BRENDA - Enzyme Database
show all sequences of 3.1.1.26

Purification and properties of a lipolytic acyl-hydrolase from potato leaves

Matsuda, H.; Hirayama, O.; Biochim. Biophys. Acta 573, 155-165 (1979)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
EDTA
113% activity at 0.1 mM, 140% activity at 10 mM
Solanum tuberosum
Inhibitors
Inhibitors
Commentary
Organism
Structure
CaCl2
no inhibition at 0.1 mM, 13% inhibition at 1 mM, 47% inhibition at 10 mM
Solanum tuberosum
MgCl2
53% inhibition at 10 mM
Solanum tuberosum
NaCl
84% inhibition at 50 mM
Solanum tuberosum
SDS
0.1% complete inhibition
Solanum tuberosum
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.26
-
monogalactosyldiacylglycerol
-
Solanum tuberosum
0.54
-
phosphatidylcholine
-
Solanum tuberosum
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
110000
-
SDS-PAGE, gel filtration
Solanum tuberosum
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
digalactosyldiacylglycerol + H2O
Solanum tuberosum
-
?
-
-
-
monogalactosyldiacylglycerol + H2O
Solanum tuberosum
-
?
-
-
-
Organic Solvent Stability
Organic Solvent
Commentary
Organism
Acetone
85% inhibition at 10%
Solanum tuberosum
Ethanol
98% inhibition at 10%
Solanum tuberosum
ether
96% inhibition at 10%
Solanum tuberosum
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Solanum tuberosum
-
L. cv. Benimaru
-
Purification (Commentary)
Commentary
Organism
520fold
Solanum tuberosum
Source Tissue
Source Tissue
Commentary
Organism
Textmining
leaf
-
Solanum tuberosum
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2,3-di-O-acyl-1-O-(beta-D-galactosyl)-D-glycerol + H2O
-
94293
Solanum tuberosum
?
-
-
-
?
digalactosyldiacylglycerol + H2O
44% of the activity with monogalactosyldiacylglycerol
94293
Solanum tuberosum
digalactosylglycerol + fatty acid
-
-
-
?
digalactosyldiacylglycerol + H2O
-
94293
Solanum tuberosum
?
-
-
-
-
digalactosylmonoacylglycerol + H2O
146% of the activity with monogalactosyldiacylglycerol
94293
Solanum tuberosum
digalactosylglycerol + fatty acid
-
-
-
?
lysophosphatidylcholine + H2O
26% of the activity with monogalactosyldiacylglycerol
94293
Solanum tuberosum
?
-
-
-
?
lysophosphatidylethanolamine + H2O
6% of the activity with monogalactosyldiacylglycerol
94293
Solanum tuberosum
?
-
-
-
?
lysophosphatidylglycerol + H2O
89% of the activity with monogalactosyldiacylglycerol
94293
Solanum tuberosum
?
-
-
-
?
monogalactosyldiacylglycerol + H2O
-
94293
Solanum tuberosum
galactosylglycerol + fatty acid
-
-
-
?
monogalactosyldiacylglycerol + H2O
-
94293
Solanum tuberosum
?
-
-
-
-
monogalactosylmonoacylglycerol + H2O
394% of the activity with monogalactosyldiacylglycerol
94293
Solanum tuberosum
monogalactosylglycerol + fatty acid
-
-
-
?
phosphatidic acid + H2O
8% of the activity with monogalactosyldiacylglycerol
94293
Solanum tuberosum
?
-
-
-
?
phosphatidylcholine + H2O
14% of the activity with monogalactosyldiacylglycerol
94293
Solanum tuberosum
?
-
-
-
?
phosphatidylethanolamine + H2O
1% of the activity with monogalactosyldiacylglycerol
94293
Solanum tuberosum
?
-
-
-
?
phosphatidylglycerol + H2O
77% of the activity with monogalactosyldiacylglycerol
94293
Solanum tuberosum
?
-
-
-
?
sulfoquinovosyldiaclglycerol + H2O
43% of the activity with monogalactosyldiacylglycerol
94293
Solanum tuberosum
?
-
-
-
?
triacylglycerol + H2O
below 0.1% of the activity with monogalactosyldiacylglycerol
94293
Solanum tuberosum
?
-
-
-
?
