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Literature summary for 3.1.1.29 extracted from
- Peptidyl-tRNA hydrolase from Sulfolobus solfataricus (2003), Nucleic Acids Res., 31, 3227-3235.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| spermidine | 0.1 mM spermidine HCl, 73fold activation | Saccharolobus solfataricus |  |
| spermidine-HCl | 0.1 mM, 73fold activation | Saccharolobus solfataricus | |
| spermidine-HCl | activates about 80fold, optimally at 0.1 mM | Saccharolobus solfataricus | |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Saccharolobus solfataricus |
| gene SS00175, DNA and amino acid sequence determination and analysis, complementation of an enzyme-deficient Escherichia coli mutant strain, and of two Saccharomyces cerevisiae gene YHR189w or YBL057c disruption mutants, overview, expression of PTH in Escherichia coli strain XL 1-Blue | Saccharolobus solfataricus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| non-esterified tRNALys | - |
Saccharolobus solfataricus | |
| tRNA | unfractionated Escherichia coli tRNA; unfractionated from Escherichia coli | Saccharolobus solfataricus | |
| tRNA-formyl-methionine | from Escherichia coli | Saccharolobus solfataricus | |
| tRNA-lysine | from Escherichia coli | Saccharolobus solfataricus | |
| tRNAfMet | tRNAfMet from Escherichia coli | Saccharolobus solfataricus | |
| tRNALys | tRNALys from Escherichia coli | Saccharolobus solfataricus | |
| Uncharged tRNA | e.g. from Escherichia coli, at concentrations of 0.01 mM or above | Saccharolobus solfataricus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0000028 | - |
diacetyl-Lys-tRNALys | pH 7.5, 50°C, dephosphorylated diacetyl-Lys-tRNALys | Saccharolobus solfataricus | |
| 0.0000028 | - |
diacetyl-lysyl-tRNALys | dephosphorylated substrate, pH 7.5, 50°C, recombinant enzyme | Saccharolobus solfataricus | |
| 0.000003 | - |
dephosphorylated diacyl-lysine-tRNA | 50°C, pH 7.5 | Saccharolobus solfataricus | |
| 0.000011 | - |
diacetyl-Lys-tRNALys | pH 7.5, 50°C | Saccharolobus solfataricus | |
| 0.000011 | - |
diacetyl-lysine-tRNA | 50°C, pH 7.5 | Saccharolobus solfataricus | |
| 0.000011 | - |
diacetyl-lysyl-tRNALys | pH 7.5, 50°C, recombinant enzyme | Saccharolobus solfataricus | |
| 0.000012 | - |
formyl-Met-tRNAfMet | pH 7.5, 50°C | Saccharolobus solfataricus | |
| 0.000012 | - |
formyl-methionyl-tRNA | 50°C, pH 7.5 | Saccharolobus solfataricus | |
| 0.000012 | - |
formyl-methionyl-tRNAfMet | pH 7.5, 50°C, recombinant enzyme | Saccharolobus solfataricus | |
| 0.000016 | - |
acetyl-His-tRNAHis | pH 7.5, 50°C | Saccharolobus solfataricus | |
| 0.000016 | - |
acetyl-histidyl-tRNA | 50°C, pH 7.5 | Saccharolobus solfataricus | |
| 0.000016 | - |
acetyl-histidyl-tRNAHis | pH 7.5, 50°C, recombinant enzyme | Saccharolobus solfataricus | |
| 0.00003 | - |
dephosphorylated formyl-methioninyl-tRNA | 50°C, pH 7.5 | Saccharolobus solfataricus | |
| 0.00003 | - |
dephosphorylated formyl-Met-tRNAfMet | pH 7.5, 50°C, dephosphorylated formyl-Met-tRNAfMet | Saccharolobus solfataricus | |
| 0.00003 | - |
formyl-methionyl-tRNAfMet | dephosphorylated substrate, pH 7.5, 50°C, recombinant enzyme | Saccharolobus solfataricus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| K+ | 0.3 mM, 77fold activation | Saccharolobus solfataricus | |
| KCl | 300 mM, 77fold activation | Saccharolobus solfataricus | |
| KCl | activates about 80fold, optimally at 300 mM | Saccharolobus solfataricus | |
| Mg2+ | 40 mM MgCl2, about 88fold activation | Saccharolobus solfataricus | |
| Mg2+ | activates about 80fold, optimally at 40 mM | Saccharolobus solfataricus | |
| MgCl2 | 40 mM, approx. 88fold activation | Saccharolobus solfataricus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 13100 | - |
2 * 13100, deduced from nucleotide sequence | Saccharolobus solfataricus |
| 15000 | - |
x * 15000, SDS-PAGE | Saccharolobus solfataricus |
