Literature summary for 3.1.1.27 extracted from

Matsuda, S.; Yokochi, N.; Yoshikane, Y.; Kobayashi, J.; Huy, C.N.; Baba, S.; Kuramitsu, S.; Mikami, B.; Yagi, T.
Crystallization and preliminary X-ray analysis of 4-pyridoxolactonase from Mesorhizobium loti (2009), Acta Crystallogr. Sect. F, 65, 886-889.

Search for more literature for 3.1.1.27

Cloned(Commentary)

Cloned (Comment)	Organism
overexpressed in Escherichia coli BL21 (DE3) cells harbouring plasmid pET21a6805	Mesorhizobium loti

Crystallization (Commentary)

Crystallization (Comment)	Organism
by the sitting-drop vapour-diffusion method, at 4°C. Free enzyme to 2.0 A resolution, belongs to the monoclinic space group C2, with unit-cell parameters a = 77.93, b = 38.88, c = 81.60 A, beta = 117.33. The 5-pyridoxolactone-bound enzyme to 1.9 A resolution, belongs to the monoclinic space group C2, with unit-cell parameters a = 86.24, b = 39.35, c = 82.68 A, beta = 118.02	Mesorhizobium loti

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
31000	-	x * 31000, SDS-PAGE	Mesorhizobium loti

Organism

Organism	UniProt	Comment	Textmining
Mesorhizobium loti	-	-	-
Mesorhizobium loti MAFF303099	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant enzyme purified sequentially by gel filtration and ammonium sulfate precipitation	Mesorhizobium loti

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
4-pyridoxolactone + H2O	-	Mesorhizobium loti	4-pyridoxate	-	?
4-pyridoxolactone + H2O	-	Mesorhizobium loti MAFF303099	4-pyridoxate	-	?

Subunits

Subunits	Comment	Organism
?	x * 31000, SDS-PAGE	Mesorhizobium loti

Synonyms

Synonyms	Comment	Organism
4-pyridoxolactonase	-	Mesorhizobium loti

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Release 2023.1 (January 2023)