Temperature Optimum [C]
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
30
-
phosphatidylcholine as substrate
Solanum tuberosum
35
-
monogalactosyldiacylglycerol as substrate
Solanum tuberosum
Temperature Stability [C]
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
60
-
completely inactivated after 10 min at pH 8.0, 70% of its activity retained at pH 5.5
Solanum tuberosum
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
5.5
-
monogalactosyldiacylglycerol as substrate, at 35C, shift to pH 6.0 in the presence of Triton X-100
Solanum tuberosum
6
-
monogalactosyldiacylglycerol as substrate, at 35C, shift from pH 5.5 in the presence of Triton X-100
Solanum tuberosum
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
EDTA
113% activity at 0.1 mM, 140% activity at 10 mM
Solanum tuberosum
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
CaCl2
no inhibition at 0.1 mM, 13% inhibition at 1 mM, 47% inhibition at 10 mM
Solanum tuberosum
MgCl2
53% inhibition at 10 mM
Solanum tuberosum
NaCl
84% inhibition at 50 mM
Solanum tuberosum
SDS
0.1% complete inhibition
Solanum tuberosum
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.26
-
monogalactosyldiacylglycerol
-
Solanum tuberosum
0.54
-
phosphatidylcholine
-
Solanum tuberosum
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
110000
-
SDS-PAGE, gel filtration
Solanum tuberosum
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
digalactosyldiacylglycerol + H2O
Solanum tuberosum
-
?
-
-
-
monogalactosyldiacylglycerol + H2O
Solanum tuberosum
-
?
-
-
-
Organic Solvent Stability (protein specific)
Organic Solvent
Commentary
Organism
Acetone
85% inhibition at 10%
Solanum tuberosum
Ethanol
98% inhibition at 10%
Solanum tuberosum
ether
96% inhibition at 10%
Solanum tuberosum
Purification (Commentary) (protein specific)
Commentary
Organism
520fold
Solanum tuberosum
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
leaf
-
Solanum tuberosum
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2,3-di-O-acyl-1-O-(beta-D-galactosyl)-D-glycerol + H2O
-
94293
Solanum tuberosum
?
-
-
-
?
digalactosyldiacylglycerol + H2O
44% of the activity with monogalactosyldiacylglycerol
94293
Solanum tuberosum
digalactosylglycerol + fatty acid
-
-
-
?
digalactosyldiacylglycerol + H2O
-
94293
Solanum tuberosum
?
-
-
-
-
digalactosylmonoacylglycerol + H2O
146% of the activity with monogalactosyldiacylglycerol
94293
Solanum tuberosum
digalactosylglycerol + fatty acid
-
-
-
?
lysophosphatidylcholine + H2O
26% of the activity with monogalactosyldiacylglycerol
94293
Solanum tuberosum
?
-
-
-
?
lysophosphatidylethanolamine + H2O
6% of the activity with monogalactosyldiacylglycerol
94293
Solanum tuberosum
?
-
-
-
?
lysophosphatidylglycerol + H2O
89% of the activity with monogalactosyldiacylglycerol
94293
Solanum tuberosum
?
-
-
-
?
monogalactosyldiacylglycerol + H2O
-
94293
Solanum tuberosum
galactosylglycerol + fatty acid
-
-
-
?
monogalactosyldiacylglycerol + H2O
-
94293
Solanum tuberosum
?
-
-
-
-
monogalactosylmonoacylglycerol + H2O
394% of the activity with monogalactosyldiacylglycerol
94293
Solanum tuberosum
monogalactosylglycerol + fatty acid
-
-
-
?
phosphatidic acid + H2O
8% of the activity with monogalactosyldiacylglycerol
94293
Solanum tuberosum
?
-
-
-
?
phosphatidylcholine + H2O
14% of the activity with monogalactosyldiacylglycerol
94293
Solanum tuberosum
?
-
-
-
?
phosphatidylethanolamine + H2O
1% of the activity with monogalactosyldiacylglycerol
94293
Solanum tuberosum
?
-
-
-
?
phosphatidylglycerol + H2O
77% of the activity with monogalactosyldiacylglycerol
94293
Solanum tuberosum
?
-
-
-
?
sulfoquinovosyldiaclglycerol + H2O
43% of the activity with monogalactosyldiacylglycerol
94293
Solanum tuberosum
?
-
-
-
?
triacylglycerol + H2O
below 0.1% of the activity with monogalactosyldiacylglycerol
94293
Solanum tuberosum
?
-
-
-
?
