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Literature summary for 3.1.1.29 extracted from

  • Schmitt, E.; Mechulam, Y.; Fromant, M.; Plateau, P.; Blanquet, S.
    Crystal structure at 1.2 A resolution and active site mapping of Escherichia coli peptidyl-tRNA hydrolase (1997), EMBO J., 16, 4760-4769.
    View publication on PubMedView publication on EuropePMC

Crystallization (Commentary)

Crystallization (Comment) Organism
crystallization using polyethylene glycol as precipitant and isopropanol as additive, crystal structure at 1.2 A Escherichia coli

Protein Variants

Protein Variants Comment Organism
D93A turnover-number is 0.1% of the turnover-number for wild-type enzyme, Km-value for diacetyl-Lys-tRNALys is 1.67fold higher than the Km-value for the wild-type enzyme Escherichia coli
F66A turnover-number is 26% of the turnover-number for wild-type enzyme, Km-value for diacetyl-Lys-tRNALys is 1.15fold higher than the Km-value for the wild-type enzyme Escherichia coli
H113A turnover-number is 33% of the turnover-number for wild-type enzyme, Km-value for diacetyl-Lys-tRNALys is 1.46fold higher than the Km-value for the wild-type enzyme Escherichia coli
H20A no activity measurable with diacetyl-Lys-tRNALys Escherichia coli
K142A turnover-number is 24% of the turnover-number for wild-type enzyme, Km-value for diacetyl-Lys-tRNALys is 4fold higher than the Km-value for the wild-type enzyme Escherichia coli
M67A turnover-number is 4.7% of the turnover-number for wild-type enzyme, Km-value for diacetyl-Lys-tRNALys is 70% of the Km-value for the wild-type enzyme Escherichia coli
N10A turnover-number is 0.7% of the turnover-number for wild-type enzyme, Km-value for diacetyl-Lys-tRNALys is 1.1fold higher than the Km-value for the wild-type enzyme Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0041
-
diacetyl-Lys-tRNALys pH 7.5, 28°C, mutant enzyme M67A Escherichia coli
0.006
-
diacetyl-Lys-tRNALys pH 7.5, 28°C, wild-type enzyme Escherichia coli
0.0066
-
diacetyl-Lys-tRNALys pH 7.5, 28°C, mutant enzyme N10A Escherichia coli
0.0069
-
diacetyl-Lys-tRNALys pH 7.5, 28°C, mutant enzyme F66A Escherichia coli
0.0088
-
diacetyl-Lys-tRNALys pH 7.5, 28°C, mutant enzyme H113A Escherichia coli
0.01
-
diacetyl-Lys-tRNALys pH 7.5, 28°C, mutant enzyme D93A Escherichia coli
0.024
-
diacetyl-Lys-tRNALys pH 7.5, 28°C, 5'-dephosphoylated diacetyl-Lys-tRNALys, wild-type enzyme Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli P0A7D1 wild-type and mutant enzymes N10A, H20A, M67A, F66A, D93A, H113A, K142A
-

Purification (Commentary)

Purification (Comment) Organism
wild-type and mutant enzymes N10A, H20A, M67A, F66A, D93A, H113A, K142A Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
diacetyl-Lys-tRNALys + H2O
-
Escherichia coli diacetyl-Lys + tRNA
-
?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.028
-
diacetyl-Lys-tRNALys pH 7.5, 28°C, mutant enzyme N10A Escherichia coli
0.05
-
diacetyl-Lys-tRNALys pH 7.5, 28°C, mutant enzyme D93A Escherichia coli
0.17
-
diacetyl-Lys-tRNALys pH 7.5, 28°C, mutant enzyme M67A Escherichia coli
0.85
-
diacetyl-Lys-tRNALys pH 7.5, 28°C, 5'-dephosphoylated diacetyl-Lys-tRNALys, wild-type enzyme Escherichia coli
0.9
-
diacetyl-Lys-tRNALys pH 7.5, 28°C, mutant enzyme F66A Escherichia coli
1.2
-
diacetyl-Lys-tRNALys pH 7.5, 28°C, mutant enzyme H113A Escherichia coli
3.6
-
diacetyl-Lys-tRNALys pH 7.5, 28°C, wild-type enzyme Escherichia coli