| 15000 | - |
2 * 15000, recombinant enzyme, SDS-PAGE | Saccharolobus solfataricus |
| 25000 | - |
gel filtration | Saccharolobus solfataricus |
| 25000 | - |
recombinant enzyme, gel filtration | Saccharolobus solfataricus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| diacetyl-lysyl-tRNALys + H2O | Saccharolobus solfataricus | - |
diacetyl-lysine + tRNALys | - |
? | |
| N-substituted aminoacyl-tRNA + H2O | Escherichia coli | - |
N-substituted amino acid + tRNA | - |
? | |
| N-substituted aminoacyl-tRNA + H2O | Saccharolobus solfataricus | - |
N-substituted amino acid + tRNA | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
| Saccharolobus solfataricus | - |
- |
- |
| Saccharolobus solfataricus | - |
gene SSO0175 | - |
| Saccharomyces cerevisiae | - |
YHR189w and YBL057c gene products | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Escherichia coli |
- |
Saccharolobus solfataricus |
| recombinant PTH, heat, ammonium sulfate, Superdex 75, HI-Propyl, SP-Sepharose | Saccharolobus solfataricus |
| recomninant PTH from Escherichia coli strain XL 1-Blue 8500fold by ultracentrifugation, heat treatment, ammonium sulfate fractionation, gel filtration, hydrophobic interaction chromatography, and ion exchange chromatography to homogeneity | Saccharolobus solfataricus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Saccharolobus solfataricus |
| 0.078 | - |
substrate D-tyrosyl-tRNA, 37°C, pH 7.5 | Saccharolobus solfataricus |
| 0.15 | - |
substrae D-tyrosyl-tRNA | Escherichia coli |
| 4.14 | - |
- |
Saccharolobus solfataricus |
| 4.14 | - |
purified recombinant enzyme | Saccharolobus solfataricus |
| 30 | - |
substrate diacetyl-lysine-tRNA | Escherichia coli |
| 31.8 | - |
substrate diacetyl-lysine tRNA, 37°C, pH 7.5 | Saccharolobus solfataricus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5'-dephosphorylated N,N-diacetyl-L-lysyl-tRNALys + H2O | - |
Saccharolobus solfataricus | N,N-diacetyl-L-lysine + 5'-dephosphorylated tRNALys | - |
? | |
| 5'-dephosphorylated N-formyl-L-methionyl-tRNA + H2O | - |
Saccharolobus solfataricus | N-formyl-L-methionine + 5'-dephosphorylated tRNA | - |
? | |
| acetyl-histidine-tRNA + H2O | - |
Saccharolobus solfataricus | acetyl-histidine + tRNA | - |
? | |
| acetyl-histidyl-tRNAHis + H2O | - |
Saccharolobus solfataricus | acetyl-histidine + tRNAHis | - |
? | |
| D-tyrosine-tRNA + H2O | - |
Escherichia coli | D-tyrosine + tRNA | - |
? | |
| D-tyrosine-tRNA + H2O | - |
Saccharolobus solfataricus | D-tyrosine + tRNA | - |
? | |
| dephosphorylated diacyl-lysine-tRNA + H2O | - |
Saccharolobus solfataricus | diacyl-lysine + dephosphorylated tRNA | - |
? | |
| dephosphorylated formyl-Met-tRNAfMet + H2O | - |
Saccharolobus solfataricus | formyl-Met + dephosphorylated tRNAfMet | - |
? | |
| dephosphorylated formyl-methioninyl-tRNA + H2O | - |
Saccharolobus solfataricus | formyl-methionine + dephosphorylated tRNA | - |
? | |
| diacetyl-Lys-tRNALys + H2O | - |
Saccharomyces cerevisiae | diacetyl-Lys + tRNA | - |
? | |
| diacetyl-Lys-tRNALys + H2O | diacetyl-Lys-tRNALys from E. coli | Saccharolobus solfataricus | diacetyl-Lys + tRNA | - |
? | |
| diacetyl-lysine-tRNA + H2O | - |
Escherichia coli | diacetyl-lysine + tRNA | - |
? | |
| diacetyl-lysine-tRNA + H2O | - |
Saccharolobus solfataricus | diacetyl-lysine + tRNA | - |
? | |
| diacetyl-lysyl-tRNALys + H2O | - |
Saccharolobus solfataricus | diacetyl-lysine + tRNALys | - |
? | |
| diacetyl-lysyl-tRNALys + H2O | Escherichia coli diacetyl-lysyl-tRNALys, phosphorylated and dephosphorylated substrate | Saccharolobus solfataricus | diacetyl-lysine + tRNALys | - |
? | |
| formyl-Met-tRNAfMet + H2O | - |
Saccharolobus solfataricus | formyl-Met + tRNAfMet | - |
? | |
| formyl-methionine-tRNA + H2O | - |
Saccharolobus solfataricus | formyl-methionine + tRNA | - |
? | |
| formyl-methioninyl-tRNA + H2O | - |
Saccharolobus solfataricus | formyl-methionine + tRNA | - |