Temperature Optimum [C] (protein specific)
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
30
-
phosphatidylcholine as substrate
Solanum tuberosum
35
-
monogalactosyldiacylglycerol as substrate
Solanum tuberosum
Temperature Stability [C] (protein specific)
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
60
-
completely inactivated after 10 min at pH 8.0, 70% of its activity retained at pH 5.5
Solanum tuberosum
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
5.5
-
monogalactosyldiacylglycerol as substrate, at 35C, shift to pH 6.0 in the presence of Triton X-100
Solanum tuberosum
6
-
monogalactosyldiacylglycerol as substrate, at 35C, shift from pH 5.5 in the presence of Triton X-100
Solanum tuberosum
Other publictions for EC 3.1.1.26
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
749696
Hashiro
A novel galactolipase from a ...
Parachlorella kessleri 11h, Parachlorella kessleri
Appl. Microbiol. Biotechnol.
102
1711-1723
2018
1
-
1
-
-
-
8
3
1
2
1
9
-
3
-
1
1
-
-
-
1
-
18
2
1
-
1
2
1
-
1
-
-
1
-
1
-
1
-
-
-
-
-
8
-
3
1
2
1
9
-
-
1
1
-
-
1
-
18
2
1
-
1
2
1
-
1
1
-
2
2
-
2
2
749967
Belhaj
Galactolipase activity of Tal ...
Thermomyces dupontii, Thermomyces dupontii Stolk
Biochim. Biophys. Acta
1863
1006-1015
2018
-
-
-
-
-
-
-
-
-
-
-
-
-
6
-
-
1
-
-
-
3
-
4
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
3
-
4
-
1
-
-
-
1
-
-
-
-
1
1
-
-
-
749950
Jallouli
-
The galactolipase activity of ...
Nectria haematococca mpVI, Nectria haematococca mpVI 77-13-4, Nectria haematococca mpVI ATCC MYA-4622, Nectria haematococca mpVI FGSC 9596
Biochim. Biophys. Acta
1851
282-289
2015
-
-
1
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
1
2
-
16
-
1
-
-
-
1
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
2
-
16
-
1
-
-
-
1
1
-
-
-
2
2
-
-
-
729670
Amara
-
The galactolipase activity of ...
Cavia porcellus, Thermomyces lanuginosus
Eur. J. Lipid Sci. Technol.
115
442-451
2013
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
1
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
729279
Amara
Inhibition of phospholipase A1 ...
Homo sapiens
Biochim. Biophys. Acta
1821
1379-1385
2012
-
-
1
-
-
-
1
-
-
-
3
-
-
2
-
1
1
-
-
1
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
-
-
3
-
-
-
1
1
-
1
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
715824
Christeller
Galactolipase, phospholipase a ...
Ephestia kuehniella, Epiphyas postvittana, Galleria mellonella, Helicoverpa armigera, Plodia interpunctella, Tineola bisselliella
J. Insect Physiol.
57
1232-1239
2011
-
-
-
-
-
-
-
-
-
-
-
-
-
14
-
-
-
-
-
18
-
-
6
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
18
-
-
6
-
-
-
-
-
3
-
-
-
2
-
-
2
-
-
714318
Amara
Lipolysis of natural long chai ...
Cavia porcellus, Homo sapiens
Biochim. Biophys. Acta
1801
508-516
2010
-
2
2
-
-
-
-
-
-
-
-
-
-
6
-
-
2
-
-
2
8
-
6
-
-
-
-
-
-
-
-
-
-
-
-
-
2
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
2
8
-
6
-
-
-
-
-
-
-
-
-
-
2
2
-
-
-
691003
Eydoux
Structure of human pancreatic ...
Homo sapiens
Biochemistry
47
9553-9564
2008
1
-
1
1
1
-
1
-
-
-
2
1
-
1
-
1
1
-
-
1
10
-
11
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
1
1
-
-
1
-
-
-
-
2
1
-
-
1
1
-
1
10
-
11
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
691901
De Caro
Occurrence of pancreatic lipas ...
Canis lupus familiaris, Cavia porcellus, Equus caballus, Felis silvestris, Homo sapiens, Myocastor coypus, no activity in Bos taurus, no activity in Capra hircus, no activity in Meleagris gallopavo, no activity in Ovis aries, no activity in Struthio camelus, Oryctolagus cuniculus, Rattus norvegicus, Sus scrofa
Comp. Biochem. Physiol. B
150
1-9
2008
-
-
-
-
-
-
-
-
-
-
9
-
-
25
-
-
9
-
-
9
-
-
66
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
11
-
-
-
-
11
-
11
-
-
66
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
691980
Hyun
Cooperation and functional div ...
Arabidopsis thaliana
Dev. Cell
14
183-192
2008
1
-
-
-
-
-
-
-
1
-
-
-
-
2
-
-
-
-
-
1
-
-
3
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
3
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
692412
Illijas
-
Purification and characterizat ...
