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Literature summary for 3.1.1.26 extracted from

  • Illijas, M.I.; Terasaki, M.; Nakamura, R.; Iijima, N.; Hara, A.; Fusetani, N.; Itabashi, Y.
    Purification and characterization of glycerolipid acyl-hydrolase from the red alga Gracilaria vermiculophylla (2008), Fish. Sci., 74, 670-676.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
Ca2+ 38.9% residual activity in the presence of 50 mM Ca2+ Agarophyton vermiculophyllum
Mg2+ 90.7% residual activity in the presence of 50 mM Mg2+ Agarophyton vermiculophyllum
Mn2+ 12.2% residual activity in the presence of 50 mM Mn2+ Agarophyton vermiculophyllum
Zn2+ 16.3% residual activity in the presence of 50 mM Zn 2+ Agarophyton vermiculophyllum

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ 130% relative activity in the presence of 5 mM Ca2+ Agarophyton vermiculophyllum
Mg2+ 151% relative activity in the presence of 5 mM Mg2+ Agarophyton vermiculophyllum
Mn2+ 128% relative activity in the presence of 10 mM Mn2+ Agarophyton vermiculophyllum
Zn2+ 128% relative activity in the presence of 1 mM Zn2+ Agarophyton vermiculophyllum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
20000
-
2 * 20000, SDS-PAGE Agarophyton vermiculophyllum
40000
-
gel filtration Agarophyton vermiculophyllum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Agarophyton vermiculophyllum the enzyme hydrolyzes the membrane lipids to release various saturated and unsaturated fatty acids, including arachidonic acid as substrate for prostaglandin synthesis ?
-
?

Organism

Organism UniProt Comment Textmining
Agarophyton vermiculophyllum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation, DEAE-Toyopearl 650 M colum chromatography, and HW-55F gel filtration Agarophyton vermiculophyllum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.000194
-
crude extract Agarophyton vermiculophyllum
0.00367
-
after 18.9fold purification Agarophyton vermiculophyllum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1,2-di-O-dodecanoyl-3-O-beta-D-galactopyranosyl-sn-glycerol + H2O
-
Agarophyton vermiculophyllum 2,3-dihydroxypropyl beta-D-galactopyranoside + dodecanoate
-
?
3-O-(6-O-alpha-D-galactopyranosyl-beta-D-galactopyranosyl)-1,2-di-O-dodecanoyl-sn-glycerol + H2O
-
Agarophyton vermiculophyllum 2,3-dihydroxypropyl 6-O-beta-D-galactopyranosyl-beta-D-galactopyranoside + 2 dodecanoate
-
?
digalactosyldiacylglycerol + H2O
-
Agarophyton vermiculophyllum digalactosylglycerol + fatty acid
-
?
monogalactosyldiacylglycerol + H2O high activity Agarophyton vermiculophyllum galactosylglycerol + fatty acid
-
?
additional information the enzyme hydrolyzes the membrane lipids to release various saturated and unsaturated fatty acids, including arachidonic acid as substrate for prostaglandin synthesis Agarophyton vermiculophyllum ?
-
?
phosphatidylcholine + H2O highest activity Agarophyton vermiculophyllum ?
-
?
phosphatidylglycerol + H2O
-
Agarophyton vermiculophyllum ?
-
?

Subunits

Subunits Comment Organism
homodimer 2 * 20000, SDS-PAGE Agarophyton vermiculophyllum

Synonyms

Synonyms Comment Organism
glycerolipid acyl-hydrolase
-
Agarophyton vermiculophyllum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
-
Agarophyton vermiculophyllum

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
10 50 the enzymatic activity increases gradually from 10 to 30°C, drastically increases at 37°C and then continuously decreases over 37°C Agarophyton vermiculophyllum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7 8
-
Agarophyton vermiculophyllum

pH Range

pH Minimum pH Maximum Comment Organism
4 10
-
Agarophyton vermiculophyllum

pH Stability

pH Stability pH Stability Maximum Comment Organism
10
-
the enzyme is inactive above pH 10.0 Agarophyton vermiculophyllum