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Literature summary for 3.1.1.26 extracted from

  • Jallouli, R.; Othman, H.; Amara, S.; Parsiegla, G.; Carriere, F.; Srairi-Abid, N.; Gargouri, Y.; Bezzine, S.
    The galactolipase activity of Fusarium solani (phospho)lipase (2015), Biochim. Biophys. Acta, 1851, 282-289 .
No PubMed abstract available
Search for more literature for 3.1.1.26

Cloned(Commentary)

Cloned (Comment) Organism
recombinant expression of enzyme FSl in Escherichia coli strain XL1-Blue Fusarium vanettenii

Organism

Organism UniProt Comment Textmining
Fusarium vanettenii C7YUZ1 i.e. Nectria haematococca
-
Fusarium vanettenii 77-13-4 C7YUZ1 i.e. Nectria haematococca
-
Fusarium vanettenii ATCC MYA-4622 C7YUZ1 i.e. Nectria haematococca
-
Fusarium vanettenii FGSC 9596 C7YUZ1 i.e. Nectria haematococca
-
Fusarium vanettenii MPVI C7YUZ1 i.e. Nectria haematococca
-

Source Tissue

Source Tissue Comment Organism Textmining
mycelium
-
 Fusarium vanettenii
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3785
-
 substrate 1,2-octanoyl-3-O-alpha-D-galactosyl-(1->6)-beta-D-galactosyl-sn-glycerol, pH 8.0, 37°C Fusarium vanettenii
4658
-
 substrate 1,2-octanoyl-3-O-alpha-D-galactosyl-sn-glycerol, pH 8.0, 37°C Fusarium vanettenii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1,2-octanoyl-3-O-alpha-D-galactosyl-(1->6)-beta-D-galactosyl-sn-glycerol + H2O
-
 Fusarium vanettenii 2-octanoyl-3-O-alpha-D-galactosyl-(1->6)-beta-D-galactosyl-sn-glycerol + octanoate
-
 ?
1,2-octanoyl-3-O-alpha-D-galactosyl-(1->6)-beta-D-galactosyl-sn-glycerol + H2O
-
 Fusarium vanettenii MPVI 2-octanoyl-3-O-alpha-D-galactosyl-(1->6)-beta-D-galactosyl-sn-glycerol + octanoate
-
 ?
1,2-octanoyl-3-O-alpha-D-galactosyl-(1->6)-beta-D-galactosyl-sn-glycerol + H2O
-
 Fusarium vanettenii 77-13-4 2-octanoyl-3-O-alpha-D-galactosyl-(1->6)-beta-D-galactosyl-sn-glycerol + octanoate
-
 ?
1,2-octanoyl-3-O-alpha-D-galactosyl-(1->6)-beta-D-galactosyl-sn-glycerol + H2O
-
 Fusarium vanettenii ATCC MYA-4622 2-octanoyl-3-O-alpha-D-galactosyl-(1->6)-beta-D-galactosyl-sn-glycerol + octanoate
-
 ?
1,2-octanoyl-3-O-alpha-D-galactosyl-(1->6)-beta-D-galactosyl-sn-glycerol + H2O
-
 Fusarium vanettenii FGSC 9596 2-octanoyl-3-O-alpha-D-galactosyl-(1->6)-beta-D-galactosyl-sn-glycerol + octanoate
-
 ?
1,2-octanoyl-3-O-alpha-D-galactosyl-sn-glycerol + H2O
-
 Fusarium vanettenii 2-octanoyl-3-O-alpha-D-galactosyl-sn-glycerol + octanoate
-
 ?
1,2-octanoyl-3-O-alpha-D-galactosyl-sn-glycerol + H2O
-
 Fusarium vanettenii MPVI 2-octanoyl-3-O-alpha-D-galactosyl-sn-glycerol + octanoate
-
 ?
1,2-octanoyl-3-O-alpha-D-galactosyl-sn-glycerol + H2O
-
 Fusarium vanettenii 77-13-4 2-octanoyl-3-O-alpha-D-galactosyl-sn-glycerol + octanoate
-
 ?
1,2-octanoyl-3-O-alpha-D-galactosyl-sn-glycerol + H2O
-
 Fusarium vanettenii ATCC MYA-4622 2-octanoyl-3-O-alpha-D-galactosyl-sn-glycerol + octanoate
-
 ?
1,2-octanoyl-3-O-alpha-D-galactosyl-sn-glycerol + H2O
-
 Fusarium vanettenii FGSC 9596 2-octanoyl-3-O-alpha-D-galactosyl-sn-glycerol + octanoate
-
 ?
additional information the purified (phospho)lipase of Fusariumsolani (FSL) is active on both triglycerides and phospholipids. But the enzyme is also capable of hydrolyzing galactolipids. FSL hydrolyzes synthetic medium chain-length substrates monogalactosyldiacylglycerol and digalactosyldiacylglycerol at high rates, as well as natural long chain monogalactosyldiacylglycerol extracted from leek leaves. It is the microbial enzyme with the highest activity on galactolipids identified so far with a level of activity comparable to that of pancreatic lipase-related protein 2. Enzyme FSL has a broad substrate specificity towards different classes of lipids, triglycerides, phospholipids and galactolipids Fusarium vanettenii ?
-
 ?