Gracilaria vermiculophylla
Fish. Sci.
74
670-676
2008
-
-
-
-
-
-
4
-
-
4
2
1
-
1
-
-
1
-
-
-
2
-
7
1
1
1
-
-
1
1
1
-
-
-
-
-
-
-
-
-
-
-
-
4
-
-
-
4
2
1
-
-
-
1
-
-
2
-
7
1
1
1
-
-
1
1
1
-
-
-
-
-
-
-
694587
Matos
Effects of progressive drought ...
Arabidopsis thaliana
Physiol. Plant.
134
110-120
2008
1
-
1
-
-
-
-
-
-
-
-
-
-
5
-
-
-
-
-
6
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
6
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
694612
Kaniuga
Chilling response of plants: i ...
no activity in Solanum x ajanhuiri, Solanum lycopersicum, Solanum tuberosum, Zea mays
Plant Biol.
10
171-184
2008
3
-
-
-
-
-
-
-
3
-
-
3
-
5
-
-
-
-
-
3
-
-
3
-
-
3
3
-
2
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
3
-
-
3
-
-
-
-
-
3
-
-
3
-
-
3
3
-
2
-
-
-
-
-
-
-
-
-
672933
Lafont
Syntheses of an a-D-Gal-(1->6) ...
Cavia porcellus
Carbohydr. Res.
341
695-704
2006
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
673082
Lion
Phospholipases and Galactolipa ...
Gracilaria chilensis
Chembiochem
7
457-462
2006
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
664006
Sias
Human pancreatic lipase-relate ...
Homo sapiens
Biochemistry
43
10138-10148
2004
-
-
1
-
-
-
-
-
-
-
-
-
-
6
-
-
1
-
-
2
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
2
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
664182
De Caro
Characterization of pancreatic ...
Homo sapiens
Biochim. Biophys. Acta
1701
89-99
2004
-
-
-
-
-
-
-
-
-
-
1
1
-
5
-
-
1
-
-
1
-
-
4
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
1
-
1
-
-
4
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
650643
Terasaki
Glycerolipid acyl hydrolase ac ...
Cladosiphon okamuranus
Biosci. Biotechnol. Biochem.
67
1986-1989
2003
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
651276
Matos
A novel patatin-like gene stim ...
Vigna unguiculata
FEBS Lett.
491
188-192
2001
-
-
1
-
-
-
-
-
-
-
2
-
-
4
-
-
-
-
-
1
-
-
4
-
-
-
-
-
1
-
-
-
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
1
-
-
4
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
651556
Kim
Thermal inactivation kinetics ...
Daucus carota, Spinacia oleracea
J. Agric. Food Chem.
49
2241-2248
2001
-
-
-
-
-
-
-
-
-
-
-
-
-
5
-
-
-
-
-
-
-
-
2
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
649811
Matos
A patatin-like protein with ga ...
Vigna unguiculata
Biochem. Soc. Trans.
28
779-781
2000
-
-
1
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
1
-
-
3
-
1
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
3
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
94300
Kaniuga
-
Degradation of leaf polar lipi ...
Zea mays
Acta Physiol. Plant.
21
45-56
1999
-
-
-
-
-
-
-
-
1
-
-
2
-
1
-
-
-
-
-
-
-
-
7
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
-
-
7
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
94301
Kaniuga
Galactolipase activity but not ...
Zea mays
Plant Cell Rep.
17
897-901
1998
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
4
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
94299
Sahsah
Purification and characterizat ...
Vigna unguiculata
Biochim. Biophys. Acta
1215
66-73
1994
-
-
-
-
-
-
-
1
-
2
2
2
-
1
-
-
1
-
-
-
4
-
7
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
2
2
2
-
-
-
1
-
-
4
-
7
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
94287
O'Sullivan
-
A galactolipase activity assoc ...
Triticum aestivum
J. Plant Physiol.
131
393-404
1987
-
-
-
-
-
2
5
-
2
-
-
2
-
1
-
-
-
-
-
1
-
1
3
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
5
-
-
2
-
-
2
-
-
-
-
-
1
-
1
3
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
94288
Gemel
Comparison of galactolipase ac ...
