate | 65% inhibition at 0.1 mM, complete inhibition at 1 mM | Pseudomonas mendocina | |
EDTA | 38% inhibition at 1 mM | Pseudomonas mendocina | |
Fe3+ | 97% inhibition at 1 mM | Pseudomonas mendocina | |
Hg2+ | complete inhibition at 1 mM | Pseudomonas mendocina | |
Mn2+ | 17% inhibition at 1 mM | Pseudomonas mendocina | |
additional information | no inhibition by PMSF, no inhibition by Tween 20, Tween 80, and Triton X-100, negligible inhibition by Zn2+ | Pseudomonas mendocina | |
SDS | 95% inhibition at 0.4% | Pseudomonas mendocina |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | secretion to the culture medium | Pseudomonas mendocina | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | 20% activation at 1 mM | Pseudomonas mendocina | |
additional information | negligible stimulation by Na+ and Ca2+ at 1 mM | Pseudomonas mendocina |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
80000 | - |
x * 80000, SDS-PAGE | Pseudomonas mendocina |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Pseudomonas mendocina | Pseudomonas mendocina PK-12CS is able to grow on olive oil, soybean oil, tributyrin and triolein as sole carbon and energysource | ? | - |
? | |
additional information | Pseudomonas mendocina PK-12CS | Pseudomonas mendocina PK-12CS is able to grow on olive oil, soybean oil, tributyrin and triolein as sole carbon and energysource | ? | - |
? | |
tributyrin + H2O | Pseudomonas mendocina | - |
dibutyrin + butyrate | - |
? | |
tributyrin + H2O | Pseudomonas mendocina PK-12CS | - |
dibutyrin + butyrate | - |
? | |
triolein + H2O | Pseudomonas mendocina | - |
diolein + oleate | - |
? | |
triolein + H2O | Pseudomonas mendocina PK-12CS | - |
diolein + oleate | - |
? |
Organic Solvent | Comment | Organism |
---|---|---|
additional information | the enzyme shows good stability in water-miscible and immiscible solvents, overview | Pseudomonas mendocina |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas mendocina | - |
alkaline lipase | - |
Pseudomonas mendocina PK-12CS | - |
alkaline lipase | - |
Purification (Comment) | Organism |
---|---|
from culture supernatant to homogeneity, 241fold | Pseudomonas mendocina |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.28 | - |
purified enzyme | Pseudomonas mendocina |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | enzyme shows a preference for long-chain fatty acid triacylglyceride substrates and natural oils like olive oil, soybean oil, mustard oil, coconut oil, and best almond oil, substrate specificty overview | Pseudomonas mendocina | ? | - |
? | |
additional information | Pseudomonas mendocina PK-12CS is able to grow on olive oil, soybean oil, tributyrin and triolein as sole carbon and energysource | Pseudomonas mendocina | ? | - |
? | |
additional information | enzyme shows a preference for long-chain fatty acid triacylglyceride substrates and natural oils like olive oil, soybean oil, mustard oil, coconut oil, and best almond oil, substrate specificty overview | Pseudomonas mendocina PK-12CS | ? | - |
? | |
additional information | Pseudomonas mendocina PK-12CS is able to grow on olive oil, soybean oil, tributyrin and triolein as sole carbon and energysource | Pseudomonas mendocina PK-12CS | ? | - |
? | |
tributyrin + H2O | - |
Pseudomonas mendocina | dibutyrin + butyrate | - |
? | |
tributyrin + H2O | - |
Pseudomonas mendocina PK-12CS | dibutyrin + butyrate | - |
? | |
trilinolin + H2O | - |
Pseudomonas mendocina | dilinolin + linolate | - |
? | |
triolein + H2O | - |
Pseudomonas mendocina | diolein + oleate | - |
? | |
triolein + H2O | - |
Pseudomonas mendocina PK-12CS | diolein + oleate | - |
? | |
tripalmitin + H2O | - |
Pseudomonas mendocina | dipalmitin + palmitate | - |
? | |
tripalmitin + H2O | - |
Pseudomonas mendocina PK-12CS | dipalmitin + palmitate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 80000, SDS-PAGE | Pseudomonas mendocina |
Synonyms | Comment | Organism |
---|---|---|
alkaline lipase | - |
Pseudomonas mendocina |
PK-12CS lipase | - |
Pseudomonas mendocina |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
- |
Pseudomonas mendocina |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
22 | - |
room temperature, stable for more than one month | Pseudomonas mendocina |
45 | - |
1 h, loss of 50% activity | Pseudomonas mendocina |
57 | - |
1 h, loss of 75% activity | Pseudomonas mendocina |
60 | - |
half-life is 10 min | Pseudomonas mendocina |
65 | - |
half-life is 4 min | Pseudomonas mendocina |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Pseudomonas mendocina |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7.6 | 8.6 | 37°C, activity drops sharply below pH 7.6 and above pH 8.6 | Pseudomonas mendocina |