Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Thermus thermophilus |
Crystallization (Comment) | Organism |
---|---|
sitting drop vapor diffusion method, using 100 mM phosphate-citrate buffer pH 4.2, and 50% (w/v) 2-methyl-2,4-pentanediol | Thermus thermophilus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
N-substituted aminoacyl-tRNA + H2O | Thermus thermophilus | - |
N-substituted amino acid + tRNA | - |
? | |
N-substituted aminoacyl-tRNA + H2O | Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | - |
N-substituted amino acid + tRNA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermus thermophilus | - |
- |
- |
Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
HisTrap column chromatography, Ni-NTA agarose column chromatography, and Q Sepharose column chromatography | Thermus thermophilus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
N-substituted aminoacyl-tRNA + H2O | - |
Thermus thermophilus | N-substituted amino acid + tRNA | - |
? | |
N-substituted aminoacyl-tRNA + H2O | - |
Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | N-substituted amino acid + tRNA | - |
? |
Synonyms | Comment | Organism |
---|---|---|
peptidyl-tRNA hydrolase | - |
Thermus thermophilus |
PTH | - |
Thermus thermophilus |