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Literature summary for 3.1.1.29 extracted from

  • Dutka, S.; Meinnel, T.; Lazennec, C.; Mechulam, Y.; Blanquet, S.
    Role of the 1-72 base pair in tRNAs for the activity of Escherichia coli peptidyl-tRNA hydrolase (1993), Nucleic Acids Res., 21, 4025-4030.
    View publication on PubMedView publication on EuropePMC

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information effect of mutations altering the 1-72 pair of E. coli tRNAMetf on the Km-value Escherichia coli
0.0022
-
N-acetyl-Met-tRNA pH 7.0, 37°C Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ the enzyme almost completely inactive in absence of divalent cations. Mn2+ is partly effective, optimal concentration is about 0.2-0.5 mM Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
construction of a bacterial strain overexpressing the enzyme
-

Purification (Commentary)

Purification (Comment) Organism
-
Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Met-tRNAMet + H2O
-
Escherichia coli Met + tRNAMet
-
?
additional information derivatives of tRNAMetf with various combinations of bases at position 1 and 72 in the acceptor stem have been produced, aminoacylated and chemically acetylated. TrNAmetd derivatives with either C1A72, c1C72, U1G72, U1C72 or A1C72 behave as poor substrates of the enzyme compared to those with C1G72, U1A72, G1C72, A1U72 or G1U72 Escherichia coli ?
-
?
N-acetyl-Met-tRNA + H2O
-
Escherichia coli N-acetyl-Met + tRNA
-
?
Val-tRNAVal + H2O
-
Escherichia coli Val + tRNAVal
-
?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
additional information
-
additional information effect of mutations altering the 1-72 pair of E. coli tRNAMetf on the turnover-number Escherichia coli
0.7
-
N-acetyl-Met-tRNA pH 7.0, 37°C Escherichia coli

pI Value

Organism Comment pI Value Maximum pI Value
Escherichia coli isoelectric focusing
-
10