additional information the purified (phospho)lipase of Fusariumsolani (FSL) is active on both triglycerides and phospholipids. But the enzyme is also capable of hydrolyzing galactolipids. FSL hydrolyzes synthetic medium chain-length substrates monogalactosyldiacylglycerol and digalactosyldiacylglycerol at high rates, as well as natural long chain monogalactosyldiacylglycerol extracted from leek leaves. It is the microbial enzyme with the highest activity on galactolipids identified so far with a level of activity comparable to that of pancreatic lipase-related protein 2. Enzyme FSL has a broad substrate specificity towards different classes of lipids, triglycerides, phospholipids and galactolipids Fusarium vanettenii MPVI ?
-
 ?
additional information the purified (phospho)lipase of Fusariumsolani (FSL) is active on both triglycerides and phospholipids. But the enzyme is also capable of hydrolyzing galactolipids. FSL hydrolyzes synthetic medium chain-length substrates monogalactosyldiacylglycerol and digalactosyldiacylglycerol at high rates, as well as natural long chain monogalactosyldiacylglycerol extracted from leek leaves. It is the microbial enzyme with the highest activity on galactolipids identified so far with a level of activity comparable to that of pancreatic lipase-related protein 2. Enzyme FSL has a broad substrate specificity towards different classes of lipids, triglycerides, phospholipids and galactolipids Fusarium vanettenii 77-13-4 ?
-
 ?
additional information the purified (phospho)lipase of Fusariumsolani (FSL) is active on both triglycerides and phospholipids. But the enzyme is also capable of hydrolyzing galactolipids. FSL hydrolyzes synthetic medium chain-length substrates monogalactosyldiacylglycerol and digalactosyldiacylglycerol at high rates, as well as natural long chain monogalactosyldiacylglycerol extracted from leek leaves. It is the microbial enzyme with the highest activity on galactolipids identified so far with a level of activity comparable to that of pancreatic lipase-related protein 2. Enzyme FSL has a broad substrate specificity towards different classes of lipids, triglycerides, phospholipids and galactolipids Fusarium vanettenii ATCC MYA-4622 ?
-
 ?
additional information the purified (phospho)lipase of Fusariumsolani (FSL) is active on both triglycerides and phospholipids. But the enzyme is also capable of hydrolyzing galactolipids. FSL hydrolyzes synthetic medium chain-length substrates monogalactosyldiacylglycerol and digalactosyldiacylglycerol at high rates, as well as natural long chain monogalactosyldiacylglycerol extracted from leek leaves. It is the microbial enzyme with the highest activity on galactolipids identified so far with a level of activity comparable to that of pancreatic lipase-related protein 2. Enzyme FSL has a broad substrate specificity towards different classes of lipids, triglycerides, phospholipids and galactolipids Fusarium vanettenii FGSC 9596 ?
-
 ?
phosphatidylcholine + H2O from henn egg yolk Fusarium vanettenii ?
-
 ?
phosphatidylcholine + H2O from henn egg yolk Fusarium vanettenii MPVI ?
-
 ?
phosphatidylcholine + H2O from henn egg yolk Fusarium vanettenii 77-13-4 ?
-
 ?
phosphatidylcholine + H2O from henn egg yolk Fusarium vanettenii ATCC MYA-4622 ?
-
 ?
phosphatidylcholine + H2O from henn egg yolk Fusarium vanettenii FGSC 9596 ?
-
 ?

Synonyms

Synonyms Comment Organism
FSL
-
 Fusarium vanettenii
NECHADRAFT_49364
-
 Fusarium vanettenii

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Fusarium vanettenii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Fusarium vanettenii

pH Range

pH Minimum pH Maximum Comment Organism
7.5 10 over 80% of maximal activity at Fusarium vanettenii

General Information

General Information Comment Organism
evolution the enzyme structure belongs to lipase 3 family Fusarium vanettenii
additional information structure-activity relationships of FSL, three-simensional modeling of the enzyme, in silico docking of medium chains substrates MGDG and DGDG and phospholipid in the active site of enzyme FSL, homology modeling of FSL, detailed overview Fusarium vanettenii