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Literature summary for 3.1.1.29 extracted from

  • Matsumoto, A.; Shimizu, Y.; Takemoto, C.; Ueda, T.; Uchiumi, T.; Ito, K.
    Crystallization and preliminary X-ray analysis of peptidyl-tRNA hydrolase from Thermus thermophilus HB8 (2013), Acta Crystallogr. Sect. F, 69, 332-335.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Thermus thermophilus

Crystallization (Commentary)

Crystallization (Comment) Organism
sitting drop vapor diffusion method, using 100 mM phosphate-citrate buffer pH 4.2, and 50% (w/v) 2-methyl-2,4-pentanediol Thermus thermophilus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
N-substituted aminoacyl-tRNA + H2O Thermus thermophilus
-
N-substituted amino acid + tRNA
-
?
N-substituted aminoacyl-tRNA + H2O Thermus thermophilus HB8 / ATCC 27634 / DSM 579
-
N-substituted amino acid + tRNA
-
?

Organism

Organism UniProt Comment Textmining
Thermus thermophilus
-
-
-
Thermus thermophilus HB8 / ATCC 27634 / DSM 579
-
-
-

Purification (Commentary)

Purification (Comment) Organism
HisTrap column chromatography, Ni-NTA agarose column chromatography, and Q Sepharose column chromatography Thermus thermophilus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N-substituted aminoacyl-tRNA + H2O
-
Thermus thermophilus N-substituted amino acid + tRNA
-
?
N-substituted aminoacyl-tRNA + H2O
-
Thermus thermophilus HB8 / ATCC 27634 / DSM 579 N-substituted amino acid + tRNA
-
?

Synonyms

Synonyms Comment Organism
peptidyl-tRNA hydrolase
-
Thermus thermophilus
PTH
-
Thermus thermophilus