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Literature summary for 3.1.1.29 extracted from

  • Gallego, M.E.; Heredia, C.F.
    Purification and properties of an inducible aminoacyl-tRNA hydrolase from Artemia larvae (1982), Biochim. Biophys. Acta, 696, 57-65.
No PubMed abstract available
Search for more literature for 3.1.1.29

Inhibitors

Inhibitors Comment Organism Structure
deacetylated tRNA
-
 Artemia sp.
EDTA 5 mM, complete inhibition Artemia sp.

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ the enzyme is maximally active in presence of divalent cations, Mg2+ or Mn2+. Maximal activity with 20 mM Mg2+ Artemia sp.
Mn2+ the enzyme is maximally active in presence of divalent cations, Mg2+ or Mn2+. Maximal activity with 2.5 mM, 50% of the activation with Mg2+ Artemia sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
55000
-
 gel filtration Artemia sp.

Organism

Organism UniProt Comment Textmining
Artemia sp.
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Artemia sp.

Source Tissue

Source Tissue Comment Organism Textmining
larva
-
 Artemia sp.
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0149
-
-
 Artemia sp.

Storage Stability

Storage Stability Organism
-10°C, 50% glycerol, purified enzyme is stable for several months Artemia sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Glu-tRNA + H2O
-
 Artemia sp. Glu + tRNA
-
 ?
Gly-tRNA + H2O
-
 Artemia sp. Gly + tRNA
-
 ?
Leu-tRNA + H2O
-
 Artemia sp. Leu + tRNA
-
 ?
Lys-tRNA + H2O
-
 Artemia sp. Lys + tRNA
-
 ?
N-acetyl-Glu-tRNA + H2O
-
 Artemia sp. N-acetyl-Glu + tRNA
-
 ?
N-acetyl-Leu-tRNA + H2O
-
 Artemia sp. N-acetyl-Leu + tRNA
-
 ?
N-acetyl-Lys-tRNA + H2O
-
 Artemia sp. N-acetyl-Lys + tRNA
-
 ?
N-acetyl-Phe-tRNA + H2O
-
 Artemia sp. N-acetyl-Phe + tRNA
-
 ?
N-acetyl-Ser-tRNA + H2O
-
 Artemia sp. N-acetyl-Ser + tRNA
-
 ?
N-acetyl-Val-tRNA + H2O
-
 Artemia sp. N-acetyl-Val + tRNA
-
 ?
Phe-tRNA + H2O
-
 Artemia sp. Phe + tRNA
-
 ?
Ser-tRNA + H2O
-
 Artemia sp. Ser + tRNA
-
 ?
Val-tRNA + H2O
-
 Artemia sp. Val + tRNA
-
 ?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 10 min, 85% loss of activity Artemia sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7 7.5
-
 Artemia sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
6 7.5 4°C, 24 h, stable Artemia sp.