Crystallization (Comment) | Organism |
---|---|
crystallization using polyethylene glycol as precipitant and isopropanol as additive, crystal structure at 1.2 A | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
D93A | turnover-number is 0.1% of the turnover-number for wild-type enzyme, Km-value for diacetyl-Lys-tRNALys is 1.67fold higher than the Km-value for the wild-type enzyme | Escherichia coli |
F66A | turnover-number is 26% of the turnover-number for wild-type enzyme, Km-value for diacetyl-Lys-tRNALys is 1.15fold higher than the Km-value for the wild-type enzyme | Escherichia coli |
H113A | turnover-number is 33% of the turnover-number for wild-type enzyme, Km-value for diacetyl-Lys-tRNALys is 1.46fold higher than the Km-value for the wild-type enzyme | Escherichia coli |
H20A | no activity measurable with diacetyl-Lys-tRNALys | Escherichia coli |
K142A | turnover-number is 24% of the turnover-number for wild-type enzyme, Km-value for diacetyl-Lys-tRNALys is 4fold higher than the Km-value for the wild-type enzyme | Escherichia coli |
M67A | turnover-number is 4.7% of the turnover-number for wild-type enzyme, Km-value for diacetyl-Lys-tRNALys is 70% of the Km-value for the wild-type enzyme | Escherichia coli |
N10A | turnover-number is 0.7% of the turnover-number for wild-type enzyme, Km-value for diacetyl-Lys-tRNALys is 1.1fold higher than the Km-value for the wild-type enzyme | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0041 | - |
diacetyl-Lys-tRNALys | pH 7.5, 28°C, mutant enzyme M67A | Escherichia coli | |
0.006 | - |
diacetyl-Lys-tRNALys | pH 7.5, 28°C, wild-type enzyme | Escherichia coli | |
0.0066 | - |
diacetyl-Lys-tRNALys | pH 7.5, 28°C, mutant enzyme N10A | Escherichia coli | |
0.0069 | - |
diacetyl-Lys-tRNALys | pH 7.5, 28°C, mutant enzyme F66A | Escherichia coli | |
0.0088 | - |
diacetyl-Lys-tRNALys | pH 7.5, 28°C, mutant enzyme H113A | Escherichia coli | |
0.01 | - |
diacetyl-Lys-tRNALys | pH 7.5, 28°C, mutant enzyme D93A | Escherichia coli | |
0.024 | - |
diacetyl-Lys-tRNALys | pH 7.5, 28°C, 5'-dephosphoylated diacetyl-Lys-tRNALys, wild-type enzyme | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P0A7D1 | wild-type and mutant enzymes N10A, H20A, M67A, F66A, D93A, H113A, K142A | - |
Purification (Comment) | Organism |
---|---|
wild-type and mutant enzymes N10A, H20A, M67A, F66A, D93A, H113A, K142A | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
diacetyl-Lys-tRNALys + H2O | - |
Escherichia coli | diacetyl-Lys + tRNA | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.028 | - |
diacetyl-Lys-tRNALys | pH 7.5, 28°C, mutant enzyme N10A | Escherichia coli | |
0.05 | - |
diacetyl-Lys-tRNALys | pH 7.5, 28°C, mutant enzyme D93A | Escherichia coli | |
0.17 | - |
diacetyl-Lys-tRNALys | pH 7.5, 28°C, mutant enzyme M67A | Escherichia coli | |
0.85 | - |
diacetyl-Lys-tRNALys | pH 7.5, 28°C, 5'-dephosphoylated diacetyl-Lys-tRNALys, wild-type enzyme | Escherichia coli | |
0.9 | - |
diacetyl-Lys-tRNALys | pH 7.5, 28°C, mutant enzyme F66A | Escherichia coli | |
1.2 | - |
diacetyl-Lys-tRNALys | pH 7.5, 28°C, mutant enzyme H113A | Escherichia coli | |
3.6 | - |
diacetyl-Lys-tRNALys | pH 7.5, 28°C, wild-type enzyme | Escherichia coli |