? | |
| formyl-methionyl-tRNAfMet + H2O | Escherichia coli formyl-methionyltRNAfMet, phosphorylated and dephosphorylated substrate | Saccharolobus solfataricus | formyl-methionine + tRNAfMet | - |
? | |
| additional information | the enzyme is involved in the recycling of peptidyl-tRNA, it shows poor D-aminoacyl-tRNA hydrolysis | Saccharolobus solfataricus | ? | - |
? | |
| N-acetyl-His-tRNA + H2O | - |
Saccharolobus solfataricus | N-acetyl-His + tRNA | - |
? | |
| N-substituted aminoacyl-tRNA + H2O | - |
Escherichia coli | N-substituted amino acid + tRNA | - |
? | |
| N-substituted aminoacyl-tRNA + H2O | - |
Saccharolobus solfataricus | N-substituted amino acid + tRNA | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 15000, SDS-PAGE | Saccharolobus solfataricus |
| dimer | 2 * 13100, deduced from nucleotide sequence | Saccharolobus solfataricus |
| dimer | 2 * 15000, recombinant enzyme, SDS-PAGE | Saccharolobus solfataricus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| peptidyl-tRNA hydrolase | - |
Saccharolobus solfataricus |
| PTH | - |
Escherichia coli |
| PTH | - |
Saccharolobus solfataricus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
assay at | Saccharolobus solfataricus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.86 | - |
diacetyl-Lys-tRNALys | pH 7.5, 50°C, dephosphorylated diacetyl-Lys-tRNALys | Saccharolobus solfataricus | |
| 0.86 | - |
dephosphorylated diacyl-lysine-tRNA | 50°C, pH 7.5 | Saccharolobus solfataricus | |
| 0.86 | - |
diacetyl-lysyl-tRNALys | dephosphorylated substrate, pH 7.5, 50°C, recombinant enzyme | Saccharolobus solfataricus | |
| 1.8 | - |
diacetyl-Lys-tRNALys | pH 7.5, 50°C | Saccharolobus solfataricus | |
| 1.8 | - |
diacetyl-lysine-tRNA | 50°C, pH 7.5 | Saccharolobus solfataricus | |
| 1.8 | - |
diacetyl-lysyl-tRNALys | pH 7.5, 50°C, recombinant enzyme | Saccharolobus solfataricus | |
| 3 | - |
formyl-Met-tRNAfMet | pH 7.5, 50°C | Saccharolobus solfataricus | |
| 3 | - |
formyl-methionyl-tRNA | 50°C, pH 7.5 | Saccharolobus solfataricus | |
| 3 | - |
dephosphorylated formyl-methioninyl-tRNA | 50°C, pH 7.5 | Saccharolobus solfataricus | |
| 3 | - |
formyl-methioninyl-tRNA | 50°C, pH 7.5 | Saccharolobus solfataricus | |
| 3 | - |
formyl-methionyl-tRNAfMet | phosphorylated and dephosphorylated substrate, pH 7.5, 50°C, recombinant enzyme | Saccharolobus solfataricus | |
| 3.4 | - |
acetyl-His-tRNA | pH 7.5, 50°C | Saccharolobus solfataricus | |
| 3.4 | - |
acetyl-histidyl-tRNA | 50°C, pH 7.5 | Saccharolobus solfataricus | |
| 3.4 | - |
acetyl-histidyl-tRNAHis | pH 7.5, 50°C, recombinant enzyme | Saccharolobus solfataricus | |
| 6.08 | - |
dephosphorylated diacyl-lysine-tRNA | 50°C, pH 7.5 | Saccharolobus solfataricus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Saccharolobus solfataricus |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00005 | - |
tRNALys | pH 7.5, 50°C, tRNALys from Escherichia coli | Saccharolobus solfataricus | |
| 0.00005 | - |
tRNA-lysine | tRNA-lysine from Escherichia coli, 50°C, pH 7.5 | Saccharolobus solfataricus | |
| 0.00005 | - |
non-esterified tRNALys | pH 7.5, 50°C, recombinant enzyme | Saccharolobus solfataricus | |
| 0.000095 | - |
tRNA | pH 7.5, 50°C, unfractionated Escherichia coli tRNA | Saccharolobus solfataricus | |
| 0.000095 | - |
tRNA | unfractionated tRNA from Escherichia coli, 50°C, pH 7.5 | Saccharolobus solfataricus | |
| 0.0001 | - |
tRNA-formyl-methionine | tRNA-ormyl-methionine from Escherichia coli, 50°C, pH 7.5 | Saccharolobus solfataricus | |
| 0.0001 | - |
Uncharged tRNA | pH 7.5, 50°C, recombinant enzyme | Saccharolobus solfataricus | |
| 0.00011 | - |
tRNAformylMet | pH 7.5, 50°C, tRNAformylMet from Escherichia coli | Saccharolobus solfataricus | |
| 0.00011 | - |
tRNAfMet | pH 7.5, 50°C, recombinant enzyme | Saccharolobus solfataricus | |
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