Beta vulgaris, Brassica napus, Brassica oleracea, Capsicum annuum, Cucumis sativus, Dahlia pinnata, Lupinus angustifolius, Medicago sativa subsp. x varia, Nicotiana tabacum, no activity in Brassica rapa, no activity in Cucumis melo, no activity in Cucurbita maxima, no activity in Cucurbita pepo, no activity in Glycine max, no activity in Hordeum vulgare, no activity in Passiflora edulis, no activity in Passiflora edulis Sims, no activity in Passiflora quadrangularis, no activity in Passiflora quadrangularis L., Phaseolus coccineus, Phaseolus vulgaris, Pisum arvense, Pisum arvense L., Pisum sativum, Solanum habrochaites, Solanum lycopersicum, Solanum peruvianum, Spinacia oleracea, Vicia faba, Zea mays
Eur. J. Biochem.
166
229-233
1987
-
-
-
-
-
-
-
-
19
-
-
40
-
37
-
-
-
-
-
19
19
-
80
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
19
-
-
40
-
-
-
-
-
19
19
-
80
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
94289
Kaniuga
-
Galactolipase activity and fre ...
Brassica oleracea, no activity in Lactuca sativa, Phaseolus vulgaris, Pisum arvense, Pisum sativum, Solanum lycopersicum, Spinacia oleracea, Vicia faba, Zea mays
FEBS Lett.
171
55-58
1984
-
-
-
-
-
-
-
-
8
-
-
16
-
9
-
-
-
-
-
8
8
-
32
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
8
-
-
16
-
-
-
-
-
8
8
-
32
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
94290
Matsuda
-
Purification of a galactolipas ...
Oryza sativa
Agric. Biol. Chem.
43
463-469
1979
1
-
-
-
-
-
1
-
1
-
-
2
-
1
-
-
1
-
-
2
1
-
5
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
-
-
1
-
-
2
-
-
-
1
-
2
1
-
5
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
94291
Matsuda
-
Purification of a lipolytic ac ...
Phaseolus vulgaris
Agric. Biol. Chem.
43
563-570
1979
1
-
-
-
-
-
4
2
-
-
1
2
3
1
-
-
1
-
-
1
1
1
15
-
1
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
4
-
2
-
-
1
2
3
-
-
1
-
1
1
1
15
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
94292
Matsuda
-
Enzymatic properties of a gala ...
Oryza sativa
Agric. Biol. Chem.
43
697-703
1979
-
-
-
-
-
-
9
1
-
1
1
2
3
1
-
-
-
-
-
1
-
-
9
-
1
1
1
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
9
-
1
-
1
1
2
3
-
-
-
-
1
-
-
9
-
1
1
1
-
1
1
-
-
-
-
-
-
-
-
94293
Matsuda
Purification and properties of ...
Solanum tuberosum
Biochim. Biophys. Acta
573
155-165
1979
1
-
-
-
-
-
4
2
-
-
1
2
3
2
-
-
1
-
-
1
-
-
16
-
2
-
1
-
2
-
-
-
-
-
-
1
-
-
-
-
-
-
-
4
-
2
-
-
1
2
3
-
-
1
-
1
-
-
16
-
2
-
1
-
2
-
-
-
-
-
-
-
-
-
94294
Hirayama
Purification and properties of ...
Solanum tuberosum
Biochim. Biophys. Acta
384
127-137
1975
-
-
-
-
-
-
5
2
-
-
1
2
-
2
-
-
-
-
-
3
-
-
16
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
5
-
2
-
-
1
2
-
-
-
-
-
3
-
-
16
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
94296
Bajwa
Degradation of monogalactosyl ...
Cavia porcellus, Ovis aries, Rattus norvegicus
Biochem. J.
144
177-187
1974
-
-
-
-
-
-
-
-
-
-
-
6
-
3
-
-
-
-
-
6
-
-
12
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
6
-
-
-
-
-
6
-
-
12
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
94295
Sastry
-
Monogalactosyl and digalactosy ...
Phaseolus coccineus
Methods Enzymol.
14
204-208
1969
-
-
-
-
-
-
-
2
-
1
-
2
1
1
-
-
-
-
-
-
-
-
6
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
1
-
2
1
-
-
-
-
-
-
-
6
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
94297
Helmsing
Purification and properties of ...
Phaseolus coccineus
Biochim. Biophys. Acta
178
519-533
1969
-
-
-
-
-
-
4
2
-
1
1
2
-
1
-
-
1
-
-
-
-
1
4
-
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
4
1
2
-
1
1
2
-
-
-
1
-
-
-
1
4
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
94298
Helmsing
Hydrolysis of galactolipids by ...
Spinacia oleracea
Biochim. Biophys. Acta
144
470-472
1967
-
-
-
-
-
-
-
-
-
-
-
2
-
1
-
-
-
-
-
1
-
-
4
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
1
-
-
4
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-